Allele Dmel\Egfr^GD1654

General Information
Symbol	Dmel\Egfr^GD1654	Species	D. melanogaster
Name		FlyBase ID	FBal0206949
Feature type	allele	Associated gene	Dmel\Egfr

Map ( GBrowse )
Allele class
Mutagen	in vitro construct - RNAi, in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Dickson et al., 2007.7.18) in vitro construct - RNAi (Dickson et al., 2007.7.18)
Mutations Mapped to the Genome

Type Location Additional Notes References RNAi reagent :1..284
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an inverted repeat. (Dickson et al., 2007.7.18)
Carried in construct	P{GD1654} (Dickson et al., 2007.7.18, Zettl et al., 2011, Yue et al., 2012, Corl et al., 2009, Jeon and Zinn, 2009, Ren et al., 2010, Mummery-Widmer et al., 2009, Lee et al., 2011)
Associated Sequence Features	dsRNA-GD1654
Cytology
Phenotypic Data
Phenotypic Class
	body color defective, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) chemical sensitive, with Scer\GAL4^elav-C155 (Corl et al., 2009) viable, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) visible, with Scer\GAL4^GMR.PF (Corl et al., 2009)
Phenotype Manifest In
	eye, with Scer\GAL4^GMR.PF (Corl et al., 2009) mesothoracic tergum, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009)
Detailed Description
	Statement Reference Males expressing Egfr[GD1654] under the control of Scer\GAL4[Abd-B-LDN] show normal genitalia rotation. (Lee et al., 2011) Expression of Egfr[GD1654] under the control of Scer\GAL4[elav-C155] results in flies with increased sensitivity to ethanol-induced sedation compared to control flies. Expression of Egfr[GD1654] under the control of Scer\GAL4[GMR.PF] results in a mild rough eye phenotype. (Corl et al., 2009) Expression under the control of Scer\GAL4[pnr-MD237] results in a colour difference between the central Scer\GAL4[pnr-MD237] expression domain of the notum and the surrounding lateral region in 30-40% or 80-90% of the Scer\GAL4[pnr-MD237] expression domain, depending on the insertion line used. Expression under the control of Scer\GAL4[pnr-MD237] results in the absence of 10-20% or 20-30% of the Scer\GAL4[pnr-MD237]-expressing area of the notum, depending on the insertion line used. (Mummery-Widmer et al., 2009)
External Data
Linkouts	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 1654
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Egfr^GD1654, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has visible phenotype, enhanceable by hppy^RB.Scer\UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Corl et al., 2009)
Suppressed by
Statement Reference Egfr^GD1654, Scer\GAL4^elav-C155 has chemical sensitive phenotype, suppressible by hppy^17-51 (Corl et al., 2009) Egfr^GD1654, Scer\GAL4^elav-C155 has chemical sensitive phenotype, suppressible by hppy^KG05537 (Corl et al., 2009)
Suppressor of
Statement Reference Scer\GAL4^elav-C155/Egfr^GD1654 is a suppressor \| partially of sleep defective \| recessive phenotype of rho-5^KO1 (Zettl et al., 2011) Scer\GAL4^esg-NP5130/Egfr^GD1654 is a suppressor of increased cell number \| somatic clone \| cell autonomous phenotype of hpo^BF33 (Ren et al., 2010) Scer\GAL4^esg-NP5130/Egfr^GD1654 is a suppressor of mitotic cell cycle defective \| somatic clone \| cell autonomous phenotype of hpo^BF33 (Ren et al., 2010)
NOT Suppressor of
Statement Reference Scer\GAL4^esg-NP5130/Egfr^GD1654 is a non-suppressor of increased cell number \| somatic clone \| cell non-autonomous phenotype of hpo^BF33 (Ren et al., 2010) Scer\GAL4^esg-NP5130/Egfr^GD1654 is a non-suppressor of mitotic cell cycle defective \| somatic clone \| cell non-autonomous phenotype of hpo^BF33 (Ren et al., 2010)
Phenotype Manifest In
Enhanced by
Statement Reference Egfr^GD1654, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, enhanceable by hppy^RB.Scer\UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Corl et al., 2009)
Suppressor of
Statement Reference Scer\GAL4^btl.PS/Egfr^GD1654 is a suppressor of embryonic/larval tracheal system phenotype of Ptp10D¹, Ptp4E¹ (Jeon and Zinn, 2009) Scer\GAL4^esg-NP5130/Egfr^GD1654 is a suppressor of intestinal stem cell \| somatic clone phenotype of hpo^BF33 (Ren et al., 2010)
NOT Suppressor of
Statement Reference Egfr^GD1654/Scer\GAL4^Abd-B-LDN is a non-suppressor of male genitalia phenotype of Uvrag^KG04163/Uvrag^B21 (Lee et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference Expression of Egfr[GD1654] under the control of Scer\GAL4[elav-C155] significantly suppresses the sleep defects seen in homozygous rho-5[KO1] mutants. (Zettl et al., 2011) Compared with hpo[BF33] mutant MARCM clones, hpo[BF33] mutant clones expressing Egfr[GD1654] exhibit reduced clone size. Egfr[GD1654] expression suppresses the elevated levels of mitosis seen in hpo[BF33] MARCM clones but not outside the expression domain of the MARCM clones. (Ren et al., 2010) The mild rough eye phenotype caused by expression of Egfr[GD1654] under the control of Scer\GAL4[GMR.PF] is potently enhanced by co-expression of hppy[RB.Scer\UAS]. The increased sensitivity to ethanol-induced sedation caused by expression of Egfr[GD1654] under the control of Scer\GAL4[elav-C155] is completely suppressed by either hppy[KG05537] or hppy[17-51]. (Corl et al., 2009)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 2 )
VDRC	v43267 w¹¹¹⁸; P{GD1654}v43267 v43268 w¹¹¹⁸; P{GD1654}v43268/CyO
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts
	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 1654
Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Egfr^GD1654
Name Synonym
Secondary FlyBase IDs

References ( 8 )
Research paper	Yue et al., 2012, Dev. Cell 22(2): 255--267 The cell adhesion molecule echinoid functions as a tumor suppressor and upstream regulator of the hippo signaling pathway. [FBrf0217474] Lee et al., 2011, Dev. Biol. 356(2): 588--597 UVRAG is required for organ rotation by regulating Notch endocytosis in Drosophila. [FBrf0214470] Zettl et al., 2011, Cell 145(1): 79--91 Rhomboid Family Pseudoproteases Use the ER Quality Control Machinery to Regulate Intercellular Signaling. [FBrf0213387] Ren et al., 2010, Proc. Natl. Acad. Sci. U.S.A. 107(49): 21064--21069 Hippo signaling regulates Drosophila intestine stem cell proliferation through multiple pathways. [FBrf0212502] Corl et al., 2009, Cell 137(5): 949--960 Happyhour, a Ste20 family kinase, implicates EGFR signaling in ethanol-induced behaviors. [FBrf0208186] Jeon and Zinn, 2009, Development 136(18): 3121--3129 Receptor tyrosine phosphatases control tracheal tube geometries through negative regulation of Egfr signaling. [FBrf0208606]
Supplementary material	Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]

Allele Dmel\EgfrGD1654

Allele Dmel\Egfr^GD1654