FlyBase Allele Report: Dmel\Fatp1[GD1069]

General Information

Symbol

Dmel\Fatp1^GD1069

Species

D. melanogaster

Name

FlyBase ID

FBal0207012

Feature type

allele

Associated gene

Dmel\Fatp1

Associated Insertion(s)

Carried in Construct

P{GD1069}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1069}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1069

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal feeding behavior, with Scer\GAL4^Lsp2.PH

(Sujkowski et al., 2012)

decreased fecundity | progressive, with Scer\GAL4^Desat1.PB

(Poidevin et al., 2024)

female sterile, with Scer\GAL4^Desat1.PB

(Poidevin et al., 2024)

female sterile, with Scer\GAL4^insc-Mz1407

(Poidevin et al., 2024)

lethal - all die during larval stage, with Scer\GAL4^sal.BO

(Wicker-Thomas et al., 2015)

long lived, with Scer\GAL4^Lsp2.PH

(Sujkowski et al., 2012)

long lived, with Scer\GAL4^Mef2.PR

(Sujkowski et al., 2012)

long lived, with Scer\GAL4^Tub.PU

(Sujkowski et al., 2012)

male fertile, with Scer\GAL4^insc-Mz1407

(Poidevin et al., 2024)

partially lethal - majority die, with Scer\GAL4^da.G32

(Sujkowski et al., 2012)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

adult heart, with Scer\GAL4^UAS.cHa, Scer\GAL4^tin.cBa

(Sujkowski et al., 2012)

adult oenocyte, with Scer\GAL4^insc-Mz1407

(Wicker-Thomas et al., 2015)

embryonic/larval tracheal section | larval stage, with Scer\GAL4^sal.BO

(Wicker-Thomas et al., 2015)

lipid droplet | adult stage, with Scer\GAL4^insc-Mz1407

(Wicker-Thomas et al., 2015)

Detailed Description

Statement

Reference

Expression of Fatp^GD1069 under the control of Scer\GAL4^sal.BO (in combination with a Dicer-2 transgene to enhance RNAi efficiency) results in lethality at the L2/L3 transition, accompanied by tracheal defects.

Adult oenocytes of flies expressing Fatp^GD1069 under the control of Scer\GAL4^insc-Mz1407 contain more lipid droplets as compared to controls.

(Wicker-Thomas et al., 2015)

Adults expressing Fatp^GD1069 under the control of Scer\GAL4^Lsp2.PH have significantly increased triglyceride levels compared to age-matched control flies.

Adults expressing Fatp^GD1069 under the control of Scer\GAL4^Lsp2.PH show a significantly reduced feeding rate compared to controls.

Expression of Fatp^GD1069 under the control of either Scer\GAL4^tub.PU or Scer\GAL4^Lsp2.PH results in an increase in lifespan compared to controls in both males and females.

Expression of Fatp^GD1069 under the control of Scer\GAL4^Mef2.PR results in an increase in lifespan compared to controls. This lifespan extension is unaffected by exercise treatment.

The rate of cardiac failure in response to pacing stress is significantly higher in adults expressing Fatp^GD1069 under the simultaneous control of both Scer\GAL4^tin.cBa and Scer\GAL4^Scer\UAS.cHa compared to controls. The frequency of the heart rate is normal in these flies, but the mutant hearts show a reduction in fractional shortening.

(Sujkowski et al., 2012)

Adults expressing Fatp^GD1069 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1069

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

Fatp1^GD1069, Scer\GAL4^insc-Mz1407 is a non-suppressor of adult oenocyte phenotype of Acc^GD3482, Scer\GAL4^insc-Mz1407

(Wicker-Thomas et al., 2015)

Fatp1^GD1069, Scer\GAL4^insc-Mz1407 is a non-suppressor of lipid droplet | adult stage phenotype of Acc^GD3482, Scer\GAL4^insc-Mz1407

(Wicker-Thomas et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Fatp^GD1069 fails to suppress the increase in lipid droplets seen in oenocytes of flies expressing ACC^GD3482 under the control of Scer\GAL4^insc-Mz1407.

(Wicker-Thomas et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v9406

w¹¹¹⁸; P{GD1069}v9406

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1069

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Fatp1^GD1069

Fatp^GD1069

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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