FlyBase Allele Report: Dmel\Stoml2[GD16706]

General Information

Symbol

Dmel\Stoml2^GD16706

Species

D. melanogaster

Name

FlyBase ID

FBal0202391

Feature type

allele

Associated gene

Dmel\Stoml2

Associated Insertion(s)

Carried in Construct

P{GD16706}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD16706}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD16706

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Parkinson's disease 2

modeled by park²⁵

(Zanon et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight, with Scer\GAL4^Tub.PU

(Zanon et al., 2017)

abnormal locomotor behavior | adult stage, with Scer\GAL4^ple.PU

(Zanon et al., 2017)

abnormal neuroanatomy | adult stage, with Scer\GAL4^ple.PU

(Zanon et al., 2017)

decreased cell number | adult stage, with Scer\GAL4^ple.PU

(Zanon et al., 2017)

male semi-fertile, with Scer\GAL4^Tub.PU

(Zanon et al., 2017)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

visible, with Df(2R)BSC695, Scer\GAL4^fln.IFM

(Zanon et al., 2017)

visible, with Df(2R)BSC770, Scer\GAL4^fln.IFM

(Zanon et al., 2017)

visible, with Scer\GAL4^fln.IFM

(Zanon et al., 2017)

visible, with Scer\GAL4^Tub.PU

(Zanon et al., 2017)

Phenotype Manifest In

dopaminergic PPL1 neuron, with Scer\GAL4^ple.PU

(Zanon et al., 2017)

thorax, with Scer\GAL4^Tub.PU

(Zanon et al., 2017)

wing, with Df(2R)BSC695, Scer\GAL4^fln.IFM

(Zanon et al., 2017)

wing, with Df(2R)BSC770, Scer\GAL4^fln.IFM

(Zanon et al., 2017)

wing, with Scer\GAL4^fln.IFM

(Zanon et al., 2017)

wing, with Scer\GAL4^Tub.PU

(Zanon et al., 2017)

Detailed Description

Statement

Reference

The expression of Stoml2^GD16706 under the control of Scer\GAL4^Tub.PU induces wing posture defects and thorax indentations in a significant proportion of individuals, a significant decrease in flight performance and a severe decrease in the progeny of males, as compared to controls. Expression under the control of Scer\GAL4^fln.IFM also leads to wing posture defects in a significant proportion of individuals, which become more frequent in combination with heterozygosity for either Df(2R)BSC695 or Df(2R)BSC770, as compared to controls.

The expression of Stoml2^GD16706 under the control of Scer\GAL4^ple.PU leads to a significant decrease in climbing index of adults and to a significant decrease in the number of dopaminergic PPL1 neurons in the adult brain, as compared to controls; the climbing defects are not enhanced by heterozygosity for either Df(2R)BSC695 or Df(2R)BSC770.

(Zanon et al., 2017)

Adults expressing CG2970^GD16706 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

16706

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^ple.PU, Stoml2^GD16706 has abnormal neuroanatomy | adult stage phenotype, enhanceable by park²⁵

(Zanon et al., 2017)

Scer\GAL4^Tub.PU, Stoml2^GD16706 has visible phenotype, enhanceable by Scer\GAL4^Tub.PU/park^KK107919

(Zanon et al., 2017)

Scer\GAL4^ple.PU, Stoml2^GD16706 has decreased cell number | adult stage phenotype, enhanceable by park²⁵

(Zanon et al., 2017)

NOT Enhanced by

Statement

Reference

Scer\GAL4^Tub.PU, Stoml2^GD16706 has abnormal flight phenotype, non-enhanceable by Scer\GAL4^Tub.PU/park^KK107919

(Zanon et al., 2017)

Scer\GAL4^Tub.PU, Stoml2^GD16706 has male semi-fertile phenotype, non-enhanceable by Scer\GAL4^Tub.PU/park^KK107919

(Zanon et al., 2017)

Suppressed by

Statement

Reference

Scer\GAL4^ple.PU, Stoml2^GD16706 has abnormal locomotor behavior | adult stage phenotype, suppressible by Scer\GAL4^ple.PU/park^UAS.cGa

(Zanon et al., 2017)

Df(2R)BSC695, Scer\GAL4^ple.PU, Stoml2^GD16706 has abnormal locomotor behavior | adult stage phenotype, suppressible by park^UAS.cGa, Scer\GAL4^ple.PU

(Zanon et al., 2017)

Df(2R)BSC770, Scer\GAL4^ple.PU, Stoml2^GD16706 has abnormal locomotor behavior | adult stage phenotype, suppressible by park^UAS.cGa, Scer\GAL4^ple.PU

(Zanon et al., 2017)

