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General Information
Symbol
Dmel\ChcGD14095
Species
D. melanogaster
Name
FlyBase ID
FBal0206558
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Primary hemocytes from larvae expressing ChcGD14095 under the control of Scer\GAL4srp.PU show strongly reduced internalization of sSpi-GFP protein (spi encodes the principal ligand of the EGFR pathway) at low sSpi-GFP conditions (treatment with 4 ng/ml of sSpi-GFP) but not at high sSpi-GFP conditions (treatment with 30 ng/ml of sSpi-GFP), as compared to controls.

Third larval instar adipocytes expressing ChcGD14095 under the control of Scer\GAL4SPARC-MI00329-GAL4 show a significant increase in both plasma membrane depth and sinuosity compared to control cells.

Expression of ChcGD14095 under the control of Scer\GAL4AB1 results in a complete block in glue granule formation in most larval salivary gland cells, with only rare cells exhibiting small granules. Pupal cases are poorly adherent.

Expression of ChcGD14095 under the control of Scer\GAL4Appl.G1a disrupts synaptic vesicle endocytosis. Synaptic vesicle size is increased in these flies with respect to wild-type. Boutons exhibit large cisternae-like membrane structure rarely seen in wild-type. The number of docked synaptic vesicles at ChcGD14095 synapses is increased with respect to wild-type, indicating that synaptic vesicles are present at release sites but unable to fuse efficiently with the plasma membrane.

Synapses expressing ChcGD14095 under the control of Scer\GAL4Appl.G1a display an activity dependent reduction in excitatory post-synaptic current amplitude.

Flies expressing ChcGD14095 under the control of Scer\GAL4Appl.G1a produce an activity-dependent reduction in excitatory post-synaptic current (EPSC) amplitude with rapid onset during train stimulation. Vesicle size is increased in these mutants.

Adults expressing ChcGD14095 under the control of the cardioblast-specific Scer\GAL4tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

Adults expressing ChcGD14095 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression under the control of Scer\GAL4Mef2.PR results in early pupal lethality.

Expression under the control of Scer\GAL4pnr-MD237 results in lethality before the pupal stage.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
ChcGD14095
Name Synonyms
Secondary FlyBase IDs
    References (10)