The expression of RpIIIC160^GD4257 under the control of Scer\GAL4^TIGS-2, Scer\GAL4^bun-GSG5961 or Scer\GAL4^{elav.Switch.PO}, but not Scer\GAL4^Switch1.106, (in combination with RU486 treatment) leads to a significant increase in lifespan, as compared to controls; the Scer\GAL4^bun-GSG5961- and Scer\GAL4^TIGS-2-driven expressions also suppress the high mitotic index and the integrity defects (in smurf assays) observed in the gut of old adults, despite of no effect in young adults, as compared to age-matched controls; the Scer\GAL4^TIGS-2-driven expression has no significant effect whole body weight or female fecundity, as compared to controls.

Adults expressing CG17209^GD4257 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in early larval lethality or early pupal lethality.

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 80-90% or 90-100% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.