Somatic clones in the larval fat body expressing FarOGD3202 under the control of Scer\GAL4Scer\FRT.Rnor\CD2.Act5C do not display a detectable change in lipid droplets or cell size during starvation, as compared to controls.
Expression of FarOGD3202 under the control of Scer\GAL4Desat1.PB, or under the control of Scer\GAL4Cyp4g1-NP6073 and Scer\GAL80elav.PU to restrict expression to oenocytes, results in a decrease in the accumulation of lipid droplets in larval oenocytes during starvation, but not in fed conditions, as compared to controls.
Expression of FarOGD3202 under the control of Scer\GAL4Desat1.PB leads to a significant increase in larval oenocyte size in both fed conditions at 48 hours after hatching, and starved conditions 66 hours after hatching, but there is no difference in oenocyte size during fed conditions 66 hours after hatching, as compared to controls. Oenocyte enlargement during starvation is also associated with enlarged nuclei.
Somatic clones in larval oenocytes expressing FarOGD3202 under the control of Scer\GAL4Scer\FRT.Rnor\CD2.Act5C display a decrease in lipid droplets, as compared to control cells.
Adults expressing CG18031GD3202 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.