FlyBase Allele Report: Dmel\Pi3K92E[GD11228]

General Information

Symbol

Dmel\Pi3K92E^GD11228

Species

D. melanogaster

Name

FlyBase ID

FBal0208070

Feature type

allele

Associated gene

Dmel\Pi3K92E

Associated Insertion(s)

Carried in Construct

P{GD11228}

Also Known As

PI3K^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD11228}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD11228

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of glucose metabolism disease

CEA

(Mascolo et al., 2022)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by Ras85D^V12.UAS, dlg1^GD4689

(Willecke et al., 2011)

ameliorates high grade glioma

modeled by Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, btl::Egfr^λ.UAS

(Losada-Pérez et al., 2022)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

chromosome | larval stage, with Scer\GAL4^Act.PU

(Mascolo et al., 2022)

larval neuroblast, with Scer\GAL4^Act.PU

(Mascolo et al., 2022)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Expression of Pi3K92E^GD11228 in an otherwise wild-type posterior wing disc compartment, under the control of Scer\GAL4^en.PU, results in mild growth-suppressing defects.

(Willecke et al., 2011)

Adults expressing Pi3K92E^GD11228 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 40-50% or 50-60% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

11228

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Pi3K92E^GD11228, Scer\GAL4^{FRT.Rnor\Cd2.Act5C} is a suppressor of neoplasia | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Willecke et al., 2011)

Pi3K92E^GD11228, Scer\GAL4^{FRT.Rnor\Cd2.Act5C} is a suppressor of neoplasia | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, dlg1^GD4689

(Willecke et al., 2011)

Pi3K92E^GD11228, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, Scer\GAL80^ts.αTub84B is a suppressor of neoplasia phenotype of Ras85D^V12.UAS, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, Scer\GAL80^ts.αTub84B, dlg1^GD4689

(Willecke et al., 2011)

Pi3K92E^GD11228, Scer\GAL4^en.PU is a suppressor of neoplasia phenotype of Ras85D^V12.UAS, Scer\GAL4^en.PU, dlg1^GD4689

(Willecke et al., 2011)

Phenotype Manifest In

Suppressor of

Statement

Reference

Pi3K92E^GD11228, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, Scer\GAL80^ts.αTub84B is a suppressor of eye phenotype of Ras85D^V12.UAS, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, Scer\GAL80^ts.αTub84B, dlg1^GD4689

(Willecke et al., 2011)

Pi3K92E^GD11228, Scer\GAL4^en.PU is a suppressor of wing disc phenotype of Ras85D^V12.UAS, Scer\GAL4^en.PU, dlg1^GD4689

(Willecke et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Pi3K92E^GD11228 suppresses the tissue overgrowth seen upon expression of yki^Scer\UAS.cHa in the eye imaginal disc.

Knockdown of Pi3K92E in Ras85D^V12.Scer\UAS-dlg1^GD4689 cells, through expression Pi3K92E^GD11228, causes a complete suppression of neoplastic growth and even causes a near-complete absence of mutant eye cells. Only small structures, which contain few cells, remain.

Co-expression of Pi3K92E^GD11228 suppresses the tissue overgrowth seen upon expression of os^Scer\UAS.cZa in the eye imaginal disc.

Co-expression of Pi3K92E^GD11228 suppresses the tissue overgrowth seen upon expression of both yki^Scer\UAS.cHa and os^Scer\UAS.cZa in the eye imaginal disc.

Co-expression of Pi3K92E^GD11228 suppresses the tissue overgrowth seen upon expression of both yki^Scer\UAS.cHa and dlg1^GD4689 in the eye imaginal disc.

Co-expression of Pi3K92E^GD11228 suppresses the tissue overgrowth seen upon expression of both yki^Scer\UAS.cHa and Ras85D^V12.Scer\UAS in the eye imaginal disc.

Co-expression of Pi3K92E^GD11228 together with Ras85D^V12.Scer\UAS and dlg1^GD4689, under the control of Scer\GAL4^en.PU, strongly reduces the size of the Ras85D^V12.Scer\UAS-dlg1^GD4689 tumours in the posterior wing disc compartment. There is an almost complete absence of cellular proliferation in this compartment.

Co-expression of Pi3K92E^GD11228 suppresses tumour growth in Ras85D^V12.Scer\UAS MARCM third instar imaginal disc clones, although this suppression is weaker than that seen upon expression of Pi3K92E^GD11228 in Ras85D^V12.Scer\UAS-dlg1^GD4689 MARCM third instar imaginal disc clones.

Co-expression of Pi3K92E^GD11228 suppresses tumour growth in dlg1^GD4689 MARCM third instar imaginal disc clones, although this suppression is weaker than that seen upon expression of Pi3K92E^GD11228 in Ras85D^V12.Scer\UAS-dlg1^GD4689 MARCM third instar imaginal disc clones.

(Willecke et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v38986

w¹¹¹⁸ P{GD11228}v38986

v38985

w¹¹¹⁸; P{GD11228}v38985

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

11228

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

PI3K92E^RNAi

(Mascolo et al., 2022)

PI3K^RNAi

(Mascolo et al., 2022, Boulan et al., 2019)

Pi3K92E^GD11228

(Im et al., 2018)

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

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