Allele Dmel\Pdf^GD299

General Information
Symbol	Dmel\Pdf^GD299	Species	D. melanogaster
Name		FlyBase ID	FBal0208033
Feature type	allele	Associated gene	Dmel\Pdf

Map ( GBrowse )
Allele class
Mutagen	in vitro construct - RNAi, in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Dickson et al., 2007.7.18) in vitro construct - RNAi (Dickson et al., 2007.7.18)
Mutations Mapped to the Genome

Type Location Additional Notes References RNAi reagent :1..285
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an inverted repeat. (Dickson et al., 2007.7.18)
Carried in construct	P{GD299} (Dickson et al., 2007.7.18, Hermann et al., 2012, Shafer and Taghert, 2009, Mummery-Widmer et al., 2009)
Associated Sequence Features	dsRNA-GD299
Cytology
Phenotypic Data
Phenotypic Class
	body color defective, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) circadian behavior defective, with Scer\GAL4^tim.Scer\UAS (Hermann et al., 2012) partially lethal - majority die \| pupal stage, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009)
Phenotype Manifest In
	mesothoracic tergum, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009)
Detailed Description
	Statement Reference Knockdown of Pdf through expression of Pdf[GD299] under the control of Scer\GAL4[tim.Scer\UAS] results in a clearly advanced phase of the evening activity peak in LD 12:12. (Hermann et al., 2012) Expression under the control of Scer\GAL4[pnr-MD237] may result in semi-lethality, depending on the insertion line used. Expression under the control of Scer\GAL4[pnr-MD237] results in a colour difference between the central Scer\GAL4[pnr-MD237] expression domain of the notum and the surrounding lateral region in 0% or 60-70% of the Scer\GAL4[pnr-MD237] expression domain, depending on the insertion line used. (Mummery-Widmer et al., 2009)
External Data
Linkouts	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 299
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement Reference Dcr-2^Scer\UAS.cDa, Pdf^GD299, Scer\GAL4^Mai179 has circadian behavior defective phenotype (Shafer and Taghert, 2009) Dcr-2^Scer\UAS.cDa, Pdf^GD299, Scer\GAL4^R6 has circadian behavior defective phenotype (Shafer and Taghert, 2009) Dcr-2^Scer\UAS.cDa, Pdf^GD299, Scer\GAL4^tim.Scer\UAS has circadian behavior defective phenotype (Shafer and Taghert, 2009) Pdf^GD299, Scer\GAL4^tim.Scer\UAS, npf^KK111846 has circadian behavior defective phenotype (Hermann et al., 2012)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference In LD 12:12, the evening activity peak of npf[KK111846]/Pdf[GD299] knockdown flies (under the control of Scer\GAL4[tim.Scer\UAS]) is different from Pdf[GD299]-knockdown flies: it appears broader and flatter. (Hermann et al., 2012) Compared to control lines, the Dcr-2[Scer\UAS.cDa]/Scer\GAL4[tim.Scer\UAS]/Pdf[GD299] flies show increased arhythmicity and shortened free-running period under constant darkness. Under light/dark cycles, these flies display reduced morning anticipation and an increase in activity levels in the six hours before lights-off, which results in an advanced evening peak. Dcr-2[Scer\UAS.cDa]/Scer\GAL4[R6]/Pdf[GD299] flies display high levels of arhythmicity under constant darkness. Under light/dark cycles, these flies display significantly reduced morning anticipation controls lacking Scer\GAL4[R6] or Pdf[GD299]. Dcr-2[Scer\UAS.cDa]/Scer\GAL4[Mai179]/Pdf[GD299] flies display high levels of arhythmicity under constant darkness. These flies show a normal free-running period under constant darkness and normally phased evening peak under light/dark cycles. (Shafer and Taghert, 2009)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 2 )
VDRC	v4380 w¹¹¹⁸; P{GD299}v4380 v4382 w¹¹¹⁸; P{GD299}v4382
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts
	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 299
Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Pdf^GD299
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Hermann et al., 2012, J. Comp. Neurol. 520(5): 970--987 Neuropeptide F immunoreactive clock neurons modify evening locomotor activity and free-running period in Drosophila melanogaster. [FBrf0217231] Shafer and Taghert, 2009, PLoS ONE 4(12): e8298 RNA-Interference Knockdown of Drosophila Pigment Dispersing Factor in Neuronal Subsets: The Anatomical Basis of a Neuropeptide's Circadian Functions. [FBrf0209505]
Supplementary material	Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]

Allele Dmel\PdfGD299

Allele Dmel\Pdf^GD299