FlyBase Allele Report: Dmel\CCT4[GD11684]

General Information

Symbol

Dmel\CCT4^GD11684

Species

D. melanogaster

Name

FlyBase ID

FBal0204714

Feature type

allele

Associated gene

Dmel\CCT4

Associated Insertion(s)

Carried in Construct

P{GD11684}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD11684}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD11684

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of intrinsic cardiomyopathy

CEA

(Melkani et al., 2017)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

abnormal neuroanatomy | larval stage, with Scer\GAL4^wor.PA

(Kuang et al., 2014)

lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

lethal, with Scer\GAL4^Act5C.PI

(Raut et al., 2017)

lethal, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

lethal, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

lethal, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

lethal - all die before end of P-stage, with Scer\GAL4^ey.PH

(Raut et al., 2017)

lethal - all die during embryonic stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

short lived, with Scer\GAL4^HCH.Hand

(Melkani et al., 2017)

short lived, with Scer\GAL4^tin.CΔ4

(Melkani et al., 2017)

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

adult heart, with Scer\GAL4^HCH.Hand

(Melkani et al., 2017)

adult heart | progressive, with Scer\GAL4^tin.CΔ4

(Melkani et al., 2017)

cardiac myofibril | adult stage, with Scer\GAL4^tin.CΔ4

(Melkani et al., 2017)

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

neuroblast | larval stage, with Scer\GAL4^wor.PA

(Kuang et al., 2014)

neuromuscular junction | third instar larval stage, with Scer\GAL4^elav-C155

(Raut et al., 2017)

sarcomere, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

terminal bouton | third instar larval stage, with Scer\GAL4^elav-C155

(Raut et al., 2017)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Z disc, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

Detailed Description

Statement

Reference

The expression of CCT4^GD11684 under the control of either Scer\GAL4^tin.CΔ4 or Scer\GAL4^HCH.Hand leads to a severe decrease in lifespan and leads to a severe decrease in lifespan and leads to a severe decrease in lifespan and leads to near or fully penetrant cardiac defects, respectively, namely morphological defects, non-beating heart segments and non-beating hearts, as compared to controls.

The expression of CCT4^GD11684 under the control of Scer\GAL4^tin.CΔ4 also leads to progressive cardiac dysfunction, as shown by significant increases in heart period, arrhythmia, and diastolic and systolic intervals, and by significant decreases in fractional shortening, and diastolic and systolic diameter, as compared to controls. These hearts show a severe and progressive disorganization in the actin- and myosin-containing myofibrils, as compared to controls.

(Melkani et al., 2017)

The expression of CCT4^GD11684 under the control of Scer\GAL4^elav-C155 leads to the neuromuscular junctions of third instar larval muscles 6/7 exhibiting decreases in the size and number of terminal boutons, which also present a significant decrease in microtubule loops, as compared to controls.

(Raut et al., 2017)

Expression of CG5525^GD11684 under the control of Scer\GAL4^wor.PA induces neuroblast loss in larval brains. Many of the remaining neuroblasts exhibit necrosis markers, including aggregation of mitochondria, elevated levels of calcium ions and the presence of ubiquitin-conjugated protein aggregates.

(Kuang et al., 2014)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in loss of neuroblasts in the larval brain without any change in size of the remaining neuroblasts.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in mild defects in the shape of neuroblasts in the larval brain.

(Neumüller et al., 2011)

Adults expressing CG5525^GD11684 under the control of the cardioblast-specific Scer\GAL4^tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in adult/pharate lethality or early larval lethality.

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal larval body wall muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in sarcomeres with reduced Z-lines in the larval body wall muscles.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in lethality before the pupal stage, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 20-30% of the Scer\GAL4^pnr-MD237 expression domain.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development