When fed on a low carbohydrate diet (0% sucrose supplement), no developmental delay is observed for Scer\GAL4^Cg.PA GlyS^GD12070 (plus Dcr-2^Scer\UAS.cDa) animals. In contrast, when fed a 10% or 20% sucrose supplemented diet, these animals exhibit a slight delay.

Expression of GlyS^GD12070 under the control of Scer\GAL4^elav.PLu leads to a decrease in the age-dependent accumulation of glycogen granules in the neurons of 31-day-old flies, and improves the climbing performance of 31-day-old, but not 5-day-old flies, as compared to age-matched controls, and expression of GlyS^GD12070 under the control of Scer\GAL4^elav.PLu (in combination with a Gal80[ts] transgene to restrict expression to the adult stage) leads to an increase in lifespan in males, but not females, as compared to controls.

Expression of GlyS^GD12070 under the control of Scer\GAL4^Mef2.PR is able to significantly reduce the size of autophagosomes in the muscles of chloroquine-treated larvae starved on low-nutrient food for 6 hours. The number of autophagosomes is not significantly changed compared to controls.

Adults expressing GlyS^GD12070 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of GlyS^GD12070 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or complete lethality, depending on the particular P{GD12070} insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 may result in lethality before the pupal stage, depending on the insertion line used.