FlyBase Allele Report: Dmel\scrib[GD11666]

General Information

Symbol

Dmel\scrib^GD11666

Species

D. melanogaster

Name

FlyBase ID

FBal0204693

Feature type

allele

Associated gene

Dmel\scrib

Associated Insertion(s)

Carried in Construct

P{GD11666}

Also Known As

UAS-scrib-IR, UAS-scrib-RNAi

Key Links

Genomic Maps

GBrowse JBrowse

Help me choose

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

in vitro construct - RNAi

(Dickson et al., 2007.7.18)

in vitro construct - regulatory fusion

(Dickson et al., 2007.7.18)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{GD11666}

Cytology

Nature of the lesion

Statement

Reference

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

scrib

Associated Sequence Features

dsRNA-GD11666

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of carcinoma

CEA

(Ding et al., 2022, Sun et al., 2019, Zhang et al., 2019)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of carcinoma

is ameliorated by lic^GD7546

(Sun et al., 2019)

is ameliorated by lic^JF01433

(Sun et al., 2019)

is ameliorated by pan^JF02306

(Zhang et al., 2019)

is ameliorated by wg^GD5007

(Zhang et al., 2019)

is exacerbated by Toll-7^{C993Y.UAS.Tag:FLAG}

(Ding et al., 2022)

is ameliorated by Toll-7^GD14417

(Ding et al., 2022)

is ameliorated by Toll-7^HM05230

(Ding et al., 2022)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

anterior-posterior compartment boundary of the wing disc, with Scer\GAL4^ptc.PU

(Ma et al., 2014)

anterior-posterior compartment boundary of the wing disc | third instar larval stage, with Scer\GAL4^ptc-559.1

(Ding et al., 2022, Zhang et al., 2019)

anterior-posterior compartment boundary of the wing disc | third instar larval stage, with Scer\GAL4^ptc.PU

(Sun et al., 2019)

epithelial cell, with Scer\GAL4^ptc-559.1

(Ma et al., 2013)

Detailed Description

Statement

Reference

The expression of scrib^GD11666 under the control of Scer\GAL4^ptc-559.1 induces invasive cell migration in the ptc domain along the anterior-posterior compartment boundary of the third instar larval wing disc.

(Ding et al., 2022)

Expressing scrib^GD11666 under the control of Scer\GAL4^ptc.PU induces some cells at the wing disc A/P boundary to delaminate and migrate to the posterior compartment.

(Sun et al., 2019)

Expressing scrib^GD11666 at the anterior/posterior boundary of the third instar larval wing disc (under the control of Scer\GAL4^ptc-559.1) leads to severe cell migration away from the ptc-positive domain.

(Zhang et al., 2019)

Expression of scrib^GD11666 under the control of Scer\GAL4^Hml.Δ does not lead to a significant difference in survival as compared to controls when larvae are infected with the nematode Heterorhabditis bacteriophora and its associated bacterium Photorhabdus luminescens.

(Arefin et al., 2017)

Expression of scrib^GD11666 under the control of Scer\GAL4^ple.PF or Scer\GAL4^GMR13F02 (in combination with a Dicer-2 transgene to enhance RNAi efficiency) results in enhanced memory after conditioning in an olfactory conditioning assay.

(Cervantes-Sandoval et al., 2016)

scrib^GD11666-expressing clones in the wing or eye disc do not survive.

(Andersen et al., 2015)

When scrib^GD11666 is expressed along the anterior/posterior (A/P) compartment boundary of wing imaginal discs under the control of Scer\GAL4^ptc.PU, cells delaminate and migrate away from the (A/P boundary).

(Ma et al., 2014)

Down-regulation of scrib along the anterior/posterior compartment boundary, through expression of scrib^GD11666 under the control of Scer\GAL4^ptc-559.1, produces a JNK-dependent invasion-like phenotype, in which cells delaminate and migrate away posteriorly.

(Ma et al., 2013)

Adults expressing scrib^GD11666 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in viable flies or late larval lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 30-40% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

11666

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^ptc-559.1, scrib^GD11666 has abnormal cell migration | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^ptc-559.1/Toll-7^GD14417

(Ding et al., 2022)

Scer\GAL4^ptc-559.1, scrib^GD11666 has abnormal cell migration | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^ptc-559.1/Toll-7^HM05230

(Ding et al., 2022)

Scer\GAL4^ptc-559.1, scrib^GD11666 has abnormal cell migration | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^ptc-559.1/puc^UAS.cMa

(Ding et al., 2022, Zhang et al., 2019)

Scer\GAL4^ptc.PU, scrib^GD11666 has abnormal cell migration | third instar larval stage phenotype, suppressible by lic^JF01433/Scer\GAL4^ptc.PU

(Sun et al., 2019)

Scer\GAL4^ptc.PU, scrib^GD11666 has abnormal cell migration | third instar larval stage phenotype, suppressible by puc^UAS.cUa/Scer\GAL4^ptc.PU

