FB2025_01 , released February 20, 2025
Allele: Dmel\MarfGD11094
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General Information
Symbol
Dmel\MarfGD11094
Species
D. melanogaster
Name
FlyBase ID
FBal0207590
Feature type
allele
Associated gene
Dmel\Marf
Associated Insertion(s)
Carried in Construct
P{GD11094}
Also Known As
marf RNAi, UAS-MarfRNAi
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
in vitro construct
(Dickson et al., 2007.7.18)
Progenitor genotype
Carried in construct
P{GD11094}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-GD11094
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  Charcot-Marie-Tooth disease
      CEA
      (Debattisti et al., 2014)
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of MarfGD11094 under the control of Scer\GAL4RapGAP1-OK6 results in increased mitochondrial circularity (due to decreased fusion) at the distal ends of both anterior (synapsing at segment 3) and posterior (synapsing at segment 7) motor neuron axons in third instar larvae. Expression under the Scer\GAL4nSyb.PS driver leads to a rapid age-dependent decline in adult climbing abilities and decreased adult lifespan.

      (Fowler and O'Sullivan, 2016)

      Expression of MarfGD11094 under the control of Scer\GAL4Mef2.PR results in small fragmented mitochondria in adult indirect flight muscles, deficit in larval locomotion (reduced crawling speed) and partial lethality as the adult eclosion rate is decreased by about 50% relative to controls.

      (Wang et al., 2016)

      Neuronal expression of MarfGD11094 (under the control of Scer\GAL4elav.PU) causes significant lipid droplet accumulation in glia but not neurons.

      Glial expression of MarfGD11094 (under the control of Scer\GAL454C) does not lead to lipid droplet accumulation.

      (Liu et al., 2015)

      In Scer\GAL4tub.PU MarfGD11094 larvae, mitochondria are clustered and fragmented in ventral ganglion neuronal cell bodies, and small and clumped in ventral ganglion axons. Likewise, mitochondria appear clustered in the perinuclear region in smaller muscles. The ER has punctiform and individual cisternae, having lost its sarcomeric organization.

      ~10% of Scer\GAL4Mef2.PR MarfGD11094 reach adulthood.

      In Scer\GAL4elav.PLu MarfGD11094 larvae, mitochondria are clustered and fragmented in neuronal cell bodies and fragmented ER cisternae accumulate in the cell cortex.

      In Scer\GAL4Mef2.PR MarfGD11094 larvae, mitochondria are clumped around the nuclei and the SR has punctiform and individual cisternae, having lost their sarcomeric organization.

      Scer\GAL4Mhc.PU MarfGD11094 adults show impaired climbing ability.

      Scer\GAL4Act.PU- or Scer\GAL4tub.PU-mediated expression of MarfGD11094 results in upregulation of reporters of ER stress.

      (Debattisti et al., 2014)

      Expression of MarfGD11094 in the posterior signalling center (PSC) under the control of either Scer\GAL4Antp-10 leads to an increase in the number of circulating lamellocytes.

      (Sinenko et al., 2011)

      Mitochondria in the indirect flight muscles are shorter in the long-axis direction than normal in 5 day old adults expressing MarfGD11094 under the control of Scer\GAL4Mhc.PW.

      (Imai et al., 2010)

      Adults expressing MarfGD11094 under the control of the cardioblast-specific Scer\GAL4tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

      (Neely et al., 2010)

      Adults expressing MarfGD11094 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

      (Neely et al., 2010)

      Expression under the control of Scer\GAL4Mef2.PR results in late pupal lethality.

      (Schnorrer et al., 2010)

      Expression under the control of Scer\GAL4pnr-MD237 results in bristle morphology defects on the notum in 100% of the Scer\GAL4pnr-MD237 expression domain.

      (Mummery-Widmer et al., 2009)

      Expression of MarfGD11094 under the control of Scer\GAL4GMR.PF does not result in any detectable eye defects.

