The expression of ATPsynβL^GD11634 under the control of Scer\GAL4^VP16.bam results in spermatocytes S5/6 with a significant increase in the number of centrosomes, as compared to controls.

5-week old flies expressing ATPsynβL^GD11634 under the control of Scer\GAL4^Hand.PU and subjected to ad libitum feeding (ARF) do not exhibit significantly improved heart period compared to controls, but arrhythmia is reduced.

Under rich media conditions, knockdown of CG5389 through expression of CG5389^GD11634 under the control of Scer\GAL4^da.G32 confers a 26% increase in mean survival compared to isogenic control flies. This longevity effect is abolished in dietary restricted conditions, with lifespan of CG5389 knockdown flies being shortened in dietary restriction conditions.

Adult-only knockdown of CG5389 through expression of CG5389^GD11634 under the control of Scer\GAL4^Switch.tub in dietary-restricted conditions exhibit greatly impaired longevity. Flies exposed to oligomycin, a specific inhibitor of CG5389m from the onset of adulthood do not show significant lifespan extension under dietary restriction conditions without shortening lifespan under rich media conditions.

Adults expressing ATPsynβL^GD11634 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Ubiquitous expression using Scer\GAL4^Switch.tub, induced during larval development and adulthood, prolongs life span in female flies and has variable effects on male life span. Female fertility is reduced. Flies do not show improved tolerance to paraquat poisoning or starvation.

Ubiquitous expression using Scer\GAL4^Switch.tub from the onset of adulthood only does not confer robust longevity effects in male or female flies. Female fertility is also unaffected.

Constitutive or adult only neuronal expression using Scer\GAL4^{elav.Switch.PO} has no robust longevity effects in female or male flies.