Allele Dmel\Plip^GD4965

General Information
Symbol	Dmel\Plip^GD4965	Species	D. melanogaster
Name		FlyBase ID	FBal0208095
Feature type	allele	Associated gene	Dmel\Plip

Map ( GBrowse )
Allele class
Mutagen	in vitro construct - RNAi, in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Dickson et al., 2007.7.18) in vitro construct - RNAi (Dickson et al., 2007.7.18)
Mutations Mapped to the Genome

Type Location Additional Notes References RNAi reagent :1..311
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an inverted repeat. (Dickson et al., 2007.7.18)
Carried in construct	P{GD4965} (Dickson et al., 2007.7.18, Chang et al., 2011, Neumüller et al., 2011, Mummery-Widmer et al., 2009)
Associated Sequence Features	dsRNA-GD4965
Cytology
Phenotypic Data
Phenotypic Class
	lethal (Neumüller et al., 2011) neuroanatomy defective \| larval stage (Neumüller et al., 2011) viable, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) visible, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009)
Phenotype Manifest In
	embryonic/larval neuroblast \| larval stage (Neumüller et al., 2011) ganglion mother cell \| larval stage (Neumüller et al., 2011) trichogen cell, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009)
Detailed Description
	Statement Reference Expression of Plip[GD4965] under the control of Scer\GAL4[ninaE.PT] has no phenotypic effect on outer photoreceptor cells. (Chang et al., 2011) Expression under the control of Scer\GAL4[1407] results in loss of neuroblasts in the larval brain associated with increased size of the remaining neuroblasts. Expression under the control of Scer\GAL4[1407] results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls. Expression under the control of Scer\GAL4[1407] results in mild defects in the shape of ganglion mother cells in the larval brain. Expression under the control of Scer\GAL4[1407] results in mild defects in the shape of neuroblasts in the larval brain. (Neumüller et al., 2011) Expression under the control of Scer\GAL4[pnr-MD237] results in bristle morphology defects on the notum in 60-70% of the Scer\GAL4[pnr-MD237] expression domain. Expression under the control of Scer\GAL4[pnr-MD237] results in planar polarity defects in the orientation of bristles of the notum in 30-40% of the Scer\GAL4[pnr-MD237] expression domain. Expression under the control of Scer\GAL4[pnr-MD237] results in the absence of 40-50% of the Scer\GAL4[pnr-MD237]-expressing area of the notum. (Mummery-Widmer et al., 2009)
External Data
Linkouts	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 4965 Neuroblasts Screen Database - A database for RNAi phenotypes in larval neural stem cells • 4965
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhancer of
Statement Reference Plip^GD4965, Scer\GAL4^ninaE.PT is a non-enhancer of neuroanatomy defective phenotype of Scer\GAL4^ninaE.PT, TER94^K2A.Scer\UAS (Chang et al., 2011)
Suppressor of
Statement Reference Plip^GD4965, Scer\GAL4^ninaE.PT is a suppressor of neuroanatomy defective phenotype of Scer\GAL4^ninaE.PT, TER94^{A229E.Scer\UAS} (Chang et al., 2011) Plip^GD4965, Scer\GAL4^ninaE.PT is a suppressor of neuroanatomy defective phenotype of Scer\GAL4^ninaE.PT, TER94^{R188Q.Scer\UAS} (Chang et al., 2011)
NOT Suppressor of
Statement Reference Plip^GD4965, Scer\GAL4^ninaE.PT is a non-suppressor of neuroanatomy defective phenotype of Scer\GAL4^ninaE.PT, TER94^K2A.Scer\UAS (Chang et al., 2011)
Phenotype Manifest In
NOT Enhancer of
Statement Reference Plip^GD4965, Scer\GAL4^ninaE.PT is a non-enhancer of outer photoreceptor cell phenotype of Scer\GAL4^ninaE.PT, TER94^K2A.Scer\UAS (Chang et al., 2011)
Suppressor of
Statement Reference Plip^GD4965, Scer\GAL4^ninaE.PT is a suppressor of outer photoreceptor cell phenotype of Scer\GAL4^ninaE.PT, TER94^{A229E.Scer\UAS} (Chang et al., 2011) Plip^GD4965, Scer\GAL4^ninaE.PT is a suppressor of outer photoreceptor cell phenotype of Scer\GAL4^ninaE.PT, TER94^{R188Q.Scer\UAS} (Chang et al., 2011)
NOT Suppressor of
Statement Reference Plip^GD4965, Scer\GAL4^ninaE.PT is a non-suppressor of outer photoreceptor cell phenotype of Scer\GAL4^ninaE.PT, TER94^K2A.Scer\UAS (Chang et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of Plip[GD4965] under the control of Scer\GAL4[ninaE.PT] significantly suppresses the TER94[R188Q.Scer\UAS] photoreceptor cell phenotype. Co-expression of Plip[GD4965] under the control of Scer\GAL4[ninaE.PT] significantly suppresses the TER94[A229E.Scer\UAS] photoreceptor cell phenotype. Co-expression of Plip[GD4965] under the control of Scer\GAL4[ninaE.PT] has no significant effect on TER94[K2A.Scer\UAS]-induced photoreceptor cell degradation. (Chang et al., 2011)
Xenogenetic Interactions
Statement Reference Co-expression of Plip[GD4965] under the control of Scer\GAL4[ninaE.PT] has no significant effect on Hsap\MJD[tr.Q78.Scer\UAS.T:Ivir\HA1]-induced photoreceptor cell degradation. (Chang et al., 2011)
Complementation & Rescue Data
Comments
Stocks ( 3 )
VDRC	v30698 w¹¹¹⁸ P{GD4965}v30698 v47623 w¹¹¹⁸; P{GD4965}v47623 v47624 w¹¹¹⁸; P{GD4965}v47624
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts
	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 4965 Neuroblasts Screen Database - A database for RNAi phenotypes in larval neural stem cells • 4965
Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Plip^GD4965
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Chang et al., 2011, PLoS Genet. 7(2): e1001288 Pathogenic VCP/TER94 Alleles Are Dominant Actives and Contribute to Neurodegeneration by Altering Cellular ATP Level in a Drosophila IBMPFD Model. [FBrf0213008]
Supplementary material	Neumüller et al., 2011, Cell Stem Cell 8(5): Supplemental Table S1. [FBrf0214431] Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]

Allele Dmel\PlipGD4965

Allele Dmel\Plip^GD4965