The expression of scribGD11666 under the control of Scer\GAL4ptc-559.1 induces invasive cell migration in the ptc domain along the anterior-posterior compartment boundary of the third instar larval wing disc.
Expressing scribGD11666 under the control of Scer\GAL4ptc.PU induces some cells at the wing disc A/P boundary to delaminate and migrate to the posterior compartment.
Expressing scribGD11666 at the anterior/posterior boundary of the third instar larval wing disc (under the control of Scer\GAL4ptc-559.1) leads to severe cell migration away from the ptc-positive domain.
Expression of scribGD11666 under the control of Scer\GAL4Hml.Δ does not lead to a significant difference in survival as compared to controls when larvae are infected with the nematode Heterorhabditis bacteriophora and its associated bacterium Photorhabdus luminescens.
Expression of scribGD11666 under the control of Scer\GAL4ple.PF or Scer\GAL4GMR13F02 (in combination with a Dicer-2 transgene to enhance RNAi efficiency) results in enhanced memory after conditioning in an olfactory conditioning assay.
scribGD11666-expressing clones in the wing or eye disc do not survive.
When scribGD11666 is expressed along the anterior/posterior (A/P) compartment boundary of wing imaginal discs under the control of Scer\GAL4ptc.PU, cells delaminate and migrate away from the (A/P boundary).
Down-regulation of scrib along the anterior/posterior compartment boundary, through expression of scribGD11666 under the control of Scer\GAL4ptc-559.1, produces a JNK-dependent invasion-like phenotype, in which cells delaminate and migrate away posteriorly.
Adults expressing scribGD11666 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Depending on the insertion line used, expression under the control of Scer\GAL4Mef2.PR can result in viable flies or late larval lethality.