Allele Dmel\Mef2^GD5039

General Information
Symbol	Dmel\Mef2^GD5039	Species	D. melanogaster
Name		FlyBase ID	FBal0207605
Feature type	allele	Associated gene	Dmel\Mef2

Map ( GBrowse )
Allele class
Mutagen	in vitro construct - RNAi, in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Dickson et al., 2007.7.18) in vitro construct - RNAi (Dickson et al., 2007.7.18)
Mutations Mapped to the Genome

Type Location Additional Notes References RNAi reagent :1..300
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an inverted repeat. (Dickson et al., 2007.7.18)
Carried in construct	P{GD5039} (Dickson et al., 2007.7.18, Dietzl et al., 2007, Bryantsev et al., 2012, Bernard et al., 2009, Blanchard et al., 2010, Mummery-Widmer et al., 2009, van Eyk et al., 2011, Chen et al., 2012)
Associated Sequence Features	dsRNA-GD5039
Cytology
Phenotypic Data
Phenotypic Class
	flight defective, with Scer\GAL4^elav.PLu (Dietzl et al., 2007) lethal, with Scer\GAL4^Mef2.PR (Dietzl et al., 2007) lethal \| pharate adult stage, with Scer\GAL4¹¹⁵¹ (Bryantsev et al., 2012) locomotor rhythm defective, with Scer\GAL4^P0.5.Pdf (Blanchard et al., 2010) locomotor rhythm defective, with Scer\GAL4^tim.Scer\UAS (Blanchard et al., 2010) partially lethal - majority die, with Scer\GAL4^elav.PLu (Dietzl et al., 2007) viable, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) visible, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009)
Phenotype Manifest In
	adult somatic muscle, with Scer\GAL4¹¹⁵¹ (Bryantsev et al., 2012) dorsal medial muscle \| pupal stage, with Scer\GAL4¹¹⁵¹ (Bryantsev et al., 2012) dorsal medial muscle \| pupal stage, with Scer\GAL4^Act88F.PB (Bryantsev et al., 2012) embryonic/larval somatic muscle, with Scer\GAL4^Mef2.PR (Chen et al., 2012) indirect flight muscle \| pupal stage, with Mef2^{dsRNA.IR2.Scer\UAS}, Scer\GAL4^Act88F.PB (Bryantsev et al., 2012) indirect flight muscle \| pupal stage, with Mef2^P544, Scer\GAL4^Act88F.PB (Bryantsev et al., 2012) indirect flight muscle \| pupal stage, with Scer\GAL4¹¹⁵¹ (Bryantsev et al., 2012) indirect flight muscle \| pupal stage, with Scer\GAL4^Act88F.PB (Bryantsev et al., 2012) mesothoracic extracoxal depressor muscle 66, with Scer\GAL4^Act79B.PB (Bryantsev et al., 2012) mesothoracic tergum, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) trichogen cell, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) wing, with Scer\GAL4^elav.PLu (Dietzl et al., 2007)
Detailed Description
	Statement Reference Animals expressing Mef2[GD5039] under the control of Scer\GAL4[1151] develop normally but remain in their pupal cases and eventually die, unable to eclose. The animals lack essentially all major somatic muscles in their bodies. Cardiac and visceral muscles are unaffected. In developing pupae expressing Mef2[GD5039] under the control of Scer\GAL4[1151] the development of the indirect flight muscles is severely affected due to a lack of myoblast fusion to form myofibre syncytia. The larval oblique muscles which form the templates for the dorsal longitudinal muscles do not undergo splitting at 24 hours after puparium formation (APF), in contrast to wild type. These templates are smaller than normal in the mutant pupae, whereas the layer of swarming myoblasts surrounding them is significantly thicker than normal. Groups of myoblasts are seen at the putative sites of dorso-ventral muscle formation in the mutant pupae, but they are never accompanied by a fusion core. Fused myofibres are not seen in the thorax of mutant animals at 30 hours APF, but instead large numbers of unfused myoblasts are apparent. At 48 hours APF there is still a lack of fused myotubes, but the number of unfused myoblasts is significantly decreased (they are detected only as small groups of loosely aggregated cells). The dorsal longitudinal muscles often collapse to