Ubiquitous knockdown of ScoxGD898 driven by Scer\GAL4da.PU causes developmental arrest at the early third instar larval stage and larvae are smaller than wild type.
Flies with cardiac-specific knockdown of ScoxGD898 driven by Scer\GAL4tin.CΔ4 have a shorter lifespan (begin to die after 2 weeks) than controls, and show heart dysfunction (aggravated with age and dose-dependent). With one or two copies of ScoxGD898, diastolic interval is significantly increased and fractional shortening (heart tube contractility) is significantly decreased in both 1 or 2 week old flies, compared to controls; two copies significantly increases heart period (reduces heart rate) in 1 or 2 week old flies (one copy significantly increases heart period in 2 week old flies); two copies (but not one) significantly decreases both diastolic and systolic diameter in 1 or 2 week old flies. Cardiac-specific knockdown of ScoxGD898 (two copies) also leads to myofibrillar disorganization (including gaps between myofibrils, disorganization is strongest in the posterior half) and a narrowing of the heart tube (in 1 week old, and even more so 2 week old flies); conical chamber diameter is also significantly increased in 1 week old flies, compared to controls. Pupal heart structure appears normal. Scer\GAL4tin.CΔ4>ScoxGD898 hearts show increases in reactive oxygen species production; they also show signs of apoptosis (seen at 1 week and more so at 2 weeks old).
No apparent difference in morphology is observed with larvae and adults expressing Scer\GAL4Act.PU>ScoxGD898. However, the expression of ScoxGD898 is associated with lethality at the larval stage. The transgenic larvae survive for 12 days after hatching, but demonstrate reduced motility day by until death. The larvae stay within the medium with low mobility or no climbing of the walls of culture tubes, in clear contrast to wild-type flies.
Expression of ScoxGD898 under the control of Scer\GAL4pnr-MD237 results in a mild thoracic cleft, a reduction in the scutellum and thin bristles, typical of "Minute" mutations.
Expression of ScoxGD898 under the control of Scer\GAL4GMR.PFa has no effect on adult eye morphology.
Adults expressing ScoxGD898 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Depending on the insertion line used, expression under the control of Scer\GAL4Mef2.PR can result in early pupal lethality or late pupal lethality.