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General Information
Symbol
Dmel\NGD14477
Species
D. melanogaster
Name
FlyBase ID
FBal0207692
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Notch-RNAi, Notch-IR, UAS-Notch RNAi, N-IR
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
ameliorates  brain cancer
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Under control conditions, the adulthood-only expression of NGD14477 under the control of Scer\GAL4esg-NP7397 (and Gal80[ts], for the temporal regulation of expression) leads to a significant increase in the adult posterior midgut mitotic index in females only, as compared to controls.

Expression of NGD14477 under the control of Scer\GAL4ple.PU as well as the Scer\GAL4elav.PU driver in combination with Scer\GAL80ple.PK (all together with tub-Gal80[ts] to limit the time of expression to adulthood) results in significantly decreased adult lifespan.

Expression of NGD14477 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 does not suppress the age-dependent germline stem cell (GSC) loss seen in wild type females. Although a rapid decline in GSCs is seen at one week after clone induction, by two- to three weeks maintenance in knock-down females is better than that of wild type.

Expression of NGD14477 under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 slows female germline stem cell loss with age.

Expression of NGD14477 under the control of Scer\GAL4bbg-C96 results in frequent serrations along the wing margin.

Expression of NGD14477 under the control of Scer\GAL4dpp.PU results in extra scutellar bristles.

Adults expressing NGD14477 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Depending on the insertion line used, expression under the control of Scer\GAL4Mef2.PR can result in flightless flies or late pupal lethality.

Expression under the control of Scer\GAL4Mef2.PR results in grossly normal adult indirect flight muscles.

Expression under the control of Scer\GAL4Mef2.PR results in morphologically wild-type myofibrils in the adult indirect flight muscles.

Expression under the control of Scer\GAL4Mef2.PR results in wild-type sarcomeres in the adult indirect flight muscles.

Expression under the control of Scer\GAL4pnr-MD237 may result in semi-lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4pnr-MD237 results in extra bristles on the notum in 0% or 0-10% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4pnr-MD237 results in loss of bristles on the notum in 100% or 90-100% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4pnr-MD237 results in the absence of 20-30% or 30-40% of the Scer\GAL4pnr-MD237-expressing area of the notum, depending on the insertion line used.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Suppressor of
NOT Suppressor of
Statement
Reference

NGD14477/Scer\GAL4Mhc.PW is a non-suppressor of visible | progressive phenotype of Pink1B9

Other
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The increased mitotic index in the female adult posterior midgut induced by the adulthood-only expression of NGD14477 under the control of Scer\GAL4esg-NP7397 (and Gal80[ts], for the temporal regulation of expression) is suppressed by the co-expression of SxlKK116156.

The adulthood-only co-expression of NGD14477 and spis.UAS under the control of Scer\GAL4esg-NP7397 (and Gal80[ts], for the temporal regulation of expression) leads to adult posterior midgut hyperplasia in females and males, as compared to controls.

Expression of NGD14477 under the control of Scer\GAL4Mhc.PW does not suppress the abnormal wing posture of Pink1B9 flies.

Inhibition of N activity through NGD14477 suppresses numbTS4D.Scer\UAS induced ectopic neuroblast formation.

Expression of NGD14477 (together with Dcr-2Scer\UAS.cDa) under the control of Scer\GAL4wor.PA in erm1 larval brains leads to a dramatic reduction in ectopic type II neuroblasts compared to erm1 brains alone.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (21)