the back of the thorax by 48 hours after puparium formation in animals expressing Mef2[GD5039] under the control of Scer\GAL4[Act88F.PB]. The dorso-ventral muscles (DVMs) develop normal looking myofibrils by the end of the pupal stage, although they are somewhat wavy in their organisation. The morphology of the DVM myofibrils is worsened if the animals also carry either Mef2[P544] or Mef2[dsRNA.IR2.Scer\UAS]. Expression of Mef2[GD5039] under the control of Scer\GAL4[Act79B.PB] results in moderate but persistent changes in morphology in the tergal depressor of the trochanter muscle, including the presence of a central cavity within the myofibre rosette, mis-localised and aggregated nuclei and a reduction in overall muscle size. (Bryantsev et al., 2012) Embryos expressing Mef2[GD5039] under the control of Scer\GAL4[Mef2.PR] show muscle extension and attachment defects. Larvae show a significant reduction in the number of body wall contractions per minute compared to controls. (Chen et al., 2012) Expression of Mef2[GD5039] under the control of Scer\GAL4[P0.5.Pdf] (in the presence of Dcr-2[Scer\UAS.cDa] to increase RNAi efficiency) causes long locomotor activity rhythms, with a period of 25.0 +/- 0.4 hours. These rhythms have a much lower power than in control flies (32.6 compared to 80.7). Expression of Mef2[GD5039] under the control of Scer\GAL4[tim.Scer\UAS] (in the presence of Dcr-2[Scer\UAS.cDa] to increase RNAi efficiency) results in 70% of the flies becoming progressively arrhythmic over a 12 day period. Adult LN[[v]] neurons are present and project normally when Mef2[GD5039] is expressed under the control of Scer\GAL4[tim.Scer\UAS]. (Blanchard et al., 2010) Expression under the control of Scer\GAL4[pnr-MD237] results in bristle morphology defects on the notum in 0% or 20-30% of the Scer\GAL4[pnr-MD237] expression domain, depending on the insertion line used. Expression under the control of Scer\GAL4[pnr-MD237] results in the absence of 60-70% or 80-90% of the Scer\GAL4[pnr-MD237]-expressing area of the notum, depending on the insertion line used. (Mummery-Widmer et al., 2009) Expression of Mef2^GD5039 under the control of Scer\GAL4^elav.PLu leads to a variable phenotype depending on the insertion, with flies showing variable levels of lethality and some flies showing abnormal wing posture. Scer\GAL4^elav.PLu>Mef2^GD5039 flies also show flight defects. (Dietzl et al., 2007)
External Data
Linkouts	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 5039
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference Mef2^GD5039, Scer\GAL4^GMR.PFa is an enhancer of visible phenotype of Scer\GAL4^GMR.PFa, Zzzz\CTG^{86.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} (van Eyk et al., 2011)
NOT Enhancer of
Statement Reference Mef2^GD5039, Scer\GAL4^GMR.PFa is a non-enhancer of visible phenotype of Scer\GAL4^GMR.PFa, Zzzz\CAG^{93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} (van Eyk et al., 2011) Mef2^GD5039, Scer\GAL4^GMR.PFa is a non-enhancer of visible phenotype of Scer\GAL4^GMR.PFa, Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} (van Eyk et al., 2011)
NOT Suppressor of
Statement Reference Mef2^GD5039, Scer\GAL4^GMR.PFa is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PFa, Zzzz\CAG^{93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} (van Eyk et al., 2011) Mef2^GD5039, Scer\GAL4^GMR.PFa is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PFa, Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} (van Eyk et al., 2011)
Other
Statement Reference Mef2^GD5039, Scer\GAL4^GMR.PFa, Zzzz\CTG^{term.CTG.99.Scer\UAS} has visible phenotype (van Eyk et al., 2011)
Phenotype Manifest In
Enhancer of
Statement Reference Mef2^GD5039, Scer\GAL4^GMR.PFa is an enhancer of eye phenotype of Scer\GAL4^GMR.PFa, Zzzz\CTG^{86.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} (van Eyk et al., 2011)
