Eggs laid by flies expressing Cp36GD6311 under the control of Scer\GAL4c355 have a thin and fragile chorion, a highly dysmorphic dorsal appendages as well as malformed operculum, collar and aeropyle. The eggshell surface also lacks the typical follicular imprints. On the ultrastructural level, Cp36GD6311 knock-down leads to severely disrupted organization of the endochorion, while the exochorion, the inner chorionic layer and the vitelline membrane are not significantly affected. These eggs seem incapable of being fertilized by sperm as they fail to hatch and no post-zygotic mitotic nuclei are found in them.
Expression of Cp36GD6311 driven by Scer\GAL4c522 or Scer\GAL4109C1 or Scer\GAL4109-28 does not affect the flies' fecundity as both the pupariation and eclosion rate is comparable to controls. And while with the first two drivers no structural defects in the laid eggs are observe, aged females (30-60 days old) females expressing Cp36GD6311 under the Scer\GAL4109-28 driver occasionally lay eggs with either extra (rather normal-looking) appendages or severely deformed and fused appendages, this is not seen in either age-matched controls or in younger knock-down flies.
Adults expressing Cp36GD6311 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.