FlyBase Allele Report: Dmel\Dcr-1[GD11429]

General Information

Symbol

Dmel\Dcr-1^GD11429

Species

D. melanogaster

Name

FlyBase ID

FBal0206827

Feature type

allele

Associated gene

Dmel\Dcr-1

Associated Insertion(s)

Carried in Construct

P{GD11429}

Also Known As

UAS-dcr-1^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD11429}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD11429

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

wing blade, with Dcr-1^Q1147X, Scer\GAL4^en-e16E

(Herranz et al., 2010)

wing blade, with Scer\GAL4^en-e16E

(Herranz et al., 2010)

wing blade, with Scer\GAL4^ptc-559.1

(Herranz et al., 2010)

wing disc | third instar larval stage, with Scer\GAL4^ptc-559.1

(Herranz et al., 2010)

Detailed Description

Statement

Reference

Expression of Dcr-1^GD11429 in the fat body under the control of Scer\GAL4^ppl.PP increases the survival rates of both males and female adult flies subjected to nutrient starvation when compared to control flies.

(Barrio et al., 2014)

Expression of Scer\GAL4^en-e16E>Dcr-1^GD11429 engenders an autonomous reduction in cell size the adult wing. As a consequence, the size of the adult wing is also reduced.

Co-expression of Dcr-1^Scer\UAS.cDa restores cell and tissue size in wings expressing Scer\GAL4^en-e16E>Dcr-1^GD11429.

Dcr-1^Q1147X heterozygosity enhances the wing size defects caused by the expression of Scer\GAL4^en-e16E>Dcr-1^GD11429. The viability of these flies is drastically reduced.

Wing disc cells expressing Scer\GAL4^ptc-559.1>Dcr-1^GD11429 show G[[1]]-S cell cycle delay.

(Herranz et al., 2010)

Adults expressing Dcr-1^GD11429 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of Dcr-1^GD11429 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or complete lethality, depending on the particular P{GD11429} insertion line used.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

11429

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Dcr-1^GD11429, Scer\GAL4^ppl.PP has abnormal starvation stress response phenotype, suppressible by p53^H159N.UAS.Ex, Scer\GAL4^ppl.PP

(Barrio et al., 2014)

Dcr-1^GD11429, Scer\GAL4^en-e16E has decreased cell growth rate phenotype, suppressible by dap[+]/dap⁴

(Herranz et al., 2010)

Dcr-1^GD11429, Scer\GAL4^en-e16E has abnormal size phenotype, suppressible by Myc^UAS.cZa, Scer\GAL4^en-e16E

(Herranz et al., 2010)

Dcr-1^GD11429, Scer\GAL4^en-e16E has decreased cell growth rate phenotype, suppressible by Myc^UAS.cZa, Scer\GAL4^en-e16E

(Herranz et al., 2010)

Dcr-1^GD11429, Scer\GAL4^en-e16E has abnormal size phenotype, suppressible by Myc^hs.PSA

(Herranz et al., 2010)

Dcr-1^GD11429, Scer\GAL4^en-e16E has decreased cell growth rate phenotype, suppressible by Myc^hs.PSA

(Herranz et al., 2010)

Dcr-1^GD11429, Scer\GAL4^en-e16E has abnormal size phenotype, suppressible by Myc^αTub84B.PBb

(Herranz et al., 2010)

Dcr-1^GD11429, Scer\GAL4^en-e16E has decreased cell growth rate phenotype, suppressible by Myc^αTub84B.PBb

(Herranz et al., 2010)

Dcr-1^GD11429, Scer\GAL4^en-e16E has decreased cell growth rate phenotype, suppressible by mei-P26^KK106754, Scer\GAL4^en-e16E

(Herranz et al., 2010)

Dcr-1^GD11429, Scer\GAL4^en-e16E has abnormal size phenotype, suppressible by mir-ban^hs.PH

(Herranz et al., 2010)

Dcr-1^GD11429, Scer\GAL4^en-e16E has decreased cell growth rate phenotype, suppressible by mir-ban^hs.PH

