At 6 hours after puparium formation, pupae expressing BI-1GD1660 (together with Dcr-2Scer\UAS.cDa) under the control of Scer\GAL4da.G32 exhibit faster elimination of salivary gland cells, which is associated with enhanced cell shrinkage and a decreased and disorganized action cytoskeleton, compared to controls.
Larvae expressing BI-1GD1660 under the control of Scer\GAL4da.G32 exhibit an augmentation in the number of autophagosomes and lysosomes in the fat body of fasting larvae.
A substantial extension of lifespan is observed during nutrient starvation when BI-1GD1660 is expressed under the control of Scer\GAL4da.G32.
Treatment of BI-1GD1660 Scer\GAL4da.G32 animals with tunicamycin (an ER stress agent) results in decreased survival, whereas the same treatment of control flies has no effect on lifespan.
Adults expressing BI-1GD1660 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.