      (Poole et al., 2008)
      External Data
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      NOT suppressed by
      Enhancer of
      Suppressor of
      Phenotype Manifest In
      Enhanced by
      NOT Enhanced by
      Suppressed by
      NOT suppressed by
      Enhancer of
      Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The increased midgut cell size along with their enlarged mitochondria observed in pupae (2hr after puparium formation) expressing Vps13DGD10464 under the control of Scer\GAL4Myo31DF-NP0001 is rescued by co-expression of MarfGD11094.

      (Anding et al., 2018)

      The rapid age-dependent loss of climbing abilities and reduced lifespan characteristic for adult flies expressing MarfGD11094 under the control of Scer\GAL4nSyb.PS is exacerbated by co-expression of Arl6IP1GD3027. However, the increase in mitochondrial circularity induced by Scer\GAL4RapGAP1-OK6-driven expression of MarfGD11094 in the both anterior (synapsing to segment 3) and posterior (synapsing to segment 7) motor neuron axons in third instar larvae is not greatly affected by co-expression with Arl6IP1GD3027, which on its own induces mitochondrial elongation in the posterior axons.

      (Fowler and O'Sullivan, 2016)

      The mitochondrial fragmentation observed in indirect flight muscles of adult flies expressing MarfGD11094 under the Scer\GAL4Mef2.PR driver appears to be further enhanced by co-expression of cluUAS.Tag:MYC.

      The larval locomotor deficit, very low adult eclosion rate as well as the mitochondrial morphology defects in adult indirect flight muscles (enlarged clustered mitochondria) characteristic for animals expressing cluGD13926 under the control of Scer\GAL4Mef2.PR can be suppressed to a varying degree by co-expression of MarfGD11094.

      (Wang et al., 2016)

      Pgam51 does not suppress the abnormal mitochondrial phenotype (mitochondria in the indirect flight muscles are shorter in the long-axis direction than normal) seen in 5 day old adults expressing MarfGD11094 under the control of Scer\GAL4Mhc.PW.

      (Imai et al., 2010)

      The defect in mitochondrial morphology in the indirect flight muscles which is seen in porinA2 homozygotes is suppressed by expression of MarfGD11094 under the control of Scer\GAL4Mef2.PR.

      (Park et al., 2010)

      Expression of MarfGD11094 under the control of Scer\GAL4hs.PB strongly suppresses the downturned wing and crushed thorax phenotypes of Pink1B9 mutant flies and the flightless phenotype is partially suppressed.

      (Park et al., 2009)

      The rough eye phenotype of flies expressing Pink1Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF is enhanced by co-expression of MarfGD11094.

      (Poole et al., 2008)
      Xenogenetic Interactions
      Statement
      Reference

      Expression of Hsap\MFN2Scer\UAS.T:Hsap\MYC in a Scer\GAL4elav.PLu MarfGD11094 background allows survival to adulthood of ~50% of individuals. Neuronal cell body mitochondria remain fragmented but ER morphology is restored.

      Expression of Hsap\MFN2Scer\UAS.T:Hsap\MYC in a Scer\GAL4Mef2.PR MarfGD11094 background allows greater survival to adulthood, and SR, but not mitochondrial, morphology is restored.

      Co-expression of Hsap\MFN2Scer\UAS.T:Hsap\MYC, but not Hsap\MFN1Scer\UAS.T:Hsap\MYC or Hsap\MFN2R94Q.Scer\UAS.T:Hsap\MYC rescues the impaired climbing ability of Scer\GAL4Mhc.PU MarfGD11094 flies.

      Co-expression of Mmus\Xbp1Scer\UAS.cCTa suppresses the impaired climbing ability of Scer\GAL4Mhc.PU MarfGD11094 flies.

      (Debattisti et al., 2014)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      MarfGD11094
      Name Synonyms
      Secondary FlyBase IDs
        References (21)