FlyBase Allele Report: Dmel\Mitofilin[GD4642]

General Information

Symbol

Dmel\Mitofilin^GD4642

Species

D. melanogaster

Name

FlyBase ID

FBal0205086

Feature type

allele

Associated gene

Dmel\Mitofilin

Associated Insertion(s)

Carried in Construct

P{GD4642}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD4642}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD4642

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates frontotemporal dementia and/or amyotrophic lateral sclerosis 2

modeled by Chchd2^S81L.UAS

(Baek et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

axon | third instar larval stage, with Scer\GAL4^CCAP.PP

(Tsai et al., 2017)

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing, with Scer\GAL4^Mhc.PU

(Tsai et al., 2017)

Detailed Description

Statement

Reference

The expression of Mitofilin^GD4642 under the control of either Scer\GAL4^Mhc.PU or Scer\GAL4^elav.PU leads to a significant decrease in flight ability, but does not impair the climbing ability, as compared to controls. Expression under the control of Scer\GAL4^Mhc.PU, but not Scer\GAL4^elav.PU, leads a 'drooped wing posture, as compared to controls.

Third instar larvae expressing Mitofilin^GD4642 under the control of Scer\GAL4^CCAP.PP show a severe decrease in both anterograde and retrograde axonal transport of mitochondria, as compared to controls.

The expression of Mitofilin^GD4642 under the control of Scer\GAL4^Act.PU results in the third instar larval neuromuscular junctions not showing any obvious defects in the number of synaptic branches, or number and size of synaptic boutons, nor in mitochondrial membrane potential (assessed by tetramethylrhodamine) or in vesicle loading (in FM1-43 loading assay) in the synaptic boutons, as compared to controls. Expression of under the control of either Scer\GAL4^Mhc.PU or Scer\GAL4^elav.PU also not lead to any significant changes in the number of synaptic boutons or in vesicle loading (in FM1-43 loading assay) in those synaptic boutons, as compared to controls.

(Tsai et al., 2017)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in a reduction in the average size of neuroblasts together with shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

(Neumüller et al., 2011)

Adults expressing Mitofilin^GD4642 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of Mitofilin^GD4642 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or complete lethality, depending on the particular P{GD4642} insertion line used.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 10-20% of the Scer\GAL4^pnr-MD237 expression domain.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development