FlyBase Allele Report: Dmel\Dis3[GD11917]

General Information

Symbol

Dmel\Dis3^GD11917

Species

D. melanogaster

Name

FlyBase ID

FBal0206863

Feature type

allele

Associated gene

Dmel\Dis3

Associated Insertion(s)

Carried in Construct

P{GD11917}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD11917}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD11917

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | larval stage, with Scer\GAL4^nub-AC-62

(Towler et al., 2015)

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

increased cell death | third instar larval stage, with Scer\GAL4^nub-AC-62

(Towler et al., 2015)

lethal, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

lethal | pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

lethal - all die before end of second instar larval stage, with Scer\GAL4^69B

(Towler et al., 2015)

lethal - all die before end of second instar larval stage, with Scer\GAL4^da.G32

(Towler et al., 2015)

lethal - all die before end of second instar larval stage, with Scer\GAL4^αTub84B.PL

(Towler et al., 2015)

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

partially lethal - majority die | pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^nub-AC-62

(Towler et al., 2015)

visible, with Scer\GAL4^pnr-MD237

(Towler et al., 2015, Mummery-Widmer et al., 2009)

Phenotype Manifest In

adult thorax, with Scer\GAL4^pnr-MD237

(Towler et al., 2015)

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

haltere, with Scer\GAL4^nub-AC-62

(Towler et al., 2015)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing blade, with Scer\GAL4^nub-AC-62

(Towler et al., 2015)

wing disc | third instar larval stage, with Scer\GAL4^nub-AC-62

(Towler et al., 2015)

wing pouch | third instar larval stage, with Scer\GAL4^nub-AC-62

(Towler et al., 2015)

Detailed Description

Statement

Reference

Expression of Dis3^GD11917 driven by Scer\GAL4^nub-AC-62 results in a complete absence of wing blade development (no wing phenotype) and abnormal haltere in adults with 100% penetrance, and late L3 imaginal discs are significantly smaller than controls and show extensive apoptosis in the wing pouch region. Scer\GAL4^nub-AC-62>Dis3^GD11917 flies show delayed larval development (by 40 hours at 25[o]C). Expression of Dis3^GD11917 driven by Scer\GAL4^pnr-MD237 results in a cleft thorax phenotype in adults with 100% penetrance.

(Towler et al., 2015)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

(Neumüller et al., 2011)

Adults expressing Dis3^GD11917 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in early pupal lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in lethality or semi-lethality at the pupal stage, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 100% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

11917

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v35090

w¹¹¹⁸; P{GD11917}v35090

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 2 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

11917

Neuroblasts Screen Database (Knoblich Lab) - A database for RNAi phenotypes in larval neural stem cells

11917

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Dis3^GD11917

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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