FlyBase Allele Report: Dmel\Hrb98DE[GD14939]

General Information

Symbol

Dmel\Hrb98DE^GD14939

Species

D. melanogaster

Name

FlyBase ID

FBal0207321

Feature type

allele

Associated gene

Dmel\Hrb98DE

Associated Insertion(s)

Carried in Construct

P{GD14939}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD14939}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD14939

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

DOES NOT ameliorate spinocerebellar ataxia type 31

modeled by Zzzz\UGGAA^W.UAS

(Ishiguro et al., 2017)

modeled by Zzzz\UGGAA^W.UAS, Hsap\TARDBP^UAS.cIb

(Ishiguro et al., 2017)

modeled by Zzzz\UGGAA^S.UAS

(Ishiguro et al., 2017)

modeled by Zzzz\UGGAA^S.UAS, Hsap\TARDBP^UAS.cIb

(Ishiguro et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body color, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

abnormal planar polarity, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

flightless, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

lethal, with Scer\GAL4^Act5C.PI

(Ji and Tulin, 2012)

lethal, with Scer\GAL4^αTub84B.PL

(Ji and Tulin, 2012)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

chaeta | increased number, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

myofibril, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

sarcomere, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Z disc, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

Detailed Description

Statement

Reference

Ubiquitous expression of Hrb98DE^GD14939 under the control of Scer\GAL4^Act5C.PI causes lethality.

Ubiquitous expression of Hrb98DE^GD14939 under the control of Scer\GAL4^αTub84B.PL causes lethality.

(Ji and Tulin, 2012)

Adults expressing Hrb98DE^GD14939 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal adult indirect flight muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in frayed myofibrils in the adult indirect flight muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in sarcomeres with broadened Z-lines in the adult indirect flight muscles.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in extra bristles on the notum in 40-50% or 50-60% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in a colour difference between the central Scer\GAL4^pnr-MD237 expression domain of the notum and the surrounding lateral region in 0% or 30-40% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 40-50% or 50-60% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in planar polarity defects in the orientation of bristles of the notum in 40-50% or 50-60% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 40-50% or 50-60% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

14939

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

Hrb98DE^GD14939, Scer\GAL4^GMR.PS is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.PS, Zzzz\UGGAA^W.UAS

(Ishiguro et al., 2017)

Hrb98DE^GD14939, Scer\GAL4^GMR.PS is a non-suppressor of abnormal size | adult stage phenotype of Hsap\TARDBP^UAS.cIb, Scer\GAL4^GMR.PS, Zzzz\UGGAA^S.UAS

(Ishiguro et al., 2017)

Hrb98DE^GD14939, Scer\GAL4^GMR.PS is a non-suppressor of abnormal size | adult stage phenotype of Scer\GAL4^GMR.PS, Zzzz\UGGAA^W.UAS

(Ishiguro et al., 2017)

Hrb98DE^GD14939, Scer\GAL4^GMR.PS is a non-suppressor of abnormal eye color phenotype of Scer\GAL4^GMR.PS, Zzzz\UGGAA^W.UAS

(Ishiguro et al., 2017)

Hrb98DE^GD14939, Scer\GAL4^GMR.PS is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.PS, Zzzz\UGGAA^S.UAS

(Ishiguro et al., 2017)

Hrb98DE^GD14939, Scer\GAL4^GMR.PS is a non-suppressor of abnormal size | adult stage phenotype of Scer\GAL4^GMR.PS, Zzzz\UGGAA^S.UAS

(Ishiguro et al., 2017)

Hrb98DE^GD14939, Scer\GAL4^GMR.PS is a non-suppressor of abnormal eye color phenotype of Scer\GAL4^GMR.PS, Zzzz\UGGAA^S.UAS

(Ishiguro et al., 2017)

Hrb98DE^GD14939, Scer\GAL4^GMR.PS is a non-suppressor of visible | adult stage phenotype of Hsap\TARDBP^UAS.cIb, Scer\GAL4^GMR.PS, Zzzz\UGGAA^S.UAS

(Ishiguro et al., 2017)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

Hrb98DE^GD14939, Scer\GAL4^GMR.PS is a non-suppressor of eye | adult stage phenotype of Scer\GAL4^GMR.PS, Zzzz\UGGAA^W.UAS

(Ishiguro et al., 2017)

Hrb98DE^GD14939, Scer\GAL4^GMR.PS is a non-suppressor of eye | adult stage phenotype of Scer\GAL4^GMR.PS, Zzzz\UGGAA^S.UAS

(Ishiguro et al., 2017)

Hrb98DE^GD14939, Scer\GAL4^GMR.PS is a non-suppressor of eye | adult stage phenotype of Hsap\TARDBP^UAS.cIb, Scer\GAL4^GMR.PS, Zzzz\UGGAA^S.UAS

(Ishiguro et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The co-expression of Hrb98DE^GD14939 does not lead to a decrease in the severity of the eye defects resulting from the Scer\GAL4^GMR.PS-driven expression of either Zzzz\UGGAA^W.Scer\UAS or Zzzz\UGGAA^S.Scer\UAS, alone or in combination with Hsap\TARDBP^Scer\UAS.cIb (i.e. reduced size, loss of pigmentation, defective morphology and ommatidial abnormalities).

(Ishiguro et al., 2017)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v29523

w¹¹¹⁸; P{GD14939}v29523

v29524

w¹¹¹⁸; P{GD14939}v29524/CyO