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General Information
Symbol
Dmel\Lis-1GD1480
Species
D. melanogaster
Name
FlyBase ID
FBal0207511
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Third instar larvae expressing Lis-1GD1480 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4ppk.1.9 display hyposensitive behavioral response (lengthened latency till response to 46[o]C thermal probe stimulus) in a thermal nociception assay. Lis-1GD1480 RNAi expression also leads to significantly decreased dendrite coverage in the ddaC dorsal multidendritic neurons compared to controls.

Larvae expressing Lis-1GD1480 under the control of Scer\GAL4Hml.PG show normal rates of encapsulation of eggs when parasitised by the avirulent L. boulardi wasp strain G486.

Delays in border cell migration are seen in females in which all follicle cells express Lis-1GD1480 under the control of Scer\GAL4Act.PU.

A large fraction of border follicle cells have not initiated migration at stages 9 and 10 in females co-expressing Lis-1GD1480 and Lis-1KK108813 under the control of Scer\GAL4Act.PU in follicle cell clones. The mutant clusters are more rounded than controls at the stage when migration should initiate. The early extensions from the front cell which are normally seen before and at the start of active cluster movement are not rarer in the mutant clusters and do not reach the same size as in controls. The small fraction of mutant border cell clusters that do initiate migration show a greatly reduced net migration speed compared to wild type and the size and frequency of forward cell extensions is severely reduced.

Expression under the control of Scer\GAL41407 results in an increased number of GFP-positive aggregates within neuroblast lineage cells in the larval brain compared to controls (these aggregates may indicate cell death or a disturbance of internal cellular structures).

Expression of Lis-1GD1480 under the control of Scer\GAL4upd results in a reduction in the number of outer border follicle cells compared to wild type and also results in defects in border follicle cell migration. Expression under the control of Scer\GAL4slbo.2.6 does not cause these phenotypes.

Adults expressing Lis-1GD1480 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 2 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Neuroblasts Screen Database (Knoblich Lab) - A database for RNAi phenotypes in larval neural stem cells
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Lis-1GD1480
lis-1GD1480
Name Synonyms
Secondary FlyBase IDs
    References (13)