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General Information
Symbol
Dmel\ND-75GD6309
Species
D. melanogaster
Name
FlyBase ID
FBal0207698
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
ND75 RNAi
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Flies expressing ND75GD6309 under the control of Scer\GAL4da.PU exhibit progressive neurodegeneration: no neuron loss is seen at one day, but significant cortical neuron loss is observed at 10 days old. An increased amount of cell death (caspase activation) is seen compared to controls, and the majority of these cells are neurons rather than glia. The mitochondria of cortical cells are enlarged compared to controls, and both neurons and glia are affected. At 30 days, the number and size of the mitochondria is dramatically increased, sometimes to the extent of almost filling the cytoplasm. Mitochondrial defects observed include honeycombing, effacement of cristae and concentric lamellations of the internal mitochondrial membrane.

When ND75GD6309 is expressed in neurons under the control of Scer\GAL4elav.PU no neuronal loss, vacuolation or mitochondrial enlargement is observed. However the lifespan of these flies is significantly shortened and climbing dysfunction is observed in five day old flies.

When ND75GD6309 is expressed in glia under the control of Scer\GAL4repo.PU neuronal loss, cortical vacuolation and diffuse mitochondrial enlargement are observed. Lifespan is reduced but not climbing defects are seen compared to controls.

Expression of ND75GD6309 in the posterior signalling center (PSC) under the control of either Scer\GAL4Antp-10 leads to an increase in the number of circulating lamellocytes. Differentiation of crystal cells and plasmatocytes is unaffected. Lamellocyte number is unaffected when ND75GD6309 is expressed under the control of Scer\GAL4Lsp2.PH (fat body), Scer\GAL4A58 (epidermis), Scer\GAL4c127 (neurons), Scer\GAL4HCH.Hand (dorsal vessel), Scer\GAL45015 (ring gland), Scer\GAL4ap-md544 (wing disc) or Scer\GAL4btl.PS (trachea). Autonomous expression in the lymph gland progenitor cells under the control of Scer\GAL4dome-PG14 also has no effect on lamellocyte differentiation.

Expression of ND75GD6309 in the posterior signalling center (PSC) under the control of either Scer\GAL4Antp-10 or Scer\GAL4Dot.PK leads to an increase in the number of circulating lamellocytes. The number of PSC cells is unchanged when ND75GD6309 is expressed under the control of Scer\GAL4Antp-10, and no apoptosis is detected.

Adults expressing ND-75GD6309 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
NOT suppressed by
Additional Comments
Genetic Interactions
Statement
Reference

Expression of upd2NIG.5988R does not suppress the increase in circulating lamellocytes seen when ND-75GD6309 is expressed under the control of Scer\GAL4Antp-10.

Expression of upd1NIG.5993R does not suppress the increase in circulating lamellocytes seen when ND-75GD6309 is expressed under the control of Scer\GAL4Antp-10.

Expression of upd3dsRNA.Scer\UAS does not suppress the increase in circulating lamellocytes seen when ND-75GD6309 is expressed under the control of Scer\GAL4Antp-10.

Expression of egrKK103432 does not suppress the increase in circulating lamellocytes seen when ND-75GD6309 is expressed under the control of Scer\GAL4Antp-10.

Expression of egrGD12658 does not suppress the increase in circulating lamellocytes seen when ND-75GD6309 is expressed under the control of Scer\GAL4Antp-10.

Expression of Sod2Scer\UAS.cMa suppresses the increase in circulating lamellocytes seen when ND75GD6309 is expressed under the control of Scer\GAL4Antp-10.

Expression of foxoScer\UAS.cPa suppresses the increase in circulating lamellocytes seen when ND75GD6309 is expressed under the control of Scer\GAL4Dot.PK.

Expression of Akt1GD1361 suppresses the increase in circulating lamellocytes seen when ND75GD6309 is expressed under the control of Scer\GAL4Dot.PK.

Expression of Akt1KK100495 suppresses the increase in circulating lamellocytes seen when ND75GD6309 is expressed under the control of Scer\GAL4Dot.PK.

Expression of spiNIG.10334R suppresses the increase in circulating lamellocytes seen when ND75GD6309 is expressed under the control of Scer\GAL4Antp-10.

Xenogenetic Interactions
Statement
Reference

Down-regulation of ND75 through expression of ND75GD6309 in Scer\GAL4hs.2sev->Mmus\Gria1Lc.Scer\UAS necrotic neurons enhances ROS production and activated JNK signaling.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
ND-75GD6309
ND75GD6309
Name Synonyms
Secondary FlyBase IDs
    References (7)