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General Information
Symbol
Dmel\Vps35GD11710
Species
D. melanogaster
Name
FlyBase ID
FBal0204753
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dVPS35 RNAi
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UASt regulatory sequences drive expression of an inverted repeat.
Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
The expression of Vps35GD11710 under the control of either Scer\GAL4Toll-6-D42 or Scer\GAL4Mhc.PK leads to significant increases both in the number of type Ib synaptic boutons and in the miniature excitatory junction potential amplitude at the third instar larval neuromuscular junction, as compared to controls. Expression under the control of Scer\GAL4ple.PF leads to a significant decrease in spontaneous synaptic activity in adult dendritic arborizing neurons and to defective sleep behavior, shown by reciprocal significant increase in sleep bouts during the light period and significant decrease in sleep bouts during the dark period, as compared to controls.
Expression of Vps35GD11710 under the control of Scer\GAL4GMR.PS does not result in any obvious eye phenotype, but does lead to a progressive defect in climbing ability, as compared to controls.
Adults expressing Vps35GD11710 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) can show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies, depending on the particular P{GD11710} insertion line used.
Expression under the control of Scer\GAL4pnr-MD237 may result in semi-lethality, depending on the insertion line used. Expression under the control of Scer\GAL4pnr-MD237 results in the absence of 0% or 10-20% of the Scer\GAL4pnr-MD237-expressing area of the notum, depending on the insertion line used.
External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Co-expression of Vps35GD11710 and Hsap\SNCAScer\UAS.cFa under the control of Scer\GAL4GMR.PS leads to a small reduction in size of each ommatidium along with loss of interommatidial bristles, as compared to controls; and leads to a more severe progressive climbing defect, as compared to expression of either single allele alone.
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
CG5625GD11710
Dvps35RNAi
Vps35GD11710
Name Synonyms
Secondary FlyBase IDs
    References (11)