FlyBase Allele Report: Dmel\Lrp4[GD14320]

General Information

Symbol

Dmel\Lrp4^GD14320

Species

D. melanogaster

Name

FlyBase ID

FBal0206035

Feature type

allele

Associated gene

Dmel\Lrp4

Associated Insertion(s)

Carried in Construct

P{GD14320}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD14320}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD14320

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^Mz19

(Mosca et al., 2017)

abnormal neuroanatomy | adult stage, with Scer\GAL4^peb-GAL4

(Mosca et al., 2017)

abnormal smell perception | adult stage, with Scer\GAL4^peb-GAL4

(Mosca et al., 2017)

lethal, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

adult antennal lobe projection neuron, with Scer\GAL4^Mz19

(Mosca et al., 2017)

adult olfactory receptor neuron, with Scer\GAL4^peb-GAL4

(Mosca et al., 2017)

larval brain | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

presynaptic active zone | adult stage, with Scer\GAL4^Mz19

(Mosca et al., 2017)

presynaptic active zone | adult stage, with Scer\GAL4^peb-GAL4

(Mosca et al., 2017)

synapse | adult stage, with Scer\GAL4^Mz19

(Mosca et al., 2017)

synapse | adult stage, with Scer\GAL4^peb-GAL4

(Mosca et al., 2017)

Detailed Description

Statement

Reference

Expression of Lrp4^GD14320 results in the decreased synapse number (assessed by counting active zone marker puncta) in the VA1v olfactory receptor neurons (with the Scer\GAL4^peb-GAL4 driver) or excitatory projection neurons terminating in the lateral horn (with Scer\GAL4^Mz19) but not in Gad1-positive inhibitory neurons (with the Scer\GAL4^Gad1.3.098 driver) or in the inhibitory projection neurons terminating in the lateral horn (with Scer\GAL4^Mz699), while the neurite volume remains unaffected compared to controls in all cases.

Scer\GAL4^peb-GAL4-driven expression of Lrp4^GD14320 also results in complete loss of olfactory attraction of adult flies to an attractive odorant (apple cider vinegar) in a trap assay. This lack of olfactory attraction behavior is not due to general defects in locomotion.

(Mosca et al., 2017)

Expression of CG8909^GD14320 under the control of Scer\GAL4^sal.BO (in combination with a Dicer-2 transgene to enhance RNAi efficiency) does not result in larval lethality at the L2/L3 transition.

(Wicker-Thomas et al., 2015)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in an increased number of GFP-positive aggregates within neuroblast lineage cells in the larval brain compared to controls (these aggregates may indicate cell death or a disturbance of internal cellular structures).

(Neumüller et al., 2011)

Adults expressing CG8909^GD14320 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

14320

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Lrp4^GD14320, Scer\GAL4^peb-GAL4 has abnormal smell perception | adult stage phenotype, suppressible | partially by Srpk79D^UAS.Venus, Scer\GAL4^peb-GAL4

(Mosca et al., 2017)

Suppressor of

Statement

Reference

Scer\GAL80^ts.αTub84B, Lrp4^GD14320, Scer\GAL4^da.PU is a suppressor | heat sensitive of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4^da.PU, Scer\GAL80^ts.αTub84B, fzr^RNAi.UAS.WIZ

(Neuert et al., 2017)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The loss of olfactory attraction behavior in response to an attractive odorant (apple cider vinegar) in adult flies expressing Lrp4^GD14320 under the control of Scer\GAL4^peb-GAL4 is ameliorated by co-expression of Srpk79D^{Scer\UAS.T:Avic\GFP-YFP.Venus}.

(Mosca et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v29900