Scer\GAL4^fln.IFM, Stoml2^GD16706 has visible phenotype, suppressible by Scer\GAL4^fln.IFM/park^UAS.cGa

(Zanon et al., 2017)

Df(2R)BSC695, Scer\GAL4^fln.IFM, Stoml2^GD16706 has visible phenotype, suppressible by park^UAS.cGa, Scer\GAL4^fln.IFM

(Zanon et al., 2017)

Df(2R)BSC770, Scer\GAL4^fln.IFM, Stoml2^GD16706 has visible phenotype, suppressible by park^UAS.cGa, Scer\GAL4^fln.IFM

(Zanon et al., 2017)

Enhancer of

Statement

Reference

Stoml2^GD16706/Scer\GAL4^Tub.PU is an enhancer of visible phenotype of Scer\GAL4^Tub.PU, park^KK107919

(Zanon et al., 2017)

Scer\GAL4^ple.PU/Stoml2^GD16706 is an enhancer of abnormal neuroanatomy | adult stage phenotype of park²⁵

(Zanon et al., 2017)

Scer\GAL4^ple.PU/Stoml2^GD16706 is an enhancer of decreased cell number | adult stage phenotype of park²⁵

(Zanon et al., 2017)

NOT Enhancer of

Statement

Reference

Stoml2^GD16706/Scer\GAL4^Tub.PU is a non-enhancer of abnormal flight phenotype of Scer\GAL4^Tub.PU, park^KK107919

(Zanon et al., 2017)

Stoml2^GD16706/Scer\GAL4^Tub.PU is a non-enhancer of male semi-fertile phenotype of Scer\GAL4^Tub.PU, park^KK107919

(Zanon et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^ple.PU, Stoml2^GD16706 has dopaminergic PPL1 neuron phenotype, enhanceable by park²⁵

(Zanon et al., 2017)

Scer\GAL4^Tub.PU, Stoml2^GD16706 has wing phenotype, enhanceable by Scer\GAL4^Tub.PU/park^KK107919

(Zanon et al., 2017)

Scer\GAL4^Tub.PU, Stoml2^GD16706 has thorax phenotype, enhanceable by Scer\GAL4^Tub.PU/park^KK107919

(Zanon et al., 2017)

Suppressed by

Statement

Reference

Scer\GAL4^fln.IFM, Stoml2^GD16706 has wing phenotype, suppressible by Scer\GAL4^fln.IFM/park^UAS.cGa

(Zanon et al., 2017)

Df(2R)BSC695, Scer\GAL4^fln.IFM, Stoml2^GD16706 has wing phenotype, suppressible by park^UAS.cGa, Scer\GAL4^fln.IFM

(Zanon et al., 2017)

Df(2R)BSC770, Scer\GAL4^fln.IFM, Stoml2^GD16706 has wing phenotype, suppressible by park^UAS.cGa, Scer\GAL4^fln.IFM

(Zanon et al., 2017)

Enhancer of

Statement

Reference

Stoml2^GD16706/Scer\GAL4^Tub.PU is an enhancer of wing phenotype of Scer\GAL4^Tub.PU, park^KK107919

(Zanon et al., 2017)

Stoml2^GD16706/Scer\GAL4^Tub.PU is an enhancer of thorax phenotype of Scer\GAL4^Tub.PU, park^KK107919

(Zanon et al., 2017)

Scer\GAL4^ple.PU/Stoml2^GD16706 is an enhancer of dopaminergic PPL1 neuron phenotype of park²⁵

(Zanon et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The wing posture defects, the thorax indentations, but not the decreased flight performance or the decreased progeny, induced by the expression of either park^KK107919 or Stoml2^GD16706 under the control of Scer\GAL4^Tub.PU become exacerbated upon their co-expression.

Individuals both mutant for park²⁵ and expressing Stoml2^GD16706 under the control of Scer\GAL4^ple.PU show a more severe decrease in the number of PPL1 neurons in the adult brain than either individual mutant condition.

The wing posture defects induced by the expression of Stoml2^GD16706 under the control of Scer\GAL4^fln.IFM alone, or in combination with heterozygosity for either Df(2R)BSC695 or Df(2R)BSC770, are fully suppressed by the co-expression of park^UAS.cGa.

The decreased adult climbing capacity observed upon the expression of Stoml2^GD16706 under the control of Scer\GAL4^ple.PU alone, or in combination with heterozygosity for either Df(2R)BSC695 or Df(2R)BSC770, is fully suppressed by the co-expression of park^UAS.cGa.

(Zanon et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v48124

w¹¹¹⁸; P{GD16706}v48124/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

16706

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG2970^GD16706

Stoml2^GD16706

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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