UASt regulatory sequences drive expression of an inverted repeat.
Expression of EogtGD5084 under the control of Scer\GAL4tub.PU at 30[o]C is late pupal lethal. 94% of animals expressing EogtGD5084 under the control of Scer\GAL4Act.PU at 30[o]C hatch, while only 37% hatch if Dcr-2Scer\UAS.cDa is also present (to enhance RNAi).
Expression of EogtGD5084 under the control of Scer\GAL4en.PU in the presence of Dcr-2Scer\UAS.cDa results in wing blistering specifically within the posterior compartment. This phenotype has almost complete penetrance at 27[o]C, and is not seen at 22.5[o]C. Eogtex10/+ enhances the phenotype, so that wing blistering with a penetrance of up to 30% is seen at 22.5[o]C.
Adults expressing EogtGD5084 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, enhanceable by dpylv1/dp[+]
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, enhanceable by dpyolvR/dp[+]
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, enhanceable by wb[+]/wbSF11
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, enhanceable by pyd3Lb5/Adcb-1
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, non-enhanceable by dpyv/e(dpyv)1
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, non-enhanceable by pio[+]/pio2R-16
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, non-enhanceable by pio[+]/pioMB03570
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, non-enhanceable by pot[+]/pote04564
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, non-enhanceable by mys1/mys[+]
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, suppressible | partially by crbj1B5/crb[+]
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, suppressible | partially by NAx-16/N[+]
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, suppressible | partially by N[+]/NAx-E2
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, suppressible | partially by NAx-9/N[+]
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, suppressible | partially by Nspl-1/N[+]
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, suppressible | partially by Df(1)N-264-105/+
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, suppressible | partially by N[+]/N55e11
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, suppressible | partially by Df(3R)ED6237/+
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, suppressible | partially by Df(3R)ED5942/+
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, suppressible | partially by Df(3L)ri-79c/+
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, suppressible | partially by Df(2L)TE35BC-4/+
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, suppressible | partially by Df(2R)BSC383/+
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, suppressible | partially by DeltaRevF10/Dl[+]
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, suppressible | partially by Ser[+]/SerRX106
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, suppressible | partially by PsnI2/Psn[+]
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, suppressible | partially by Psn227/Psn[+]
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, suppressible | partially by Su(H)[+]/Su(H)2
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, suppressible | partially by mam2/mam[+]
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, suppressible | partially by Df(1)N-81k1/+
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, suppressible | partially by N+t40
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, suppressible | partially by Dp(1;2)51b
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, suppressible | temperature conditional by r9
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, suppressible by rcSa/In(1)r70b
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, suppressible | partially by rSu(b).cSa/In(1)r70b
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, suppressible by Dhod8
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, suppressible by r-lK2
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, suppressible | partially by r-le01755
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, suppressible | partially by e(r)G926
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, suppressible | partially by pyd3+t3.8/pyd3Lb10/Adcb-1
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, suppressible by Adcb-1
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, suppressible by Hsap\EOGTTub.PM
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, non-suppressible by pio[+]/pio2R-16
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, non-suppressible by pio[+]/pioMB03570
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, non-suppressible by dpyv/e(dpyv)1
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, non-suppressible by pot[+]/pote04564
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, non-suppressible by mys1/mys[+]
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has visible | heat sensitive phenotype, non-suppressible by Mmus\Pomgnt2Tub.PM
Adcb-1, EogtGD5084, su(r)1 has lethal phenotype
Df(3R)noi-B, EogtGD5084 has lethal phenotype
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, enhanceable by wb[+]/wbSF11
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, enhanceable by dpy[+]/dpylv1
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, enhanceable by dpy[+]/dpyolvR
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, enhanceable by pyd3Lb5/Adcb-1
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, non-enhanceable by dpyv/e(dpyv)1
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, non-enhanceable by pio[+]/pio2R-16
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, non-enhanceable by pio[+]/pioMB03570
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, non-enhanceable by pot[+]/pote04564
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, non-enhanceable by mys1/mys[+]
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, suppressible | partially by Df(2R)BSC383/+
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, suppressible by rcSa/In(1)r70b
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, suppressible | partially by pyd3+t3.8/pyd3Lb10
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, suppressible | temperature conditional by r9
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, suppressible | partially by N+t40
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, suppressible | partially by Ser[+]/SerRX106
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, suppressible | partially by Nspl-1/N[+]
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, suppressible | partially by N[+]/NAx-E2
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, suppressible | partially by Df(3R)ED6237/+
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, suppressible | partially by rSu(b).cSa/In(1)r70b
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, suppressible | partially by PsnI2/Psn[+]
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, suppressible by Adcb-1
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, suppressible | partially by Df(2L)TE35BC-4/+
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, suppressible | partially by Df(1)N-81k1/+
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, suppressible | partially by Psn227/Psn[+]
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, suppressible | partially by Dp(1;2)51b
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, suppressible | partially by DeltaRevF10/Dl[+]
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, suppressible | partially by Df(1)N-264-105/+
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, suppressible | partially by Su(H)[+]/Su(H)2
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, suppressible by r-lK2
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, suppressible by Dhod8
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, suppressible | partially by Df(3R)ED5942/+
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, suppressible | partially by Df(3L)ri-79c/+
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, suppressible | partially by NAx-9/N[+]
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, suppressible | partially by crbj1B5/crb[+]
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, suppressible | partially by mam2/mam[+]
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, suppressible | partially by NAx-16/N[+]
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, suppressible | partially by e(r)G926
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, suppressible | partially by N[+]/N55e11
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, suppressible | partially by r-le01755
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, suppressible by Hsap\EOGTTub.PM
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, non-suppressible by dpyv/e(dpyv)1
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, non-suppressible by pot[+]/pote04564
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, non-suppressible by mys1/mys[+]
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, non-suppressible by pio[+]/pio2R-16
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, non-suppressible by pio[+]/pioMB03570
Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU has wing blade posterior compartment | heat sensitive phenotype, non-suppressible by Mmus\Pomgnt2Tub.PM
dplv1 and dpolvR each dominantly enhance the wing blistering phenotype seen in the posterior compartment of wings in flies expressing EogtGD5084 under the control of Scer\GAL4en.PU in the presence of Dcr-2Scer\UAS.cDa, such that the phenotype is seen at 22.5[o]C.
