UASt regulatory sequences drive expression of an inverted repeat.
The expression of scribGD11666 under the control of Scer\GAL4ptc-559.1 induces invasive cell migration in the ptc domain along the anterior-posterior compartment boundary of the third instar larval wing disc.
Expressing scribGD11666 under the control of Scer\GAL4ptc.PU induces some cells at the wing disc A/P boundary to delaminate and migrate to the posterior compartment.
Expressing scribGD11666 at the anterior/posterior boundary of the third instar larval wing disc (under the control of Scer\GAL4ptc-559.1) leads to severe cell migration away from the ptc-positive domain.
Expression of scribGD11666 under the control of Scer\GAL4Hml.Δ does not lead to a significant difference in survival as compared to controls when larvae are infected with the nematode Heterorhabditis bacteriophora and its associated bacterium Photorhabdus luminescens.
Expression of scribGD11666 under the control of Scer\GAL4ple.PF or Scer\GAL4GMR13F02 (in combination with a Dicer-2 transgene to enhance RNAi efficiency) results in enhanced memory after conditioning in an olfactory conditioning assay.
scribGD11666-expressing clones in the wing or eye disc do not survive.
When scribGD11666 is expressed along the anterior/posterior (A/P) compartment boundary of wing imaginal discs under the control of Scer\GAL4ptc.PU, cells delaminate and migrate away from the (A/P boundary).
Down-regulation of scrib along the anterior/posterior compartment boundary, through expression of scribGD11666 under the control of Scer\GAL4ptc-559.1, produces a JNK-dependent invasion-like phenotype, in which cells delaminate and migrate away posteriorly.
Adults expressing scribGD11666 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Depending on the insertion line used, expression under the control of Scer\GAL4Mef2.PR can result in viable flies or late larval lethality.
Expression under the control of Scer\GAL4pnr-MD237 may result in lethality, depending on the insertion line used.
Expression under the control of Scer\GAL4pnr-MD237 results in the absence of 30-40% of the Scer\GAL4pnr-MD237-expressing area of the notum.
Scer\GAL4ptc-559.1, scribGD11666 has abnormal cell migration | third instar larval stage phenotype, suppressible | partially by Scer\GAL4ptc-559.1/Toll-7GD14417
Scer\GAL4ptc-559.1, scribGD11666 has abnormal cell migration | third instar larval stage phenotype, suppressible | partially by Scer\GAL4ptc-559.1/Toll-7HM05230
Scer\GAL4ptc-559.1, scribGD11666 has abnormal cell migration | third instar larval stage phenotype, suppressible | partially by Scer\GAL4ptc-559.1/pucUAS.cMa
Scer\GAL4ptc.PU, scribGD11666 has abnormal cell migration | third instar larval stage phenotype, suppressible by licJF01433/Scer\GAL4ptc.PU
Scer\GAL4ptc.PU, scribGD11666 has abnormal cell migration | third instar larval stage phenotype, suppressible by pucUAS.cUa/Scer\GAL4ptc.PU
Scer\GAL4ptc.PU, scribGD11666 has abnormal cell migration | third instar larval stage phenotype, suppressible by licGD7546/Scer\GAL4ptc.PU
Scer\GAL4ptc-559.1, scribGD11666 has abnormal cell migration | third instar larval stage phenotype, suppressible | partially by Scer\GAL4ptc-559.1/wgGD5007
Scer\GAL4ptc-559.1, scribGD11666 has abnormal cell migration | third instar larval stage phenotype, suppressible | partially by panGD1506/Scer\GAL4ptc-559.1
Scer\GAL4ptc-559.1, scribGD11666 has abnormal cell migration | third instar larval stage phenotype, suppressible | partially by Scer\GAL4ptc-559.1/wgNIG.4889R
Scer\GAL4ptc-559.1, scribGD11666 has abnormal cell migration | third instar larval stage phenotype, suppressible | partially by Scer\GAL4ptc-559.1/panJF02306
Scer\GAL4ptc-559.1, scribGD11666 has abnormal cell migration | third instar larval stage phenotype, suppressible | partially by bskDN.UAS/Scer\GAL4ptc-559.1
Scer\GAL4Act5C.PP, scribGD11666 has FlyBase_internaltransgene_features:cell lethal:cell lethal | somatic clone phenotype, suppressible by grndKK109939/Scer\GAL4Act5C.PP
Scer\GAL4ptc-559.1, scribGD11666 has neoplasia phenotype, suppressible by Scer\GAL4ptc-559.1/Uev1AGD6650
Scer\GAL4ptc-559.1, scribGD11666 has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, suppressible | partially by Scer\GAL4ptc-559.1/Toll-7GD14417
Scer\GAL4ptc-559.1, scribGD11666 has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, suppressible | partially by Scer\GAL4ptc-559.1/Toll-7HM05230
Scer\GAL4ptc-559.1, scribGD11666 has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, suppressible | partially by Scer\GAL4ptc-559.1/pucUAS.cMa
Scer\GAL4ptc.PU, scribGD11666 has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, suppressible by pucUAS.cUa/Scer\GAL4ptc.PU
Scer\GAL4ptc.PU, scribGD11666 has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, suppressible by licGD7546/Scer\GAL4ptc.PU
Scer\GAL4ptc.PU, scribGD11666 has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, suppressible by licJF01433/Scer\GAL4ptc.PU
Scer\GAL4ptc-559.1, scribGD11666 has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, suppressible | partially by Scer\GAL4ptc-559.1/wgGD5007
Scer\GAL4ptc-559.1, scribGD11666 has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, suppressible | partially by panGD1506/Scer\GAL4ptc-559.1
Scer\GAL4ptc-559.1, scribGD11666 has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, suppressible | partially by Scer\GAL4ptc-559.1/wgNIG.4889R
Scer\GAL4ptc-559.1, scribGD11666 has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, suppressible | partially by Scer\GAL4ptc-559.1/panJF02306
Scer\GAL4ptc-559.1, scribGD11666 has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, suppressible | partially by bskDN.UAS/Scer\GAL4ptc-559.1
Scer\GAL4ptc.PU, scribGD11666 has anterior-posterior compartment boundary of the wing disc phenotype, suppressible by benGD1387/Scer\GAL4ptc.PU
Scer\GAL4ptc.PU, scribGD11666 has anterior-posterior compartment boundary of the wing disc phenotype, suppressible by ben[+]/ben1
Scer\GAL4ptc-559.1, scribGD11666 has epithelial cell phenotype, suppressible by Scer\GAL4ptc-559.1/Uev1AGD6650
Expression of benGD1387 suppresses the cell migration seen when scribGD11666 is expressed along the anterior/posterior (A/P) compartment boundary of wing imaginal discs under the control of Scer\GAL4ptc.PU.
One copy of ben1 suppresses the cell migration seen when scribGD11666 is expressed along the anterior/posterior (A/P) compartment boundary of wing imaginal discs under the control of Scer\GAL4ptc.PU.
Loss of Uev1A, through co-expression of Uev1AGD6650, significantly suppresses the epithelial migration phenotype found in flies expressing scribGD11666 under the control of Scer\GAL4ptc-559.1.