Allele Dmel\mwh^GD8165

General Information
Symbol	Dmel\mwh^GD8165	Species	D. melanogaster
Name		FlyBase ID	FBal0200506
Feature type	allele	Associated gene	Dmel\mwh

Map ( GBrowse )
Allele class
Mutagen	in vitro construct - RNAi, in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Dickson et al., 2007.7.18) in vitro construct - RNAi (Dickson et al., 2007.7.18)
Mutations Mapped to the Genome

Type Location Additional Notes References RNAi reagent :1..368
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an inverted repeat. (Dickson et al., 2007.7.18)
Carried in construct	P{GD8165} (Dickson et al., 2007.7.18, Yan et al., 2008, Strutt and Warrington, 2008, Mummery-Widmer et al., 2009, Gault et al., 2012)
Associated Sequence Features	dsRNA-GD8165
Cytology
Phenotypic Data
Phenotypic Class
	body color defective, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) viable, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) visible, with Scer\GAL4^ap-md544 (Yan et al., 2008) visible, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) visible, with Scer\GAL4^ptc-559.1 (Strutt and Warrington, 2008)
Phenotype Manifest In
	mesothoracic tergum, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) trichogen cell, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) wing hair \| supernumerary, with Scer\GAL4^ap-md544 (Yan et al., 2008) wing hair \| supernumerary, with Scer\GAL4^en-e16E (Gault et al., 2012) wing hair \| supernumerary, with Scer\GAL4^ptc-559.1 (Strutt and Warrington, 2008)
Detailed Description
	Statement Reference Overexpression of mwh[GD8165] via Scer\GAL4[en-e16E] results in wing hair abnormalities. (Gault et al., 2012) Expression under the control of Scer\GAL4[pnr-MD237] results in a colour difference between the central Scer\GAL4[pnr-MD237] expression domain of the notum and the surrounding lateral region in 0% or 30-40% of the Scer\GAL4[pnr-MD237] expression domain, depending on the insertion line used. Expression under the control of Scer\GAL4[pnr-MD237] results in bristle morphology defects on the notum in 0% or 20-30% of the Scer\GAL4[pnr-MD237] expression domain, depending on the insertion line used. (Mummery-Widmer et al., 2009) Flies expressing mwh[GD8165] under the control of Scer\GAL4[ptc-559.1] have a multiple wing hair phenotype. (Strutt and Warrington, 2008) Expression of mwh[GD8165] under the control of Scer\GAL4[ap-md544] results in a multiple wing hair phenotype. (Yan et al., 2008)
External Data
Linkouts	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 8165
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement Reference Scer\GAL4^en-e16E, mwh^GD8165 has wing hair \| supernumerary phenotype, suppressible by Scer\GAL4^en-e16E/gish^{Scer\UAS.RB.T:Hsap\MYC} (Gault et al., 2012)
NOT suppressed by
Statement Reference Scer\GAL4^en-e16E, mwh^GD8165 has wing hair \| supernumerary phenotype, non-suppressible by Scer\GAL4^en-e16E/gish^{D187N.Scer\UAS.T:Hsap\MYC} (Gault et al., 2012) Scer\GAL4^en-e16E, mwh^GD8165 has wing hair \| supernumerary phenotype, non-suppressible by Scer\GAL4^en-e16E/gish^{Scer\UAS.RF.T:Hsap\MYC} (Gault et al., 2012)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of gish[Scer\UAS.RB.T:Hsap\MYC] with mwh[GD8165] via Scer\GAL4[en-e16E] suppresses the mwh[GD8165]-induced wing hair phenotype. Co-expression of gish[D187N.Scer\UAS.T:Hsap\MYC] with mwh[GD8165] via Scer\GAL4[en-e16E] fails to suppress the mwh[GD8165]-induced wing hair phenotype. Co-expression of gish[Scer\UAS.RF.T:Hsap\MYC] with mwh[GD8165] via Scer\GAL4[en-e16E] fails to suppress the mwh[GD8165]-induced wing hair phenotype. (Gault et al., 2012)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 3 )
VDRC	v41513 w¹¹¹⁸; P{GD8165}v41513 v41514 w¹¹¹⁸; P{GD8165}v41514 v45265 w¹¹¹⁸; P{GD8165}v45265
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts
	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 8165
Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	CG13913^GD8165 CG43772^GD8165 mwh^GD8165
Name Synonym
Secondary FlyBase IDs

References ( 5 )
Research paper	Gault et al., 2012, J. Cell Biol. 196(5): 605--621 Drosophila CK1-γ, gilgamesh, controls PCP-mediated morphogenesis through regulation of vesicle trafficking. [FBrf0217630] Strutt and Warrington, 2008, Development 135(18): 3103--3111 Planar polarity genes in the Drosophila wing regulate the localisation of the FH3-domain protein Multiple Wing Hairs to control the site of hair production. [FBrf0205732] Yan et al., 2008, Genetics 180(1): 219--228 The multiple-wing-hairs gene encodes a novel GBD-FH3 domain-containing protein that functions both prior to and after wing hair initiation. [FBrf0206936]
Supplementary material	Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]

Allele Dmel\mwhGD8165

Allele Dmel\mwh^GD8165