dpv ; e(dpv)1 does not enhance or suppress the wing blistering phenotype seen in the posterior compartment of wings in flies expressing EogtGD5084 under the control of Scer\GAL4en.PU in the presence of Dcr-2Scer\UAS.cDa.
The wing blistering phenotype seen in the posterior compartment of wings in flies expressing EogtGD5084 under the control of Scer\GAL4en.PU in the presence of Dcr-2Scer\UAS.cDa is not affected if the flies are also heterozygous for either pio2R-16, pioMB03570, pote04564 or mys1.
The wing blistering phenotype seen in the posterior compartment of wings in flies expressing EogtGD5084 under the control of Scer\GAL4en.PU in the presence of Dcr-2Scer\UAS.cDa is enhanced if the flies are also heterozygous for wbSF11.
The wing blistering phenotype seen in the posterior compartment of wings in flies expressing EogtGD5084 under the control of Scer\GAL4en.PU in the presence of Dcr-2Scer\UAS.cDa is weakly but significantly suppressed if the flies are also heterozygous for crbj1B5.
The wing blistering phenotype seen in the posterior compartment of wings in flies expressing EogtGD5084 under the control of Scer\GAL4en.PU in the presence of Dcr-2Scer\UAS.cDa is dominantly partially suppressed if the flies are also heterozygous for one of NAx-16, NAx-E2, NAx-9, Nspl-1, Df(1)N-264-105, N55e11, Df(3R)ED6237, Df(3R)ED5942, Df(3L)ri-79c, Df(2L)TE35BC-4, Df(2R)BSC383, Df(1)N-81k1, DlRevF10, SerRX106, PsnI2, Psn227, Su(H)2 or mam2.
The wing blistering phenotype seen in the posterior compartment of wings in flies expressing EogtGD5084 under the control of Scer\GAL4en.PU in the presence of Dcr-2Scer\UAS.cDa is partially suppressed if the flies also carry N+t40 or Dp(1;2)51b.
The wing blistering phenotype seen in the posterior compartment of wings in flies expressing EogtGD5084 under the control of Scer\GAL4en.PU in the presence of Dcr-2Scer\UAS.cDa is robustly suppressed at 27[o]C if the flies also carry r9. At 31[o]C, partial suppression is seen.
The wing blistering phenotype seen in the posterior compartment of wings in flies expressing EogtGD5084 under the control of Scer\GAL4en.PU in the presence of Dcr-2Scer\UAS.cDa is robustly suppressed at 27[o]C if the flies also carry Dhod8, r-lK2 or b1.
The wing blistering phenotype seen in the posterior compartment of wings in flies expressing EogtGD5084 under the control of Scer\GAL4en.PU in the presence of Dcr-2Scer\UAS.cDa is partially suppressed at 27[o]C if the flies also carry r-lK2 or e(r)G926.
In(1)r70b ; rcSa robustly suppresses the wing blistering phenotype seen in the posterior compartment of wings in flies expressing EogtGD5084 under the control of Scer\GAL4en.PU in the presence of Dcr-2Scer\UAS.cDa at 27[o]C.
In(1)r70b ; rSu(b).cSa partially suppresses the wing blistering phenotype seen in the posterior compartment of wings in flies expressing EogtGD5084 under the control of Scer\GAL4en.PU in the presence of Dcr-2Scer\UAS.cDa at 27[o]C.
b1 ; pyd3Lb5 enhances the wing blistering phenotype seen in the posterior compartment of wings in flies expressing EogtGD5084 under the control of Scer\GAL4en.PU in the presence of Dcr-2Scer\UAS.cDa.
b1 ; pyd3+t3.8 ; pyd3Lb10 partially suppresses the wing blistering phenotype seen in the posterior compartment of wings in flies expressing EogtGD5084 under the control of Scer\GAL4en.PU in the presence of Dcr-2Scer\UAS.cDa at 27[o]C.
Expression of Hsap\EOGTTub.PM completely suppresses the wing blistering phenotype seen in the posterior compartment of wings at 27[o]C in flies expressing EogtGD5084 under the control of Scer\GAL4en.PU in the presence of Dcr-2Scer\UAS.cDa.
Expression of Mmus\Gtdc2Tub.PM fails to suppress the wing blistering phenotype seen in the posterior compartment of wings in flies expressing EogtGD5084 under the control of Scer\GAL4en.PU in the presence of Dcr-2Scer\UAS.cDa.