FlyBase Allele Report: Dmel\Dis3l2[GD9240]

General Information

Symbol

Dmel\Dis3l2^GD9240

Species

D. melanogaster

Name

FlyBase ID

FBal0201683

Feature type

allele

Associated gene

Dmel\Dis3l2

Associated Insertion(s)

Carried in Construct

P{GD9240}

Also Known As

UAS-dis3L2^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD9240}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD9240

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

hyperplasia | adult stage, with Scer\GAL4^69B

(Towler et al., 2016)

hyperplasia | adult stage, with Scer\GAL4^Act5C.PI

(Towler et al., 2016)

hyperplasia | adult stage, with Scer\GAL4^da.G32

(Towler et al., 2016)

hyperplasia | adult stage, with Scer\GAL4^en.PU

(Towler et al., 2016)

hyperplasia | adult stage, with Scer\GAL4^nub-AC-62

(Towler et al., 2016)

hyperplasia | adult stage, with Scer\GAL4^αTub84B.PL

(Towler et al., 2016)

hyperplasia | adult stage | cell non-autonomous, with Scer\GAL4^en.PU

(Towler et al., 2016)

hyperplasia | third instar larval stage, with Scer\GAL4^69B

(Towler et al., 2016)

hyperplasia | third instar larval stage, with Scer\GAL4^nub-AC-62

(Towler et al., 2016)

increased occurrence of cell division | larval stage, with Scer\GAL4^αTub84B.PL

(Towler et al., 2020)

increased occurrence of cell division | third instar larval stage, with Scer\GAL4^69B

(Towler et al., 2016)

increased occurrence of cell division | third instar larval stage, with Scer\GAL4^nub-AC-62

(Towler et al., 2016)

increased size | adult stage, with Scer\GAL4^αTub84B.PL

(Towler et al., 2020)

increased size | larval stage, with Scer\GAL4^αTub84B.PL

(Towler et al., 2020)

male sterile, with Scer\GAL4^αTub84B.PL

(Towler et al., 2020)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

wing, with Scer\GAL4^69B

(Towler et al., 2016)

wing, with Scer\GAL4^Act5C.PI

(Towler et al., 2016)

wing, with Scer\GAL4^da.G32

(Towler et al., 2016)

wing, with Scer\GAL4^en.PU

(Towler et al., 2016)

wing, with Scer\GAL4^nub-AC-62

(Towler et al., 2016)

wing, with Scer\GAL4^αTub84B.PL

(Towler et al., 2020, Towler et al., 2016)

wing | cell non-autonomous, with Scer\GAL4^en.PU

(Towler et al., 2016)

wing disc, with Scer\GAL4^αTub84B.PL

(Towler et al., 2020)

wing disc | third instar larval stage, with Scer\GAL4^69B

(Towler et al., 2016)

wing disc | third instar larval stage, with Scer\GAL4^nub-AC-62

(Towler et al., 2016)

Detailed Description

Statement

Reference

Expressing Dis3l2^GD9240 under the control of Scer\GAL4^69B leads to a large wing.

(Towler et al., 2020)

Expression of Dis3l2^GD9240 under the control of any of the following: Scer\GAL4^69B, Scer\GAL4^nub-AC-62 or Scer\GAL4^da.G32 results in a significant increase of adult wing size, expression driven by Scer\GAL4^en.PU leads to enlarged posterior wing compartment but to a lesser extent also the anterior compartment. Expression under either Scer\GAL4^Act5C.PI or Scer\GAL4^αTub84B.PL also increases the adult wing size even though the overall body mass is not different from the controls (with Scer\GAL4^Act5C.PI). Expression of Dis3l2^GD9240 (with Scer\GAL4^69B or Scer\GAL4^nub-AC-62) increases the wing cell number whereas the cell density is not changed relative to controls. Expression with the Scer\GAL4^69B driver combined with Gal80[ts] to exert temporal control during second and early third larval instar leads to increased wing size in the adults, but from mid third larval instar onward the Dis3l2^GD9240 expression fails to induce wing overgrowth. Larval wing disc expressing Dis3l2^GD9240 (either with the Scer\GAL4^69B driver or with Scer\GAL4^nub-AC-62) are enlarged compared to controls due to increase in cell proliferation (assessed by pH3 staining)

(Towler et al., 2016)

Adults expressing CG16940^GD9240 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Dis3l2^GD9240, Scer\GAL4^69B has hyperplasia | adult stage phenotype, enhanceable by Df(3L)H99/+

(Towler et al., 2016)

Suppressor of

Statement

Reference

Scer\GAL4^69B/Dis3l2^GD9240 is a suppressor of abnormal size | adult stage phenotype of pcm⁵

(Towler et al., 2016)

Scer\GAL4^69B/Dis3l2^GD9240 is a suppressor of abnormal size | third instar larval stage phenotype of pcm¹⁴

(Towler et al., 2016)

NOT Suppressor of

Statement

Reference

Scer\GAL4^69B/Dis3l2^GD9240 is a non-suppressor of abnormal developmental rate | larval stage phenotype of pcm¹⁴

(Towler et al., 2016)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^69B/Dis3l2^GD9240 is a suppressor of wing phenotype of pcm⁵

(Towler et al., 2016)

Scer\GAL4^69B/Dis3l2^GD9240 is a suppressor of wing disc | third instar larval stage phenotype of pcm¹⁴

(Towler et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The increased adult wing size observed in both Df(3L)H99 heterozygotes and flies expressing Dis3l2^GD9240 driven by Scer\GAL4^69B is increased still further if the manipulations are combined.

The reduced wing size observed in pcm⁵ mutant adults is fully rescued by expression of Dis3l2^GD9240 under the control of Scer\GAL4^69B in the mutant background. Similarly, the small wing discs size characteristic for pcm¹⁴ mutant third instar larvae is also suppressed by expression of Dis3l2^GD9240 but the larvae are still developmentally delayed.

(Towler et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v51853

w¹¹¹⁸; P{GD9240}v51853

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG16940^GD9240

Dis3l2^GD9240

Name Synonyms

Secondary FlyBase IDs

References (6)

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