FlyBase Allele Report: Dmel\Dlg5[GD11943]

General Information

Symbol

Dmel\Dlg5^GD11943

Species

D. melanogaster

Name

FlyBase ID

FBal0205114

Feature type

allele

Associated gene

Dmel\Dlg5

Associated Insertion(s)

Carried in Construct

P{GD11943}

Also Known As

UAS-dlg5.RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD11943}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD11943

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

atrophy | pupal stage, with Scer\GAL4^Mef2.PR

(Kaya-Çopur et al., 2021)

decreased cell number | pupal stage, with Scer\GAL4^Mef2.PR

(Kaya-Çopur et al., 2021)

flightless, with Scer\GAL4^Mef2.PR

(Kaya-Çopur et al., 2021)

increased size | pupal stage, with Scer\GAL4^Mef2.PR

(Kaya-Çopur et al., 2021)

lethal - all die before end of P-stage, with Scer\GAL4^Tub.PU

(Reilly et al., 2015)

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

majority die during third instar larval stage, with Scer\GAL4^Tub.PU

(Reilly et al., 2015)

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Kaya-Çopur et al., 2021)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

border follicle cell, with Scer\GAL4^c306

(Aranjuez et al., 2012)

chaeta | increased number, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

egg chamber, with Scer\GAL4^C587

(Reilly et al., 2015)

egg chamber, with Scer\GAL4^Tub.PU

(Reilly et al., 2015)

indirect flight muscle cell | pupal stage, with Scer\GAL4^Mef2.PR

(Kaya-Çopur et al., 2021)

myotube | decreased number | pupal stage, with Scer\GAL4^Mef2.PR

(Kaya-Çopur et al., 2021)

myotube | pupal stage, with Scer\GAL4^Mef2.PR

(Kaya-Çopur et al., 2021)

nucleus | pupal stage, with Scer\GAL4^Mef2.PR

(Kaya-Çopur et al., 2021)

polar follicle cell | increased number, with Scer\GAL4^C587

(Reilly et al., 2015)

stalk follicle cell, with Scer\GAL4^C587

(Reilly et al., 2015)

stalk follicle cell, with Scer\GAL4^Tub.PU

(Reilly et al., 2015)

Detailed Description

Statement

Reference

The expression of Dlg5^GD11943 under the control of Scer\GAL4^Mef2.PR leads to complete indirect flight muscle atrophy during pupal development (after 32h APF), with fewer muscle fibers; fibers fail to compact at 32h APF. Expression from pupal stages onward (by the additional control of Gal80[ts[) leads to similar defects.

(Kaya-Çopur et al., 2021)

Flies expressing Dlg5^GD11943 under the control of Scer\GAL4^Tub.PU die at the third instar larval stage with approximately 2% of animals surviving into the pupal stage.

Conditional expression of Dlg5^GD11943 in somatic cells under the control of Scer\GAL4^Tub.PU (GAL80ts has been used to limit expression to the adult stage) results in stalk cell overgrowth. Ovarioles containing degenerating follicles with only nurse cell nuclei and surrounding follicle cells remaining are also observed.

(Reilly et al., 2015)

Expression of CG6509^GD11943 under the control of Scer\GAL4^c306 results in incomplete border cell migration in 26% of stage 10 egg chambers. 44% of stage 9 border cell clusters no longer have the normal compact shape but are dissociated or elongated instead.

(Aranjuez et al., 2012)

Adults expressing Dlg5^GD11943 under the control of the cardioblast-specific Scer\GAL4^tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

(Neely et al., 2010)

Adults expressing Dlg5^GD11943 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in late pupal lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in extra bristles on the notum in 20-30% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 20-30% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development