Flies expressing CanB2^GD13435 in all neurons, under the control of Scer\GAL4^elav-C155, display significant hyperactivity compared to control flies under both light/dark and dark/dark conditions. On average, total daily activity increases significantly by two- to threefold compared to wild-type. These flies show very low-amplitude rhythms because of the high level of baseline activity at all times. these flies also show a small diurnal variation in sleep amount. These flies show a markedly shorter sleep bout length compared with controls.

Flies expressing CanB2^GD13435 in all neurons under the control of Scer\GAL4^elav-C155 are able to compensate for acute sleep loss. After mechanical sleep deprivation for 12 hours during subjective night, these flies exhibit a homeostatic response.

Flies expressing CanB2^GD13435 in all neurons under the control of Scer\GAL4^elav-C155 show an impairment in both olfactory sensing and electrical shock response.

Adults expressing CanB2^GD13435 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in early pupal lethality or late pupal lethality.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 20-30% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.