(Herranz et al., 2010)

Dcr-1^GD11429, Scer\GAL4^en-e16E has abnormal size phenotype, suppressible by CycE^UAS.cLa, Scer\GAL4^en-e16E

(Herranz et al., 2010)

Dcr-1^GD11429, Scer\GAL4^en-e16E has decreased cell growth rate phenotype, suppressible by CycE^UAS.cLa, Scer\GAL4^en-e16E

(Herranz et al., 2010)

Dcr-1^GD11429, Scer\GAL4^en-e16E has abnormal size phenotype, suppressible by dap[+]/dap⁴

(Herranz et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

Dcr-1^GD11429, Scer\GAL4^en-e16E has wing blade phenotype, suppressible by CycE^UAS.cLa, Scer\GAL4^en-e16E

(Herranz et al., 2010)

Dcr-1^GD11429, Scer\GAL4^en-e16E has wing blade phenotype, suppressible by dap[+]/dap⁴

(Herranz et al., 2010)

Dcr-1^GD11429, Scer\GAL4^en-e16E has wing blade phenotype, suppressible by Myc^UAS.cZa, Scer\GAL4^en-e16E

(Herranz et al., 2010)

Dcr-1^GD11429, Scer\GAL4^en-e16E has wing blade phenotype, suppressible by Myc^hs.PSA

(Herranz et al., 2010)

Dcr-1^GD11429, Scer\GAL4^en-e16E has wing blade phenotype, suppressible by Myc^αTub84B.PBb

(Herranz et al., 2010)

Dcr-1^GD11429, Scer\GAL4^en-e16E has wing blade phenotype, suppressible | partially by mei-P26^KK106754, Scer\GAL4^en-e16E

(Herranz et al., 2010)

Dcr-1^GD11429, Scer\GAL4^en-e16E has wing blade phenotype, suppressible by mir-ban^hs.PH

(Herranz et al., 2010)

Suppressor of

Statement

Reference

Dcr-1^GD11429/Scer\GAL4^vg.int2.1 is a suppressor of dorsal-ventral compartment boundary of the wing disc | third instar larval stage phenotype of N^RNAi.P.UAS

(Becam et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of p53^{H159N.Scer\UAS.Ex} suppresses the increase in survival rate in response to starvation in adult flies expressing Dcr-1^GD11429 in the fat body under the control of Scer\GAL4^ppl.PP.

(Barrio et al., 2014)

Expression of Dcr-1^GD11429 largely rescues the dorsal-ventral boundary defects seen when N^{dsRNA.P.Scer\UAS} is expressed under the control of Scer\GAL4^vg.int2.1.

(Becam et al., 2011)

Co-expression of CycE^Scer\UAS.cLa with Scer\GAL4^en-e16E>Dcr-1^GD11429 restores cell and wing size.

Heterozygous dap⁴ restores cell and wing size in animals expressing Scer\GAL4^en-e16E>Dcr-1^GD11429.

The tissue and cell size defects caused by Scer\GAL4^en-e16E>Dcr-1^GD11429 in adult wings are largely suppressed when dm^Scer\UAS.cZa is co-expressed.

The tissue and cell size defects caused by Scer\GAL4^en-e16E>Dcr-1^GD11429 in adult wings are largely suppressed when dm^hs.PSA is co-expressed.

The tissue and cell size defects caused by Scer\GAL4^en-e16E>Dcr-1^GD11429 in adult wings are largely suppressed when dm^αTub84B.PBb is co-expressed.

The cell size defects caused by Scer\GAL4^en-e16E>Dcr-1^GD11429 in adult wings are largely suppressed when mei-P26^KK106754 is co-expressed.

ban^hs.PH expression can rescue the tissue and size defects caused by Scer\GAL4^en-e16E>Dcr-1^GD11429 in adult wings.

(Herranz et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v24666

w¹¹¹⁸; P{GD11429}v24666

v24667

w¹¹¹⁸; P{GD11429}v24667

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

11429

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Dcr-1^GD11429

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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