Unlike controls, larvae expressing HHEX^GD4293 under the control of Scer\GAL4^αTub84B.PL are intolerant to high levels of dietary sucrose or 0.1M glucosamine, and fail to pupariate. Raising these animals on a high salt diet has a similar effect. However, these animals show a similar response to controls when raised on other diets, including diets containing hydrogen peroxide, silver nitrate, or high fat.

Expression of HHEX^GD4293 under the control of Scer\GAL4^tin.cBa (together with Scer\GAL4^Scer\UAS.cHa), Scer\GAL4^srp.Hemo, Scer\GAL4^sns.GCN, Scer\GAL4^byn-Gal4, Scer\GAL4^{Myo31DF-NP0001}, Scer\GAL4^Ilp2.PR or Scer\GAL4^Cg.PA does not affect the viability of larvae fed a high sucrose diet. In contrast, expression of HHEX^GD4293 via Scer\GAL4^esg-05730B or Scer\GAL4^Dot.PK does decrease survival to eclosion in a sugar-sensitive manner, and expression via Scer\GAL4^esg-05730B also decreases survival to pupariation in a sugar-sensitive manner.

Scer\GAL4^Cg.PA-mediated expression of HHEX^GD4293, together with Dcr-2^Scer\UAS.cDa, in the presence of high (1.0M) sucrose, is male lethal. Both larval and adult female flies are significantly smaller than controls; wandering third instar larvae are hyperglycemic; both adults and larvae have significantly lower triglyceride levels compared to controls.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in a reduction in the average size of neuroblasts together with shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

Adults expressing HHEX^GD4293 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in viable flies or early pupal lethality.

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in a colour difference between the central Scer\GAL4^pnr-MD237 expression domain of the notum and the surrounding lateral region in 0% or 10-20% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0-10% or 20-30% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 0-10% or 80-90% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.