(Sun et al., 2019)

Scer\GAL4^ptc.PU, scrib^GD11666 has abnormal cell migration | third instar larval stage phenotype, suppressible by lic^GD7546/Scer\GAL4^ptc.PU

(Sun et al., 2019)

Scer\GAL4^ptc-559.1, scrib^GD11666 has abnormal cell migration | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^ptc-559.1/wg^GD5007

(Zhang et al., 2019)

Scer\GAL4^ptc-559.1, scrib^GD11666 has abnormal cell migration | third instar larval stage phenotype, suppressible | partially by pan^GD1506/Scer\GAL4^ptc-559.1

(Zhang et al., 2019)

Scer\GAL4^ptc-559.1, scrib^GD11666 has abnormal cell migration | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^ptc-559.1/wg^NIG.4889R

(Zhang et al., 2019)

Scer\GAL4^ptc-559.1, scrib^GD11666 has abnormal cell migration | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^ptc-559.1/pan^JF02306

(Zhang et al., 2019)

Scer\GAL4^ptc-559.1, scrib^GD11666 has abnormal cell migration | third instar larval stage phenotype, suppressible | partially by bsk^DN.UAS/Scer\GAL4^ptc-559.1

(Zhang et al., 2019)

Scer\GAL4^Act5C.PP, scrib^GD11666 has FlyBase_internaltransgene_features:cell lethal:cell lethal | somatic clone phenotype, suppressible by grnd^KK109939/Scer\GAL4^Act5C.PP

(Andersen et al., 2015)

Scer\GAL4^ptc-559.1, scrib^GD11666 has neoplasia phenotype, suppressible by Scer\GAL4^ptc-559.1/Uev1A^GD6650

(Ma et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^ptc-559.1, scrib^GD11666 has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^ptc-559.1/Toll-7^GD14417

(Ding et al., 2022)

Scer\GAL4^ptc-559.1, scrib^GD11666 has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^ptc-559.1/Toll-7^HM05230

(Ding et al., 2022)

Scer\GAL4^ptc-559.1, scrib^GD11666 has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^ptc-559.1/puc^UAS.cMa

(Ding et al., 2022, Zhang et al., 2019)

Scer\GAL4^ptc.PU, scrib^GD11666 has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, suppressible by puc^UAS.cUa/Scer\GAL4^ptc.PU

(Sun et al., 2019)

Scer\GAL4^ptc.PU, scrib^GD11666 has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, suppressible by lic^GD7546/Scer\GAL4^ptc.PU

(Sun et al., 2019)

Scer\GAL4^ptc.PU, scrib^GD11666 has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, suppressible by lic^JF01433/Scer\GAL4^ptc.PU

(Sun et al., 2019)

Scer\GAL4^ptc-559.1, scrib^GD11666 has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^ptc-559.1/wg^GD5007

(Zhang et al., 2019)

Scer\GAL4^ptc-559.1, scrib^GD11666 has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, suppressible | partially by pan^GD1506/Scer\GAL4^ptc-559.1

(Zhang et al., 2019)

Scer\GAL4^ptc-559.1, scrib^GD11666 has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^ptc-559.1/wg^NIG.4889R

(Zhang et al., 2019)

Scer\GAL4^ptc-559.1, scrib^GD11666 has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^ptc-559.1/pan^JF02306

(Zhang et al., 2019)

Scer\GAL4^ptc-559.1, scrib^GD11666 has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, suppressible | partially by bsk^DN.UAS/Scer\GAL4^ptc-559.1

(Zhang et al., 2019)

Scer\GAL4^ptc.PU, scrib^GD11666 has anterior-posterior compartment boundary of the wing disc phenotype, suppressible by ben^GD1387/Scer\GAL4^ptc.PU

(Ma et al., 2014)

Scer\GAL4^ptc.PU, scrib^GD11666 has anterior-posterior compartment boundary of the wing disc phenotype, suppressible by ben[+]/ben¹

(Ma et al., 2014)

Scer\GAL4^ptc-559.1, scrib^GD11666 has epithelial cell phenotype, suppressible by Scer\GAL4^ptc-559.1/Uev1A^GD6650

(Ma et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of ben^GD1387 suppresses the cell migration seen when scrib^GD11666 is expressed along the anterior/posterior (A/P) compartment boundary of wing imaginal discs under the control of Scer\GAL4^ptc.PU.

One copy of ben¹ suppresses the cell migration seen when scrib^GD11666 is expressed along the anterior/posterior (A/P) compartment boundary of wing imaginal discs under the control of Scer\GAL4^ptc.PU.

(Ma et al., 2014)

Loss of Uev1A, through co-expression of Uev1A^GD6650, significantly suppresses the epithelial migration phenotype found in flies expressing scrib^GD11666 under the control of Scer\GAL4^ptc-559.1.

(Ma et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

VDRC

v27430