Allele Dmel\JhI-1^GD10927

General Information
Symbol	Dmel\JhI-1^GD10927	Species	D. melanogaster
Name		FlyBase ID	FBal0207405
Feature type	allele	Associated gene	Dmel\JhI-1

Map ( GBrowse )
Allele class
Mutagen	in vitro construct - RNAi, in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Dickson et al., 2007.7.18) in vitro construct - RNAi (Dickson et al., 2007.7.18)
Mutations Mapped to the Genome

Type Location Additional Notes References RNAi reagent :1..400
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an inverted repeat. (Dickson et al., 2007.7.18)
Carried in construct	P{GD10927} (Dickson et al., 2007.7.18, Neumüller et al., 2011, Mummery-Widmer et al., 2009, Xie et al., 2011)
Associated Sequence Features	dsRNA-GD10927
Cytology
Phenotypic Data
Phenotypic Class
	decreased cell growth, with Scer\GAL4^da.G32 (Xie et al., 2011) decreased cell growth, with Scer\GAL4^hs.PB (Xie et al., 2011) lethal (Neumüller et al., 2011) lethal \| second instar larval stage, with Scer\GAL4^da.G32 (Xie et al., 2011) lethal \| third instar larval stage, with Scer\GAL4^da.G32 (Xie et al., 2011) lethal \| third instar larval stage, with Scer\GAL4^αTub84B.PL (Xie et al., 2011) neuroanatomy defective \| larval stage (Neumüller et al., 2011) partially lethal - majority die \| female limited \| pharate adult stage, with Scer\GAL4^A9 (Xie et al., 2011) partially lethal - majority die \| female limited \| pupal stage, with Scer\GAL4^A9 (Xie et al., 2011) partially lethal - majority die \| pharate adult stage (Xie et al., 2011) viable, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) visible, with Scer\GAL4^ey.PH (Xie et al., 2011) visible, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) visible \| female limited, with Scer\GAL4^A9 (Xie et al., 2011)
Phenotype Manifest In
	chaeta, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) dorsal wing blade \| female limited, with Scer\GAL4^A9 (Xie et al., 2011) eye, with Scer\GAL4^ey.PH (Xie et al., 2011) eye-antennal disc, with Scer\GAL4^ey.PH (Xie et al., 2011) ganglion mother cell \| larval stage (Neumüller et al., 2011) head capsule, with Scer\GAL4^ey.PH (Xie et al., 2011) mesothoracic tergum, with Scer\GAL4^ap-md544 (Xie et al., 2011) mesothoracic tergum, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) ommatidium, with Scer\GAL4^ey.PH (Xie et al., 2011) scutellum, with Scer\GAL4^ap-md544 (Xie et al., 2011) trichogen cell, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) wing blade, with Scer\GAL4^ap-md544 (Xie et al., 2011)
Detailed Description
	Statement Reference Expression under the control of Scer\GAL4[1407] results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls. Expression under the control of Scer\GAL4[1407] may result in mild defects in the shape of ganglion mother cells in the larval brain (depending on the insertion line used). (Neumüller et al., 2011) Scer\GAL4[da.G32]>JhI-1[GD10927] embryos are completely viable. They do not show any patterning defects and they hatch into larvae at the same time and size as wild-type control animals. Initially, the knockdown larvae show a healthy development and by the end of the second day AED (after egg deposition) they are able to molt successfully into the second instar. However, from the very beginning, knockdown larvae grow more slowly than the control and after the first molt almost stop gaining weight. They survive to another 3-4 days only reaching about a 15-fold increase in mass over newly hatched larvae. The control animals develop normally reaching about 200-fold increase in mass by the end of larval development and pupariate 5-6 days AED. At approximately the same time, a small portion of the Scer\GAL4[da.G32]>JhI-1[GD10927] larvae die as second instars (as demonstrated by the morphology of the mouth hooks), but most knockdown animals manage to initiate the transition to third instar and die during molting either with both second and third instar mouth hooks attached or with a partially shed cuticle. Practically the same phenotype is observed for with another ubiquitous driver, Scer\GAL4[αTub84B.PL]. Scer\GAL4[hs.PB]>JhI-1[GD10927] animals are completely viable, though upon pupariation, knockdown larvae are unable to adhere themselves tightly to the wall of the vial. Salivary glands dissected from knockdown white prepupae are significantly smaller than those from wild-type control animals of the same age, and rather have the size of the glands from the second instar. There is no significant difference in the total number