FlyBase Allele Report: Dmel\Pak3[GD8481]

General Information

Symbol

Dmel\Pak3^GD8481

Species

D. melanogaster

Name

FlyBase ID

FBal0208004

Feature type

allele

Associated gene

Dmel\Pak3

Associated Insertion(s)

Carried in Construct

P{GD8481}

Genomic Maps

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Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

in vitro construct - RNAi

(Dickson et al., 2007.7.18)

in vitro construct - regulatory fusion

(Dickson et al., 2007.7.18)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{GD8481}

Cytology

Nature of the lesion

Statement

Reference

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Pak3

Associated Sequence Features

dsRNA-GD8481

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^ppk.PG

(Hattori et al., 2013)

flightless, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

partially lethal - majority die | adult stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

visible, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

abdominal dorsal multidendritic neuron ddaC | third instar larval stage, with Scer\GAL4^ppk.PG

(Hattori et al., 2013)

dendritic tree | third instar larval stage, with Scer\GAL4^ppk.PG

(Hattori et al., 2013)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

Detailed Description

Statement

Reference

The expression of Pak3^GD8481 under the control of Scer\GAL4^ppk.PG results in a reduced dendritic arborization in third instar larval class IV abdominal dorsal multidendritic ddaC neurons, as compared to controls; expression under the control of Scer\GAL4²²¹ has no obvious effect in third instar larval class I abdominal dorsal multidendritic ddaD and ddaE neurons, as compared to controls.

(Hattori et al., 2013)

Adults expressing Pak3^GD8481 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in flightless flies or defects in wing posture.

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal adult indirect flight muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in morphologically wild-type myofibrils in the adult indirect flight muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in wild-type sarcomeres in the adult indirect flight muscles.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 10-20% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

8481

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Pak3^GD8481, Scer\GAL4^nub.PU is a non-enhancer of visible phenotype of Git^UAS.Tag:MYC, RtGEF^UAS.Tag:HA, Scer\GAL4^nub.PU, hpo^UAS.cUa

(Dent et al., 2015)

Suppressor of

Statement

Reference

Pak3^GD8481, Scer\GAL4²²¹/Scer\GAL4²²¹ is a suppressor | partially of abnormal neuroanatomy phenotype of Scer\GAL4²²¹/Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA}

(Hattori et al., 2013)

Pak3^GD8481, Scer\GAL4^GMR.PU is a suppressor | partially of visible phenotype of Scer\GAL4^GMR.PU, spas^T32

(Ozdowski et al., 2011)

NOT Suppressor of

Statement

Reference

Pak3^GD8481, Scer\GAL4^nub.PU is a non-suppressor of visible phenotype of Git^UAS.Tag:MYC, RtGEF^UAS.Tag:HA, Scer\GAL4^nub.PU, hpo^UAS.cUa

(Dent et al., 2015)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Pak3^GD8481, Scer\GAL4^nub.PU is a non-enhancer of wing phenotype of Git^UAS.Tag:MYC, RtGEF^UAS.Tag:HA, Scer\GAL4^nub.PU, hpo^UAS.cUa

(Dent et al., 2015)

Suppressor of

Statement

Reference

Pak3^GD8481, Scer\GAL4²²¹/Scer\GAL4²²¹ is a suppressor | partially of abdominal dorsal multidendritic neuron ddaD | third instar larval stage phenotype of Scer\GAL4²²¹/Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA}

(Hattori et al., 2013)

Pak3^GD8481, Scer\GAL4²²¹/Scer\GAL4²²¹ is a suppressor | partially of abdominal dorsal multidendritic neuron ddaE | third instar larval stage phenotype of Scer\GAL4²²¹/Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA}

(Hattori et al., 2013)

Pak3^GD8481, Scer\GAL4²²¹/Scer\GAL4²²¹ is a suppressor | partially of dendritic tree | third instar larval stage phenotype of Scer\GAL4²²¹/Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA}

(Hattori et al., 2013)

Pak3^GD8481, Scer\GAL4^GMR.PU is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PU, spas^T32

(Ozdowski et al., 2011)

NOT Suppressor of

Statement

Reference

Pak3^GD8481, Scer\GAL4^nub.PU is a non-suppressor of wing phenotype of Git^UAS.Tag:MYC, RtGEF^UAS.Tag:HA, Scer\GAL4^nub.PU, hpo^UAS.cUa

(Dent et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The strong reduction in wing area seen in flies co-expressing all of hpo^Scer\UAS.cUa, RtGEF^{Scer\UAS.T:Ivir\HA1} and Git^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^nub.PU is unaffected if they are also carrying either Pak3^GD8481.

(Dent et al., 2015)

The increased number of dendrite terminals in third instar larval class I abdominal dorsal multidendritic ddaD and ddaE neurons expressing kn^{Scer\UAS.cHa.T:Ivir\HA1} under the control of Scer\GAL4²²¹ is partially suppressed by co-expressing Pak3^GD8481.

(Hattori et al., 2013)

Expression of Pak3^GD8481 partially suppresses the eye phenotype seen when spas^T32 is expressed under the control of Scer\GAL4^GMR.PU.

(Ozdowski et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (3)