Open Close
General Information
Symbol
Dmel\NGD144
Species
D. melanogaster
Name
FlyBase ID
FBal0207691
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Notch-IR, UAS-Notch RNAi, UAS-Notch-RNAi, N-IR
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The clonal expression of NGD144 under the control of Scer\GAL4Act.PU leads to several polar/stalk cell defects compared to controls: decreased number of polar/stalk cells; stalk cells do not show the normal intercalated morphology; and high frequency of fused egg chambers.

Expression of NGD144 under the control of Scer\GAL4ple.PU as well as the Scer\GAL4elav.PU driver in combination with Scer\GAL80ple.PK (all together with tub-Gal80[ts] to limit the time of expression to adulthood) results in significantly decreased adult lifespan.

Expression of NGD144 under the control of Scer\GAL4insc-Mz1407 results in the loss of type II neuroblasts.

Expression of NGD144 under the control of Scer\GAL4esg-NP7397 in the adult intestine (using the temperature sensitive Scer\GAL80ts.αTub84B allele to limit expression) at 22[o]C results in a mild increase in the density of both enteroendocrine cells and enterocytes, although the ratio of these two differentiated cell types is normal. At 25 and 28[o]C, there is a dramatic increase in the number of intestinal stem cell-like cells, as well as an increase in enteroendocrine cells and concomitant loss of enterocytes.

NGD144 driven by Scer\GAL4sev.PU leads to significant cone cell loss in third instar larvae eye discs, but no defects are observed in the photoreceptors.

Adult flies expressing NGD144 driven by Scer\GAL4sev.PU have glossy retinas and the eyes contain black patches of tissues indicating necrosis.

Adults expressing NGD144 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression under the control of Scer\GAL4Mef2.PR results in late pupal lethality.

Expression under the control of Scer\GAL4pnr-MD237 results in extra bristles on the notum in 10-20% of the Scer\GAL4pnr-MD237 expression domain.

Expression under the control of Scer\GAL4pnr-MD237 results in loss of bristles on the notum in 90-100% of the Scer\GAL4pnr-MD237 expression domain.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT Enhancer of
Suppressor of
NOT Suppressor of
Phenotype Manifest In
Suppressed by
Suppressor of
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

The co-expression of NGD144 (together with Dicer-2 transgene, for efficient RNAi) fully suppresses the increased number of type II neuroblasts in third instar larval brains resulting from the co-expression of lilliScer\UAS.SR.T:Zzzz\FLAG and Su(Tpl)Scer\UAS.SR.T:Hsap\MYC under the control of Scer\GAL4insc-Mz1407, even to the point of near absence of type II neuroblasts, as compared to controls; a similar suppression is not achieved when the phenotype arises from the expression of E(spl)mγ-HLHScer\UAS.cLb alone.

Co-expression of either MarfmiRNA.cUa.Scer\UAS or dmScer\UAS.cZa partially rescues the loss of type II neuroblasts seen in animals expressing NGD144 under the control of Scer\GAL4insc-Mz1407, while simultaneous co-expression of both MarfmiRNA.cUa.Scer\UAS and dmScer\UAS.cZa together fully rescues the phenotype.

wgl-17/+ suppresses the necrosis seen in adult eyes when NGD144 is driven by Scer\GAL4sev.PU.

wgl-17/+ suppresses the cone cell loss phenotype seen in the third instar eye discs when NGD144 is driven by Scer\GAL4sev.PU.

pygoS123/+ suppresses the necrosis seen in adult eyes when NGD144 is driven by Scer\GAL4sev.PU.

pygoS123/+ suppresses the cone cell loss seen in the third instar eye discs when NGD144 is driven by Scer\GAL4sev.PU.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
NGD144
Name Synonyms
Secondary FlyBase IDs
    References (21)