FlyBase Allele Report: Dmel\nkt[GD8239]

General Information

Symbol

Dmel\nkt^GD8239

Species

D. melanogaster

Name

FlyBase ID

FBal0200636

Feature type

allele

Associated gene

Dmel\nkt

Associated Insertion(s)

Carried in Construct

P{GD8239}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD8239}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD8239

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Scer\GAL4^Eaat1.PR, Scer\GAL80^elav.PU

(Sengupta et al., 2019)

abnormal locomotor behavior | adult stage, with Scer\GAL4^elav.PU

(Sengupta et al., 2019)

abnormal locomotor behavior | adult stage, with Scer\GAL4^NP3233

(Sengupta et al., 2019)

abnormal locomotor behavior | adult stage, with Scer\GAL4^nSyb.PU

(Sengupta et al., 2019)

abnormal locomotor behavior | adult stage, with Scer\GAL4^repo.PU

(Sengupta et al., 2019)

abnormal locomotor behavior | adult stage, with Scer\GAL4^Tub.PU

(Sengupta et al., 2019)

abnormal sleep, with Scer\GAL4^238Y

(Sengupta et al., 2019)

abnormal sleep, with Scer\GAL4^c305a

(Sengupta et al., 2019)

abnormal sleep, with Scer\GAL4^elav.PU

(Sengupta et al., 2019)

abnormal sleep, with Scer\GAL4^GMR35B12

(Sengupta et al., 2019)

abnormal sleep, with Scer\GAL4^GMR86E01

(Sengupta et al., 2019)

abnormal sleep, with Scer\GAL4^nSyb.PU

(Sengupta et al., 2019)

abnormal sleep, with Scer\GAL4^repo.PU

(Sengupta et al., 2019)

decreased sleep | adult stage, with Scer\GAL4^GMR13F02

(Draper et al., 2024)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expressing nkt^GD8239 under the control of either Scer\GAL4^repo.PU, Scer\GAL4^elav.PU or Scer\GAL4^nSyb.PU results in a significant decrease in night-time sleep, with a reciprocal increase in night-time locomotor activity, but no effect on day-time sleep; there is a significant increase in sleep latency and fragmented sleep, with significantly more but shorter sleep bouts. In the Scer\GAL4^repo.PU and Scer\GAL4^elav.PU conditions there is normal circadian rhythmicity (i.e. it is maintained under constant darkness) and normal waking activity index. In the Scer\GAL4^elav.PU and Scer\GAL4^nSyb.PU conditions there is normal sleep loss and gain after 12 h of sleep deprivation. Introducing Scer\GAL80^Mef2.mb247 rescues the sleep phenotype observed upon Scer\GAL4^nSyb.PU-driven nkt^GD8239 expression.

Expressing nkt^GD8239 under the control of Scer\GAL4^GMR86E01, Scer\GAL4^238Y, Scer\GAL4^c305a or Scer\GAL4^GMR35B12 leads to a decrease in night-time sleep, but no effect on day-time sleep.

Adulthood-only expression of nkt^GD8239 under the control of either Scer\GAL4^repo.PU or Scer\GAL4^nSyb.PU (temporally regulated by Gal80[ts] and temperature shift from 18[o]C to 30[o]C) leads to a decrease in night-time sleep, but no effect on day-time sleep. The night-time sleep is not severely fragmented: in the Scer\GAL4^nSyb.PU condition there is no effect in sleep bout number or length, while in the Scer\GAL4^nSyb.PU condition there is a decrease in sleep bout length, but not number.

Expressing nkt^GD8239 under the control of Scer\GAL4^NP3233 alone, Scer\GAL4^Tub.PU alone or Scer\GAL4^Eaat1.PR and Scer\GAL80^elav.PU combined leads to a significant increase in locomotor activity during night-time; this is not observed if nkt^GD8239 is expressed under the control of Scer\GAL4^NP2222 alone or Scer\GAL4^Mz709 alone.

(Sengupta et al., 2019)

Adults expressing CG14141^GD8239 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in viable flies or semi-lethality.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development