FlyBase Allele Report: Dmel\mwh[GD8165]

General Information

Symbol

Dmel\mwh^GD8165

Species

D. melanogaster

Name

FlyBase ID

FBal0200506

Feature type

allele

Associated gene

Dmel\mwh

Associated Insertion(s)

Carried in Construct

P{GD8165}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD8165}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD8165

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body color, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

abnormal locomotor behavior | adult stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

visible, with Scer\GAL4^ap-md544

(Yan et al., 2008)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

visible, with Scer\GAL4^ptc-559.1

(Strutt and Warrington, 2008)

Phenotype Manifest In

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing hair | increased number, with Scer\GAL4^ap-md544

(Yan et al., 2008)

wing hair | increased number, with Scer\GAL4^en-e16E

(Gault et al., 2012)

wing hair | increased number, with Scer\GAL4^ptc-559.1

(Strutt and Warrington, 2008)

Detailed Description

Statement

Reference

Expression of mwh^GD8165 RNAi under the control of either Scer\GAL4^pnr-MD237 or Scer\GAL4^tub.PU does not significantly alter levels of pigmentation on the abdominal tergite 5 or 6 in adult females compared to controls.

(Dembeck et al., 2015)

Overexpression of mwh^GD8165 via Scer\GAL4^en-e16E results in wing hair abnormalities.

(Gault et al., 2012)

Adults expressing mwh^GD8165 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of mwh^GD8165 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or complete lethality, depending on the particular P{GD8165} insertion line used.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in defects in locomotion.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in a colour difference between the central Scer\GAL4^pnr-MD237 expression domain of the notum and the surrounding lateral region in 0% or 30-40% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 20-30% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

Flies expressing mwh^GD8165 under the control of Scer\GAL4^ptc-559.1 have a multiple wing hair phenotype.

(Strutt and Warrington, 2008)

Expression of mwh^GD8165 under the control of Scer\GAL4^ap-md544 results in a multiple wing hair phenotype.

(Yan et al., 2008)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development