Adults expressing ND-20^GD13310 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies.

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in early pupal lethality or late larval lethality.

Ubiquitous expression using Scer\GAL4^Switch.tub, induced during larval development and adulthood, prolongs life span in female flies and has variable effects on male life span. Female fertility is reduced. Flies also show improved tolerance to paraquat poisoning, but do not show improved starvation resistance. High level induction causes larval lethality.

Ubiquitous expression using Scer\GAL4^Switch.tub from the onset of adulthood only also increases female life span and has variable effects on males. Female fertility is also reduced.

Constitutive or adult only neuronal expression using Scer\GAL4^{elav.Switch.PO} increases mean life span in females and has variable effects in males. Adult only neuronal expression does not significantly affect female fertility.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 40-50% or 80-90% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 10-20% or 60-70% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.