FlyBase Allele Report: Dmel\PpV[GD7532]

General Information

Symbol

Dmel\PpV^GD7532

Species

D. melanogaster

Name

FlyBase ID

FBal0208115

Feature type

allele

Associated gene

Dmel\PpV

Associated Insertion(s)

Carried in Construct

P{GD7532}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD7532}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD7532

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

lethal - all die during embryonic stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 10-20% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 10-20% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 40-50% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

7532

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

PpV^GD7532, Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C has neoplasia | third instar larval stage | somatic clone phenotype, enhanceable by fmt[+]/fmt¹

(Ma et al., 2017)

Enhancer of

Statement

Reference

PpV^GD7532, Scer\GAL4^GMR.PU is an enhancer of abnormal size | adult stage phenotype of Scer\GAL4^GMR.PU, egr^UAS.cIa

(Ma et al., 2017)

PpV^GD7532, Scer\GAL4^GMR.PU is an enhancer of visible | adult stage phenotype of Scer\GAL4^GMR.PU, egr^UAS.cIa

(Ma et al., 2017)

Other

Statement

Reference

PpV^GD7532, Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C has neoplasia | somatic clone | third instar larval stage phenotype

(Ma et al., 2017)

PpV^GD7532, Scer\GAL4^nub.PU, fmt^GD6928 has visible | adult stage phenotype

(Ma et al., 2017)

PpV^GD7532, Scer\GAL4^nub.PU, fmt^GD6928 has abnormal size | adult stage phenotype

(Ma et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

PpV^GD7532, Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C has eye-antennal disc | third instar larval stage | somatic clone phenotype, enhanceable by fmt[+]/fmt¹

(Ma et al., 2017)

Enhancer of

Statement

Reference

PpV^GD7532, Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Scer\GAL4^GMR.PU, egr^UAS.cIa

(Ma et al., 2017)

Other

Statement

Reference

PpV^GD7532, Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C has eye-antennal disc | somatic clone | third instar larval stage phenotype

(Ma et al., 2017)

PpV^GD7532, Scer\GAL4^nub.PU, fmt^GD6928 has wing phenotype

(Ma et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Ras85D^V12.Scer\UAS and PpV^GD7532 in somatic clones in the eye-antennal disc under the control of Scer\GAL4^Act5C.PI results in mild tumorous overgrowth in third instar larval discs which is further enhanced by fmt¹ heterozygosity.

The small rough eye phenotype characteristic for adult flies expressing egr^Scer\UAS.cIa under the control of Scer\GAL4^GMR.PU is enhanced by co-expression of PpV^GD7532.

Simultaneous expression of both fmt^GD6928 and PpV^GD7532 under the control of Scer\GAL4^nub.PU results in reduced adult wing size, while individual expression of either of the RNAi transgenes has no significant effect on the size of the wing relative to controls.

(Ma et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v31690

w¹¹¹⁸; P{GD7532}v31690

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

7532

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

PpV^GD7532

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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