NOT Enhancer of
Statement Reference Mef2^GD5039, Scer\GAL4^GMR.PFa is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PFa, Zzzz\CAG^{93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} (van Eyk et al., 2011) Mef2^GD5039, Scer\GAL4^GMR.PFa is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PFa, Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} (van Eyk et al., 2011)
NOT Suppressor of
Statement Reference Mef2^GD5039, Scer\GAL4^GMR.PFa is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PFa, Zzzz\CAG^{93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} (van Eyk et al., 2011) Mef2^GD5039, Scer\GAL4^GMR.PFa is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PFa, Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} (van Eyk et al., 2011)
Other
Statement Reference Mef2^GD5039, Scer\GAL4^GMR.PFa, Zzzz\CTG^{term.CTG.99.Scer\UAS} has eye phenotype (van Eyk et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Co-expression of Mef2[GD5039] with Zzzz\CAG[93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] under the control of Scer\GAL4[GMR.PFa] does not alter the appearance of the eye. Co-expression of Mef2[GD5039] with Zzzz\CAG[CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] under the control of Scer\GAL4[GMR.PFa] does not alter the appearance of the eye. Co-expression of Mef2[GD5039] with Zzzz\CTG[86.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] under the control of Scer\GAL4[GMR.PFa] causes an enhancement of the Zzzz\CTG[86.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG]-induced eye phenotype, resulting in flies with smaller eyes which have a glazed appearance and necrotic patches. Co-expression of Mef2[GD5039] with Zzzz\CTG[term.CTG.99.Scer\UAS] under the control of Scer\GAL4[GMR.PFa] results in a marked disruption of the pigmentation and patterning of the eye. Co-expression of Mef2[GD5039] with Zzzz\CAG[term93.Scer\UAS] under the control of Scer\GAL4[GMR.PFa] does not disrupt eye patterning. Co-expression of Mef2[GD5039] with Zzzz\CAG[term.CAA.114.Scer\UAS] under the control of Scer\GAL4[GMR.PFa] does not disrupt eye patterning. Co-expression of Mef2[GD5039] with Hsap\ATXN10[rAUUCU.65.Scer\UAS] under the control of Scer\GAL4[GMR.PFa] does not disrupt eye patterning. (van Eyk et al., 2011)
Complementation & Rescue Data
Comments
Stocks ( 2 )
VDRC	v15550 w¹¹¹⁸; P{GD5039}v15550 v15549 w¹¹¹⁸; P{GD5039}v15549/TM3
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts
	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 5039
Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Mef2^GD5039
Name Synonym
Secondary FlyBase IDs

References ( 8 )
Research paper	Bryantsev et al., 2012, Dev. Biol. 361(2): 191--207 Differential requirements for Myocyte Enhancer Factor-2 during adult myogenesis in Drosophila. [FBrf0217023] Chen et al., 2012, Development 139(19): 3543--3552 miR-92b regulates Mef2 levels through a negative-feedback circuit during Drosophila muscle development. [FBrf0219365] van Eyk et al., 2011, Hum. Mol. Genet. 20(14): 2783--2794 Perturbation of the Akt/Gsk3-{beta} signalling pathway is common to Drosophila expressing expanded untranslated CAG, CUG and AUUCU repeat RNAs. [FBrf0213942] Blanchard et al., 2010, J. Neurosci. 30(17): 5855--5865 The transcription factor Mef2 is required for normal circadian behavior in Drosophila. [FBrf0210652] Bernard et al., 2009, Dev. Biol. 332(2): 258--272 Integration of differentiation signals during indirect flight muscle formation by a novel enhancer of Drosophila vestigial gene. [FBrf0208388] Dietzl et al., 2007, Nature 448(7150): 151--156 A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. [FBrf0200691]
Supplementary material	Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]

Allele Dmel\Mef2GD5039

Allele Dmel\Mef2^GD5039