FlyBase Allele Report: Dmel\Nep1[GD11797]

General Information

Symbol

Dmel\Nep1^GD11797

Species

D. melanogaster

Name

FlyBase ID

FBal0207736

Feature type

allele

Associated gene

Dmel\Nep1

Associated Insertion(s)

Carried in Construct

P{GD11797}

Also Known As

RNAi-Nep1A

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD11797}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD11797

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory | adult stage, with Scer\GAL4^c739

(Turrel et al., 2017)

abnormal memory | adult stage, with Scer\GAL4^VT064246

(Turrel et al., 2017)

female semi-sterile | conditional, with Scer\GAL4^hs.2sev

(Sitnik et al., 2014)

female semi-sterile | conditional, with Scer\GAL4^nSyb.PS

(Sitnik et al., 2014)

female semi-sterile | conditional, with Scer\GAL4^Send1.4kb

(Sitnik et al., 2014)

female semi-sterile | conditional, with Scer\GAL4^Tub.PU

(Sitnik et al., 2014)

lethal, with Scer\GAL4^Tub.PU

(Sitnik et al., 2014)

male semi-sterile | conditional, with Scer\GAL4^hs.2sev

(Sitnik et al., 2014)

male semi-sterile | conditional, with Scer\GAL4^Tub.PU

(Sitnik et al., 2014)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Nep1^GD11797 under the control of either Scer\GAL4^c739 or Scer\GAL4^VT064246 (using tub-Gal80[ts] to limit the expression to adulthood) results in significantly reduced middle-term memory compared to controls (tested in an aversive olfactory memory assay 2hr post-training). Scer\GAL4^VT064246-driven expression also decreases the long-term (24hr) memory.

(Turrel et al., 2017)

Wild type females mated to males expressing Nep1^GD11797 via Scer\GAL4^tub.PU or Scer\GAL4^hs.2sev, using Scer\GAL80^ts.αTub84B to suppress Scer\GAL4 function during development, lay significantly fewer eggs than controls. However, no effect on the proportion of progeny that eclose from laid eggs (hatchability) is observed.

Wild type males mated to females expressing Nep1^GD11797 via Scer\GAL4^tub.PU or Scer\GAL4^hs.2sev, using Scer\GAL80^ts.αTub84B to suppress Scer\GAL4 function during development, lay significantly fewer eggs than controls. No effect on the proportion of overall progeny that eclose from laid eggs (hatchability) is observed, though early hatchability is reduced - the severity is dependent on the insertion used.

Wild type males mated to females expressing Nep1^GD11797 via Scer\GAL4^nSyb.PS or Scer\GAL4^Send1.4kb, using Scer\GAL80^ts.αTub84B to suppress Scer\GAL4 function during development, lay fewer eggs than controls. However, the proportion of progeny that eclose from laid eggs (hatchability) is not affected.

(Sitnik et al., 2014)

Adults expressing Nep1^GD11797 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in viable flies or late pupal lethality.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

11797

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Nep1^GD11797, Scer\GAL4^VT064246 has abnormal memory | adult stage phenotype, enhanceable by Appl^{Aβ.UAS.Tag:HA}, Scer\GAL4^VT064246

(Turrel et al., 2017)

Nep1^GD11797, Scer\GAL4^VT064246 has abnormal memory | adult stage phenotype, enhanceable by Bace^UAS.cCSa, Scer\GAL4^VT064246

(Turrel et al., 2017)

NOT Enhanced by

Statement

Reference

Nep1^GD11797, Scer\GAL4^c739 has abnormal memory | adult stage phenotype, non-enhanceable by Appl^{Aβ.UAS.Tag:HA}, Scer\GAL4^c739

(Turrel et al., 2017)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The impaired middle- or long-term term memory, MTM or LTM, tested in an aversive olfactory memory assay 2hr or 24hr post-training, respectively, characteristic for adult flies expressing Nep1^GD11797 under the control of either Scer\GAL4^c739 or Scer\GAL4^VT064246 (combined with tub-Gal80[ts] to limit expression to adulthood) is not by enhanced by co-expression of Appl^{Aβ.Scer\UAS.T:Ivir\HA1} with the Scer\GAL4^c739 driver, but significantly worsens the MTM phenotype with the Scer\GAL4^VT064246 driver. Flies simultaneously expressing Nep1^GD11797 and Appl^{Aβ.Scer\UAS.T:Ivir\HA1} driven by Scer\GAL4^VT064246 do not show any learning ability defects. Both the MTM and the LTM deficit observed in flies expressing Nep1^GD11797 under Scer\GAL4^VT064246 are exacerbated further by co-expression of Bace^{Scer\UAS.cCSa}.

(Turrel et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v27537

w¹¹¹⁸; P{GD11797}v27537

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

11797

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Nep1^GD11797

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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