FlyBase Allele Report: Dmel\Shmt[GD8851]

General Information

Symbol

Dmel\Shmt^GD8851

Species

D. melanogaster

Name

FlyBase ID

FBal0202474

Feature type

allele

Associated gene

Dmel\Shmt

Associated Insertion(s)

Carried in Construct

P{GD8851}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD8851}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD8851

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA

(Pilesi et al., 2023)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Parkinson's disease

modeled by park²⁵

(Celardo et al., 2017)

modeled by Pink1^B9

(Celardo et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Scer\GAL4^da.PU

(Celardo et al., 2017)

partially lethal - majority die, with Scer\GAL4^da.PU

(Celardo et al., 2017)

short lived, with Scer\GAL4^da.PU

(Celardo et al., 2017)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

chromosome | larval stage, with Scer\GAL4^Act.PU

(Pilesi et al., 2023)

larval brain | larval stage, with Scer\GAL4^Act.PU

(Pilesi et al., 2023)

larval brain | larval stage, with Scer\GAL4^elav.PU

(Celardo et al., 2017)

mitochondrion | larval stage, with Scer\GAL4^elav.PU

(Celardo et al., 2017)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Flies expressing Shmt2^GD8851 under the control of Scer\GAL4^da.PU display severely reduced eclosion rate compared to controls, the adults that manage to eclose show much decreased lifespan and significant climbing ability defects. Scer\GAL4^elav.PU-driven expression leads to fragmented mitochondrial network in larval neural tissue with decreased mean mitochondrial length.

(Celardo et al., 2017)

Adults expressing CG3011^GD8851 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 10-20% or 40-50% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 0% or 20-30% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

8851

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^da.PU/Shmt^GD8851 is an enhancer of visible | adult stage phenotype of Pink1^B9

(Celardo et al., 2017)

Scer\GAL4^da.PU/Shmt^GD8851 is an enhancer of partially lethal phenotype of park²⁵

(Celardo et al., 2017)

NOT Enhancer of

Statement

Reference

Scer\GAL4^da.PU/Shmt^GD8851 is a non-enhancer of partially lethal phenotype of Pink1^B9

(Celardo et al., 2017)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^da.PU/Shmt^GD8851 is an enhancer of adult thorax phenotype of Pink1^B9

(Celardo et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The lethality and dented thorax phenotype of Pink1^B9 mutants is enhanced upon Scer\GAL4^da.PU-driven expression of Shmt2^GD8851. A strong increase in lethality of park²⁵ mutants is also seen upon expression of either Shmt2^GD8851 (hardly any adults can be recovered).

(Celardo et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v19206