FlyBase Allele Report: Dmel\Myd88[GD9716]

General Information

Symbol

Dmel\Myd88^GD9716

Species

D. melanogaster

Name

FlyBase ID

FBal0207681

Feature type

allele

Associated gene

Dmel\Myd88

Associated Insertion(s)

Carried in Construct

P{GD9716}

Also Known As

MyD88^IR, UAS-Myd88 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD9716}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD9716

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates carcinoma

modeled by dlg1^A40.2

(Parvy et al., 2019)

ameliorates acute myeloid leukemia

modeled by Mmus\Tet2^{R1261C.UAS.Tag:FLAG}

(Ismail et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response, with Scer\GAL4^c564

(Valanne et al., 2010)

abnormal immune response | adult stage, with Scer\GAL4^c564

(Valanne et al., 2019)

short lived | conditional, with Scer\GAL4^c564

(Valanne et al., 2019)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of Myd88^GD9716 under the control of Scer\GAL4^c564 leads to a significant decrease in survival to a combined M. luteus and E. faecalis bacterial infection, as compared to controls.

(Valanne et al., 2019)

Expression of Myd88^GD9716 RNAi under the control Scer\GAL4^Hml.Δ driver does not rescue the lowered survival of adult flies on lard-supplemented diet compared to controls.

(Woodcock et al., 2015)

Adults expressing Myd88^GD9716 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Flies expressing Myd88^GD9716 under the control of Scer\GAL4^c564 show reduced survival compared to control flies when they are infected with M. luteus and then 24 hours later they are infected with E. faecalis.

(Valanne et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

9716

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^apolpp.PB/Myd88^GD9716 is an enhancer of neoplasia | larval stage phenotype of dlg1^A40.2

(Parvy et al., 2019)

Suppressor of

Statement

Reference

Myd88^GD9716, Scer\GAL4^{Tet-MI05009-GAL4} is a suppressor | partially of increased cell number | larval stage phenotype of Mmus\Tet2^{R1261C.UAS.Tag:FLAG}, Scer\GAL4^{Tet-MI05009-GAL4}

(Ismail et al., 2023)

Scer\GAL4^apolpp.PB/Myd88^GD9716 is a suppressor of increased cell death | larval stage phenotype of dlg1^A40.2

(Parvy et al., 2019)

Scer\GAL4^apolpp.PB, Def^UAS.cTa, Myd88^GD9716 is a suppressor | partially of increased cell death | larval stage phenotype of dlg1^A40.2

(Parvy et al., 2019)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^apolpp.PB/Myd88^GD9716 is an enhancer of wing disc phenotype of dlg1^A40.2

(Parvy et al., 2019)

Suppressor of

Statement

Reference

Myd88^GD9716, Scer\GAL4^{Tet-MI05009-GAL4} is a suppressor | partially of embryonic/larval hemocyte | increased number | larval stage phenotype of Mmus\Tet2^{R1261C.UAS.Tag:FLAG}, Scer\GAL4^{Tet-MI05009-GAL4}

(Ismail et al., 2023)

Scer\GAL4^apolpp.PB/Myd88^GD9716 is a suppressor of wing disc phenotype of dlg1^A40.2

(Parvy et al., 2019)

Scer\GAL4^apolpp.PB, Def^UAS.cTa, Myd88^GD9716 is a suppressor of wing disc phenotype of dlg1^A40.2

(Parvy et al., 2019)

Myd88^GD9716, Scer\GAL4^Hml.Δ is a suppressor of larval sessile hemocyte phenotype of Scer\GAL4^Hml.Δ, Tl^10b.UAS

(Valanne et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The expression of Myd88^GD9716 under the control of Scer\GAL4^apolpp.PB enhances the tumor phenotype of dlg1^A40.2 wing discs, while suppressing the associated apoptosis (Dcp1 staining).

Scer\GAL4^apolpp.PB-driven expression of Def^UAS.cTa in Myd88^GD9716, dlg1^A40.2 double mutants partially re-establishes the original dlg1^A40.2 phenotypes.

(Parvy et al., 2019)

Co-expression of Myd88^GD9716 suppresses the loss of the sessile hemocyte banding pattern which is seen in larvae expressing Tl^10b.Scer\UAS under the control of Scer\GAL4^Hml.Δ.

(Valanne et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v25399