Primary hemocytes from larvae expressing Chc^GD14095 under the control of Scer\GAL4^srp.PU show strongly reduced internalization of sSpi-GFP protein (spi encodes the principal ligand of the EGFR pathway) at low sSpi-GFP conditions (treatment with 4 ng/ml of sSpi-GFP) but not at high sSpi-GFP conditions (treatment with 30 ng/ml of sSpi-GFP), as compared to controls.

Third larval instar adipocytes expressing Chc^GD14095 under the control of Scer\GAL4^{SPARC-MI00329-GAL4} show a significant increase in both plasma membrane depth and sinuosity compared to control cells.

Expression of Chc^GD14095 under the control of Scer\GAL4^AB1 results in a complete block in glue granule formation in most larval salivary gland cells, with only rare cells exhibiting small granules. Pupal cases are poorly adherent.

Expression of Chc^GD14095 under the control of Scer\GAL4^Appl.G1a disrupts synaptic vesicle endocytosis. Synaptic vesicle size is increased in these flies with respect to wild-type. Boutons exhibit large cisternae-like membrane structure rarely seen in wild-type. The number of docked synaptic vesicles at Chc^GD14095 synapses is increased with respect to wild-type, indicating that synaptic vesicles are present at release sites but unable to fuse efficiently with the plasma membrane.

Synapses expressing Chc^GD14095 under the control of Scer\GAL4^Appl.G1a display an activity dependent reduction in excitatory post-synaptic current amplitude.

Flies expressing Chc^GD14095 under the control of Scer\GAL4^Appl.G1a produce an activity-dependent reduction in excitatory post-synaptic current (EPSC) amplitude with rapid onset during train stimulation. Vesicle size is increased in these mutants.

Adults expressing Chc^GD14095 under the control of the cardioblast-specific Scer\GAL4^tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

Adults expressing Chc^GD14095 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression under the control of Scer\GAL4^Mef2.PR results in early pupal lethality.

Expression under the control of Scer\GAL4^pnr-MD237 results in lethality before the pupal stage.