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General Information
Symbol
Dmel\Hrb27CGD6964
Species
D. melanogaster
Name
FlyBase ID
FBal0207319
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expressing Hrb27CGD6964 under the control of Scer\GAL4ey.PH results in a reduced overall eye size, while expression under the control of Scer\GAL4mirr-DE leads to an almost complete ablation of the eye dorsal compartment in adults. Expression under the control of Scer\GAL4hh-Gal4 causes a severe reduction of the wing disc posterior compartment in third instar larvae.

Somatic clones expressing Hrb27CGD6964 under the control of Scer\GAL4αTub84B.PL in eye discs of third instar larvae display reduced cell proliferation (determined by EdU incorporation).

Expressing Hrb27CGD6964 under the control of Scer\GAL4ptc-559.1 results in increased cell death compared to controls in wing discs of third instar larvae, while expression under the control of Scer\GAL4hh-Gal4 causes a reduction in cell density in the posterior compartment. Expression under the control of Scer\GAL4ptc-559.1 has no effect on the size of the anterior-posterior compartment boundary of the wing disc.

Expression of Hrb27CGD6964 under the control of Scer\GAL4GMR.PU has no obvious effect on adult eye morphology, whereas expression driven by Scer\GAL4elav.PU is lethal.

Adults expressing Hrb27CGD6964 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of Hrb27CGD6964 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or partial lethality, depending on the particular P{GD6964} insertion line used.

Expression of Hrb27CGD6964 in the developing eye under the control of Scer\GAL4GMR.PF does not affect eye development.

Expression under the control of Scer\GAL4Mef2.PR results in late pupal lethality.

Expression under the control of Scer\GAL4pnr-MD237 results in extra bristles on the notum in 0% or 10-20% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4pnr-MD237 results in loss of bristles on the notum in 10-20% or 20-30% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4pnr-MD237 results in bristle morphology defects on the notum in 30-40% or 40-50% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4pnr-MD237 results in the absence of 40-50% or 50-60% of the Scer\GAL4pnr-MD237-expressing area of the notum, depending on the insertion line used.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Animals expressing Hrb27CGD6964 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4elav.PU in either wild-type or TBPHΔ23/+; TBPHdsRNA.Scer\UAS.cUa background are lethal.

The eye degeneration phenotype (appearance of melanotic spots, fusion of ommatidia) characteristic for adult flies expressing TBPHScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.PU is strongly suppressed by co-expression of Hrb27CGD6964.

Expression of Hrb27CGD6964 in the developing eye dominantly suppresses the eye degeneration phenotype found upon expression of TER94R152H.Scer\UAS.R (with both transgenes driven by Scer\GAL4GMR.PF). This is concurrent with a significant reduction in the blinded phenotypic severity score.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Hrb27CGD6964
Name Synonyms
Secondary FlyBase IDs
    References (10)