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General Information
Symbol
Dmel\chasGD7593
Species
D. melanogaster
Name
FlyBase ID
FBal0203374
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a dsRNA that targets both A and B isoforms of chas.

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Tendon cell projections connecting dorsolongitudinal muscles do not elongate at 28-32 hours after puparium formation in animals expressing chasGD7593 under the control of Scer\GAL4pnr-MD237, in contrast to controls.

Expression of chasGD7593 under the control of Scer\GAL4pnr-MD237 in the notum results in notum bristles and trichomes pointing to the midline and multiple trichomes per cell. Epidermal indentations are seen in most anterior notum regions. The anterior indentations are detected by 27-28 hours after puparium formation (APF) and are very obvious by 32 hours APF, coinciding with the attachment domains of the ventral dorsolongitudinal muscles.

Bristle and socket cells of the notum have rotated towards the midline more than in controls in animals expressing chasGD7593 under the control of Scer\GAL4pnr-MD237 at 32 hours after puparium formation.

Expression of chasGD7593 under the control of Scer\GAL4pnr-MD237 from 14 hours after puparium formation (APF) (using the temperature sensitive Scer\GAL80ts.αTub84B allele to limit repress expression before this stage) results in weak bristle orientation defects in the notum.

Adults expressing chasGD7593 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression under the control of Scer\GAL4pnr-MD237 results in bristle morphology defects on the notum in 0% or 20-30% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4pnr-MD237 results in planar polarity defects in the orientation of bristles of the notum in 20-30% or 60-70% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Enhancer of
Phenotype Manifest In
Enhanced by
Suppressed by
Enhancer of
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of jbugGD13033 and chasGD7593 under the control of Scer\GAL4pnr-MD237 results in enhanced indentations of the anterior notum epidermis and enhanced planar polarity defects compared to either single mutant.

Co-expression of jbugL.Scer\UAS suppresses the planar polarity defects and the anterior epidermal indentations in the notum which are caused by expression of chasGD7593 under the control of Scer\GAL4pnr-MD237.

Co-expression of chasGD7593 and zipDN.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4pnr-MD237 from 14 hours after puparium formation (using the temperature sensitive Scer\GAL80ts.αTub84B allele to limit repress expression before this stage) results in strong bristle orientation defects and epidermal indentations in the notum.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
CG32556GD7593
chasGD7593
Name Synonyms
Secondary FlyBase IDs
    References (6)