Expression of Lrp4GD14320 results in the decreased synapse number (assessed by counting active zone marker puncta) in the VA1v olfactory receptor neurons (with the Scer\GAL4peb-GAL4 driver) or excitatory projection neurons terminating in the lateral horn (with Scer\GAL4Mz19) but not in Gad1-positive inhibitory neurons (with the Scer\GAL4Gad1.3.098 driver) or in the inhibitory projection neurons terminating in the lateral horn (with Scer\GAL4Mz699), while the neurite volume remains unaffected compared to controls in all cases.
Scer\GAL4peb-GAL4-driven expression of Lrp4GD14320 also results in complete loss of olfactory attraction of adult flies to an attractive odorant (apple cider vinegar) in a trap assay. This lack of olfactory attraction behavior is not due to general defects in locomotion.
Expression of CG8909GD14320 under the control of Scer\GAL4sal.BO (in combination with a Dicer-2 transgene to enhance RNAi efficiency) does not result in larval lethality at the L2/L3 transition.
Expression under the control of Scer\GAL41407 results in an increased number of GFP-positive aggregates within neuroblast lineage cells in the larval brain compared to controls (these aggregates may indicate cell death or a disturbance of internal cellular structures).
Adults expressing CG8909GD14320 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.