Allele Dmel\CanA1^GD16976

General Information
Symbol	Dmel\CanA1^GD16976	Species	D. melanogaster
Name		FlyBase ID	FBal0206511
Feature type	allele	Associated gene	Dmel\CanA1

Allele class
Mutagen	in vitro construct - RNAi, in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Dickson et al., 2007.7.18) in vitro construct - RNAi (Dickson et al., 2007.7.18)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an inverted repeat. (Dickson et al., 2007.7.18)
Carried in construct	P{GD16976} (Dickson et al., 2007.7.18, Tomita et al., 2011, Mummery-Widmer et al., 2009)
Associated Sequence Features	dsRNA-GD16976
Cytology
Phenotypic Data
Phenotypic Class
	hypoactive, with Scer\GAL4^elav-C155 (Tomita et al., 2011) memory defective, with Scer\GAL4^elav-C155 (Tomita et al., 2011) partially lethal - majority die \| pupal stage, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) sleep defective, with Scer\GAL4^elav-C155 (Tomita et al., 2011) visible, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009)
Phenotype Manifest In
	mesothoracic tergum, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) trichogen cell, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009)
Detailed Description
	Statement Reference Total daily sleep of flies expressing CanA1[GD16976] in all neurons under the control of Scer\GAL4[elav-C155] decreases to about half or one-fifth that of control flies, respectively. Total sleep for subjective day and subjective night also decreases significantly in these flies. These flies exhibit a substantial circadian rhythmicity of locomotor activity at an approximate 24 hour period in dark/dark conditions. Approximately 16% of flies expressing CanA1[GD16976] in all neurons under the control of Scer\GAL4[elav-C155] are aroused by a weak stimulus compared with 43% of control flies. In response to a strong stimulus, approximately 52% of CanA1[GD16976]-expressing flies are aroused, compared to 86% of controls. Despite a short sleep phenotype, sleeping CanA1[GD16976] flies are hypo-responsive to mechanical stimuli compared to control flies. In contrast, spontaneous arousal during the subjective night is not significantly different between these flies and controls. Flies expressing CanA1[GD16976] in all neurons under the control of Scer\GAL4[elav-C155] are able to compensate for acute sleep loss. After mechanical sleep deprivation for 12 hours during subjective night, these flies exhibit a homeostatic response. The immediate memory (3 min memory) of flies expressing CanA1[GD16976] in all neurons under the control of Scer\GAL4[elav-C155] is indistinguishable from that of control flies. In contrast, the 2 hour memory of these flies decreases significantly compared with control flies, indicating a defect in the maintenance of memory rather than the acquisition of memory. This defective memory retention is not attributable to abnormal sensorimotor responses as these flies respond normally to both odours (3-octanol and 4-methylcyclohexanol) and to electrical shock. (Tomita et al., 2011) Expression under the control of Scer\GAL4[pnr-MD237] results in bristle morphology defects on the notum in 0% or 10-20% of the Scer\GAL4[pnr-MD237] expression domain, depending on the insertion line used. Expression under the control of Scer\GAL4[pnr-MD237] results in the absence of 60-70% or 90-100% of the Scer\GAL4[pnr-MD237]-expressing area of the notum, depending on the insertion line used. (Mummery-Widmer et al., 2009)
External Data
Linkouts	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 16976
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 2 )
VDRC	v49986 w¹¹¹⁸; P{GD16976}v49986/CyO v49987 w¹¹¹⁸; P{GD16976}v49987/CyO
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts
	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 16976
Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	CanA1^GD16976
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Tomita et al., 2011, J. Neurosci. 31(37): 13137--13146 Pan-Neuronal Knockdown of Calcineurin Reduces Sleep in the Fruit Fly, Drosophila melanogaster. [FBrf0215571]
Supplementary material	Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]

Allele Dmel\CanA1GD16976

Allele Dmel\CanA1^GD16976