Third instar larvae expressing Lis-1GD1480 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4ppk.1.9 display hyposensitive behavioral response (lengthened latency till response to 46[o]C thermal probe stimulus) in a thermal nociception assay. Lis-1GD1480 RNAi expression also leads to significantly decreased dendrite coverage in the ddaC dorsal multidendritic neurons compared to controls.
Larvae expressing Lis-1GD1480 under the control of Scer\GAL4Hml.PG show normal rates of encapsulation of eggs when parasitised by the avirulent L. boulardi wasp strain G486.
Delays in border cell migration are seen in females in which all follicle cells express Lis-1GD1480 under the control of Scer\GAL4Act.PU.
A large fraction of border follicle cells have not initiated migration at stages 9 and 10 in females co-expressing Lis-1GD1480 and Lis-1KK108813 under the control of Scer\GAL4Act.PU in follicle cell clones. The mutant clusters are more rounded than controls at the stage when migration should initiate. The early extensions from the front cell which are normally seen before and at the start of active cluster movement are not rarer in the mutant clusters and do not reach the same size as in controls. The small fraction of mutant border cell clusters that do initiate migration show a greatly reduced net migration speed compared to wild type and the size and frequency of forward cell extensions is severely reduced.
Expression under the control of Scer\GAL41407 results in an increased number of GFP-positive aggregates within neuroblast lineage cells in the larval brain compared to controls (these aggregates may indicate cell death or a disturbance of internal cellular structures).
Expression of Lis-1GD1480 under the control of Scer\GAL4upd results in a reduction in the number of outer border follicle cells compared to wild type and also results in defects in border follicle cell migration. Expression under the control of Scer\GAL4slbo.2.6 does not cause these phenotypes.
Adults expressing Lis-1GD1480 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies.