Allele Dmel\sxc^GD6968

General Information
Symbol	Dmel\sxc^GD6968	Species	D. melanogaster
Name		FlyBase ID	FBal0207873
Feature type	allele	Associated gene	Dmel\sxc

Map ( GBrowse )
Allele class
Mutagen	in vitro construct - RNAi, in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Dickson et al., 2007.7.18) in vitro construct - RNAi (Dickson et al., 2007.7.18)
Mutations Mapped to the Genome

Type Location Additional Notes References RNAi reagent :1..362
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an inverted repeat. (Dickson et al., 2007.7.18)
Carried in construct	P{GD6968} (Dickson et al., 2007.7.18, Sekine et al., 2010, Mummery-Widmer et al., 2009, Kaasik et al., 2013)
Associated Sequence Features	dsRNA-GD6968
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Scer\GAL4^tub.PU (Sekine et al., 2010) locomotor rhythm defective, with Scer\GAL4^tim.Scer\UAS (Kaasik et al., 2013) partially lethal - majority die \| pupal stage, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) small body \| adult stage, with Scer\GAL4^Ilp2.PR (Sekine et al., 2010) small body \| third instar larval stage, with Scer\GAL4^Ilp2.PR (Sekine et al., 2010) visible, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009)
Phenotype Manifest In
	mesothoracic tergum, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009)
Detailed Description
	Statement Reference Expression of sxc[GD6968] under the control of Scer\GAL4[tim.Scer\UAS] results in shortening of the period of the free-running locomotor activity rhythm in constant darkness. (Kaasik et al., 2013) Third instar larvae and adults expressing sxc[GD6968] under the control of Scer\GAL4[Ilp2.PR] have a significantly reduced body size compared to controls. Expression of sxc[GD6968] under the control of Scer\GAL4[Ilp2.PR] is not overtly toxic to insulin-producing cells (IPCs); the IPC nuclei in third larval instar brains do not show any changes that would be consistent with apoptosis or necrosis. Hemolymph carbohydrate levels are increased in third instar larvae and adults expressing sxc[GD6968] under the control of Scer\GAL4[Ilp2.PR] compared to controls. Expression of sxc[GD6968] under the control of Scer\GAL4[Lsp2.PH] reduces the accumulation of neutral lipids in the fat body compared to controls. (Sekine et al., 2010) Expression under the control of Scer\GAL4[pnr-MD237] may result in semi-lethality, depending on the insertion line used. Expression under the control of Scer\GAL4[pnr-MD237] results in the absence of 0% or 80-90% of the Scer\GAL4[pnr-MD237]-expressing area of the notum, depending on the insertion line used. Expression under the control of Scer\GAL4[pnr-MD237] may result in mild thorax closure defects, depending on the insertion line used. Expression under the control of Scer\GAL4[pnr-MD237] may result in an enlarged notum, depending on the insertion line used. (Mummery-Widmer et al., 2009)
External Data
Linkouts	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 6968
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 2 )
VDRC	v18610 w¹¹¹⁸; P{GD6968}v18610 v18611 w¹¹¹⁸; P{GD6968}v18611
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts
	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 6968
Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Ogt^GD6968 sxc^GD6968
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Kaasik et al., 2013, Cell Metab. 17(2): 291--302 Glucose Sensor O-GlcNAcylation Coordinates with Phosphorylation to Regulate Circadian Clock. [FBrf0220792] Sekine et al., 2010, J. Biol. Chem. 285(49): 38684--38691 Blocking O-Linked GlcNAc Cycling in Drosophila Insulin-producing Cells Perturbs Glucose-Insulin Homeostasis. [FBrf0212389]
Supplementary material	Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]

Allele Dmel\sxcGD6968

Allele Dmel\sxc^GD6968