The expression of PtenGD13500
under the control of Scer\GAL4nub.PU
(together with Dicer-2, for efficient RNAi) leads to the adult wing showing a significant increase in size, associated with an increase in cell number and with a small but significant decrease in cell size, as compared to controls.
The expression for one week during adulthood of PtenGD13500
under the combined control of Scer\GAL4byn-Gal4
, Dicer-2 (for efficient RNAi) and Gal80[ts] (for the temporal control of expression) results in a weak increase in apoptosis in the hindgut, but does not induce dissemination of hindgut cells through the body and does not affect the replication of cells (i.e. BrU-positive) in the pylorus, as compared to controls.
Larvae expressing PtenGD13500
under the control of Scer\GAL4Hml.PG
show reduced encapsulation (15%) of eggs when parasitised by the avirulent L. boulardi wasp strain G486. Plasmatocytes fully cover the egg, while lamellocytes partially cover it.
Expression of PtenGD13500
results in abnormally large wings in which cell size is only moderately affected and cell number is increased.
Adults expressing PtenGD13500
under the control of Scer\GAL4elav.PLu
(in the presence of Dcr-2Scer\UAS.cDa
to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.