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General Information
Symbol
Dmel\Fer1HCHGD4853
Species
D. melanogaster
Name
FlyBase ID
FBal0207025
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
fer1HCH RNAi, UAS-Fer1HCH-RNAi
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expressing Fer1HCHGD4853 under the control of Scer\GAL4NP3084 is lethal in flies fed an iron-depleted diet and only partially lethal (majority die) when flies are fed a regular or iron-rich diet. Expression under the control of either Scer\GAL4elav-C155 or Scer\GAL4GMR.PFa results in slightly smaller eyes with a rough appearance.

Expression of Fer1HCHGD4853 under the control of Scer\GAL4Lsp2.3.1 (using tub-Gal80[ts] to limit expression to adulthood) does not induce cell death in either germaria or vitellogenic stage follicles and while it does not affect the proliferation of the germline stem cells it leads to significant increase in the rate of their loss over time.

Scer\GAL4NP3084-mediated expression of Fer1HCHGD4853 decreases total body iron content and causes death at larval/pupal stages.

Expression with P{GAL4-tim.E}27, in the presence of Dcr-2Scer\UAS.cDa, results in lethality.

Adults expressing Fer1HCHGD4853 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression under the control of Scer\GAL4Mef2.PR results in early larval lethality.

Expression under the control of Scer\GAL4Mef2.PR results in grossly normal larval body wall muscles.

Expression under the control of Scer\GAL4Mef2.PR results in wild-type sarcomeres in the larval body wall muscles.

Expression under the control of Scer\GAL4pnr-MD237 results in lethality before the pupal stage.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Statement
Reference

Fer1HCHGD4853, Scer\GAL4NP3084 has lethal | nutrition conditional phenotype, non-enhanceable by Tsf1UAS.cXa, Scer\GAL4NP3084

Suppressed by
NOT suppressed by
Enhancer of
Other
Phenotype Manifest In
NOT suppressed by
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Co-expressing Tsf1KK103815 with Fer1HCHGD4853 under the control of Scer\GAL4NP3084 results in partial lethality (majority die) regardless of iron content in the diet.

The low eclosion rate and decreased total body iron content of Scer\GAL4NP3084 Fer1HCHGD4853 flies can be partially rescued by co-expression of Zip99CScer\UAS.cXa.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Fer1HCHGD4853
Name Synonyms
Secondary FlyBase IDs
    References (15)