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General Information
Symbol
Dmel\ScoxGD898
Species
D. melanogaster
Name
FlyBase ID
FBal0206030
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Ubiquitous knockdown of ScoxGD898 driven by Scer\GAL4da.PU causes developmental arrest at the early third instar larval stage and larvae are smaller than wild type.

Flies with cardiac-specific knockdown of ScoxGD898 driven by Scer\GAL4tin.CΔ4 have a shorter lifespan (begin to die after 2 weeks) than controls, and show heart dysfunction (aggravated with age and dose-dependent). With one or two copies of ScoxGD898, diastolic interval is significantly increased and fractional shortening (heart tube contractility) is significantly decreased in both 1 or 2 week old flies, compared to controls; two copies significantly increases heart period (reduces heart rate) in 1 or 2 week old flies (one copy significantly increases heart period in 2 week old flies); two copies (but not one) significantly decreases both diastolic and systolic diameter in 1 or 2 week old flies. Cardiac-specific knockdown of ScoxGD898 (two copies) also leads to myofibrillar disorganization (including gaps between myofibrils, disorganization is strongest in the posterior half) and a narrowing of the heart tube (in 1 week old, and even more so 2 week old flies); conical chamber diameter is also significantly increased in 1 week old flies, compared to controls. Pupal heart structure appears normal. Scer\GAL4tin.CΔ4>ScoxGD898 hearts show increases in reactive oxygen species production; they also show signs of apoptosis (seen at 1 week and more so at 2 weeks old).

No apparent difference in morphology is observed with larvae and adults expressing Scer\GAL4Act.PU>ScoxGD898. However, the expression of ScoxGD898 is associated with lethality at the larval stage. The transgenic larvae survive for 12 days after hatching, but demonstrate reduced motility day by until death. The larvae stay within the medium with low mobility or no climbing of the walls of culture tubes, in clear contrast to wild-type flies.

Expression of ScoxGD898 under the control of Scer\GAL4pnr-MD237 results in a mild thoracic cleft, a reduction in the scutellum and thin bristles, typical of "Minute" mutations.

Expression of ScoxGD898 under the control of Scer\GAL4GMR.PFa has no effect on adult eye morphology.

Adults expressing ScoxGD898 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Depending on the insertion line used, expression under the control of Scer\GAL4Mef2.PR can result in early pupal lethality or late pupal lethality.

Expression under the control of Scer\GAL4pnr-MD237 results in bristle morphology defects on the notum in 100% of the Scer\GAL4pnr-MD237 expression domain.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT suppressed by
Statement
Reference
NOT Suppressor of
Phenotype Manifest In
Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
NOT Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Flies with co-expression of p53Scer\UAS.cUa along with Scer\GAL4tin.CΔ4>ScoxGD898 have no heartbeat and show significant heart degeneration, with complete loss of cardiac myofibrils.

Co-expression of rprScer\UAS.cUa increases myofibril disorganization seen in adult hearts of Scer\GAL4tin.CΔ4>ScoxGD898 flies (defects in 1 week old flies look similar to 2 week old Scer\GAL4tin.CΔ4>ScoxGD898 flies).

Co-expression of p53DN.Scer\UAS.cUa suppresses heart function and structure defects seen in Scer\GAL4tin.CΔ4>ScoxGD898 flies: diastolic interval, heart period and fractional shortening return to control levels and there are no obvious morphological heart defects (including correct alignment of cardiomyocyte myofibrils). Presence of p535A-1-4/p535A-1-4 suppresses heart structural defects seen in Scer\GAL4tin.CΔ4>ScoxGD898 flies: there are no obvious morphological heart defects (including correct alignment of cardiomyocyte myofibrils).

Co-expression of Diap1Scer\UAS.cUa rescues heart structural defects seen in Scer\GAL4tin.CΔ4>ScoxGD898 flies.

Co-expression of Ctr1AScer\UAS.T:Zzzz\FLAG has no effect on the "Minute"-like phenotype caused by expression of ScoxGD898 under the control of Scer\GAL4pnr-MD237.

Co-expression of ScoxGD898 does not suppress the cuticle hypopigmentation phenotype caused by expression of ATP7Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4pnr-MD237.

Xenogenetic Interactions
Statement
Reference

Co-expression of BacA\p35Scer\UAS.cUa rescues heart structural defects seen in Scer\GAL4tin.CΔ4>ScoxGD898 flies.

Complementation and Rescue Data
Comments

Co-expression of ScoxScer\UAS.T:Zzzz\FLAG rescues the "Minute"-like defects caused by expression of ScoxGD898 under the control of Scer\GAL4pnr-MD237.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
CG8885GD898
ScoxGD898
Name Synonyms
Secondary FlyBase IDs
    References (8)