Allele Dmel\Slimp^GD10175

General Information
Symbol	Dmel\Slimp^GD10175	Species	D. melanogaster
Name		FlyBase ID	FBal0202745
Feature type	allele	Associated gene	Dmel\Slimp

Map ( GBrowse )
Allele class
Mutagen	in vitro construct - RNAi, in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Dickson et al., 2007.7.18) in vitro construct - RNAi (Dickson et al., 2007.7.18)
Mutations Mapped to the Genome

Type Location Additional Notes References RNAi reagent :1..370
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an inverted repeat. (Dickson et al., 2007.7.18)
Carried in construct	P{GD10175} (Dickson et al., 2007.7.18, Guitart et al., 2010, Mummery-Widmer et al., 2009)
Associated Sequence Features	dsRNA-GD10175
Cytology
Phenotypic Data
Phenotypic Class
	decreased cell number, with Scer\GAL4^ptc-559.1 (Guitart et al., 2010) lethal \| heat sensitive, with Scer\GAL4^Act5C.PU (Guitart et al., 2010) viable, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009)
Phenotype Manifest In
	anterior crossvein \| cell autonomous, with Scer\GAL4^ptc-559.1 (Guitart et al., 2010) mitochondrion, with Scer\GAL4^Act5C.PU (Guitart et al., 2010) wing cell \| cell autonomous, with Scer\GAL4^ptc-559.1 (Guitart et al., 2010)
Detailed Description
	Statement Reference Scer\GAL4[Act5C.PU]-mediated expression of Slimp[GD10175] significantly reduces viability: only 12% of flies emerge. Lethality is increased at higher temperatures. Lethality is significantly suppressed by addition of an anti-oxidant mix in the diet. Scer\GAL4[ptc-559.1]-mediated expression of Slimp[GD10175] results in a narrowing of the region flanked by longitudinal veins L3 and L4 in the adult wing. This is caused by a reduction in the number of cells and not a reduction in cell size. The anterior cross vein is reduced/lost in some cases. The relative amount of mitochondrial DNA in Scer\GAL4[Act5C.PU], Slimp[GD10175] larvae is increased compared to controls. Scer\GAL4[Act5C.PU]-mediated expression of Slimp[GD10175] does not reduce mitochondrial respiratory values (normalized by mitochondrial density). (Guitart et al., 2010)
External Data
Linkouts	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 10175
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Other
Statement Reference Dcr-2^Scer\UAS.cDa, Scer\GAL4^nub-AC-62, Slimp^GD10175 has wing phenotype (Guitart et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference Scer\GAL4[nub-AC-62]-mediated co-expression of Slimp[GD10175] and Dcr-2[Scer\UAS.cDa] results in failure of the wing to unfold, though the overall structure of the wing is not compromised. (Guitart et al., 2010)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 2 )
VDRC	v33774 w¹¹¹⁸; P{GD10175}v33774 v33773 w¹¹¹⁸; P{GD10175}v33773/TM3
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts
	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 10175
Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	CG31133^GD10175 Slimp^GD10175
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Guitart et al., 2010, J. Biol. Chem. 285(49): 38157--38166 New aminoacyl-tRNA synthetase-like protein in insecta with an essential mitochondrial function. [FBrf0212542]
Supplementary material	Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]

Allele Dmel\SlimpGD10175

Allele Dmel\Slimp^GD10175