of cells in salivary glands from knockdown larvae as compared to controls. However, Scer\GAL4[hs.PB]>JhI-1[GD10927] causes a decrease in cell size, nuclear size and DNA content. Although knockdown nuclei fail to attain normal size, they are noticeably bigger and packed tighter than control nuclei of the second instar. At around 72 hours AED, the nuclei of Scer\GAL4[hs.PB]>JhI-1[GD10927] knockdown salivary glands do not show any growth, but do undergo an additional round of endoreplication reaching ploidy of 64C. During second instar (72 hours AED), endoreplication is practically normal in Scer\GAL4[hs.PB]>JhI-1[GD10927] animals. During third instar, knockdown cells show a significant reduction in DNA synthesis, although several endocycling nuclei are still detected. Males and females expressing JhI-1[GD10927] under the control of Scer\GAL4[A9] appear normal. The number of knockdown females is significantly smaller than expected. About 67% of them die as late pupae or pharate adults. The wing blades of female escapers are bent upward suggesting that the dorsal surface of the wing is reduced. All the animals expressing JhI-1[GD10927] under the control of Scer\GAL4[ap-md544] dies during metamorphosis. Dissected Scer\GAL4[ap-md544]>JhI-1[GD10927] pharate adults reveal a whole range of dramatic phenotypes in the thoracic segment: tiny wing blades, absent scutellum, and split notum. About 76% of animals expressing JhI-1[GD10927] under the control of Scer\GAL4[ey.PH] die as pharate adults. The surviving 24% display a range of phenotypes in one or both eyes, such as small eye, split eye, or no eye. Knockdown animals with small eyes display a significant reduction of the ommatidial number and a severe disruption of the regular ommatidial array, compared with wild-type. The mean size of the ommatidia is not significantly reduced in knockdowns, indicating that cell size is not affected by expression of JhI-1[GD10927] in mitotic cells. When animals exhibiting pupal lethality are dissected from their puparia, the pharate adults reveal a headless phenotype, missing all or most of the structures derived from eye-antennal imaginal discs, resulting in reduced head capsules. Expression of JhI-1[GD10927] in the predominantly postmitotic cells posterior to the morphogenetic furrow of the developing eye under the control of Scer\GAL4[GMR.PF] does not reduce viability. The eyes of flies expressing JhI-1[GD10927] under the control of Scer\GAL4[GMR.PF] do not show any eye abnormalities. Even 30 days post eclosion, eye size and ommatidial integrity appear similar in knockdown and control flies. (Xie et al., 2011) Expression under the control of Scer\GAL4[pnr-MD237] results in loss of bristles on the notum in 40-50% or 90-100% of the Scer\GAL4[pnr-MD237] expression domain, depending on the insertion line used. Expression under the control of Scer\GAL4[pnr-MD237] results in bristle morphology defects on the notum in 10-20% or 60-70% of the Scer\GAL4[pnr-MD237] expression domain, depending on the insertion line used. Expression under the control of Scer\GAL4[pnr-MD237] results in the absence of 50-60% of the Scer\GAL4[pnr-MD237]-expressing area of the notum. (Mummery-Widmer et al., 2009)
External Data
Linkouts	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 10927 Neuroblasts Screen Database - A database for RNAi phenotypes in larval neural stem cells • 10927
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	JhI-1^GD10927 is rescued by JhI-1^{Scer\UAS.P\T.T:SV5\V5}/Scer\GAL4^da.G32 (Xie et al., 2011)
Comments	Scer\GAL4[da.G32]>JhI-1[GD10927] knockdown animals can be rescued to adulthood by co-expression of JhI-1[Scer\UAS.P\T.T:SV5\V5]. (Xie et al., 2011)
Stocks ( 2 )
VDRC	v43751 w¹¹¹⁸; P{GD10927}v43751 v43752 w¹¹¹⁸; P{GD10927}v43752
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts
	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 10927 Neuroblasts Screen Database - A database for RNAi phenotypes in larval neural stem cells • 10927
Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	JhI-1^GD10927
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Xie et al., 2011, Insect Biochem. Mol. Biol. 41(3): 167--177 RNAi knockdown of dRNaseZ, the Drosophila homolog of ELAC2, impairs growth of mitotic and endoreplicating tissues. [FBrf0213094]
Supplementary material	Neumüller et al., 2011, Cell Stem Cell 8(5): Supplemental Table S1. [FBrf0214431] Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]

Allele Dmel\JhI-1GD10927

Allele Dmel\JhI-1^GD10927