FlyBase Allele Report: Dmel\Pfdn6[GD10604]

General Information

Symbol

Dmel\Pfdn6^GD10604

Species

D. melanogaster

Name

FlyBase ID

FBal0205597

Feature type

allele

Associated gene

Dmel\Pfdn6

Associated Insertion(s)

Carried in Construct

P{GD10604}

Genomic Maps

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Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

in vitro construct - RNAi

(Dickson et al., 2007.7.18)

in vitro construct - regulatory fusion

(Dickson et al., 2007.7.18)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{GD10604}

Cytology

Nature of the lesion

Statement

Reference

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Pfdn6

Associated Sequence Features

dsRNA-GD10604

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

ganglion mother cell | larval stage

(Neumüller et al., 2011)

type II neuroblast | increased number | third instar larval stage, with Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

(Zhang et al., 2016)

type I neuroblast | increased number | third instar larval stage, with Scer\GAL4^insc-Mz1407

(Zhang et al., 2016)

Detailed Description

Statement

Reference

Expression of Pfdn6^GD10604 under the control of either Scer\GAL4^insc-Mz1407 (with UAS-Dicer2 to enhance RNAi efficiency) or Scer\GAL4^wor.PA (together with Scer\GAL80^ase.PN) results in formation of ectopic neuroblasts (either type I or type II, respectively) in third instar larval brains.

(Zhang et al., 2016)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

(Neumüller et al., 2011)

Adults expressing CG7770^GD10604 under the control of the cardioblast-specific Scer\GAL4^tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

(Neely et al., 2010)

Adults expressing CG7770^GD10604 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of CG7770^GD10604 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or partial lethality, depending on the particular P{GD10604} insertion line used.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in late pupal lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in lethality (either before or at the pupal stage), depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development