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General Information
Symbol
Dmel\PtenGD13500
Species
D. melanogaster
Name
FlyBase ID
FBal0208156
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-PTENRNAi
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UASt regulatory sequences drive expression of an inverted repeat.
Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
The expression of PtenGD13500 under the control of Scer\GAL4nub.PU (together with Dicer-2, for efficient RNAi) leads to the adult wing showing a significant increase in size, associated with an increase in cell number and with a small but significant decrease in cell size, as compared to controls.
The expression for one week during adulthood of PtenGD13500 under the combined control of Scer\GAL4byn-Gal4, Dicer-2 (for efficient RNAi) and Gal80[ts] (for the temporal control of expression) results in a weak increase in apoptosis in the hindgut, but does not induce dissemination of hindgut cells through the body and does not affect the replication of cells (i.e. BrU-positive) in the pylorus, as compared to controls.
Expressing PtenGD13500 under the control of Scer\GAL4GMR.PU leads to a glossy, very rough eye phenotype.
Pupal volume is increased compared to wild-type in animals expressing InRK1409A.Scer\UAS under the control of Scer\GAL4tub.PU.
Larvae expressing PtenGD13500 under the control of Scer\GAL4Hml.PG show reduced encapsulation (15%) of eggs when parasitised by the avirulent L. boulardi wasp strain G486. Plasmatocytes fully cover the egg, while lamellocytes partially cover it.
Expression of PtenGD13500 using Scer\GAL4nub-AC-62 results in abnormally large wings in which cell size is only moderately affected and cell number is increased.
Adults expressing PtenGD13500 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Expression under the control of Scer\GAL4Mef2.PR results in semi-lethality.
Expression under the control of Scer\GAL4pnr-MD237 results in extra bristles on the notum in 10-20% of the Scer\GAL4pnr-MD237 expression domain. Expression under the control of Scer\GAL4pnr-MD237 results in an enlarged notum.
External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
Statement
Reference
Suppressor of
NOT Suppressor of
Other
Statement
Reference
Phenotype Manifest In
Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Enhancer of
Statement
Reference
Suppressor of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference
The double co-expression of PtenGD13500 and SmoxNIG.2262R under the control of Scer\GAL4nub.PU (together with Dicer-2, for efficient RNAi) suppresses the wing defects induced by the single expression conditions.
Quadruple or quintuple co-expression for one week during adulthood of Ras85DV12.cUa.UAS, p53VDRC.cUa, PtenGD13500, ApcVDRC.cUa and/or MedVDRC.cUa under the combined control of Scer\GAL4byn-Gal4, Dicer-2 (for efficient RNAi) and Gal80[ts] (for the temporal control of expression) leads to multilayered epithelia that form bulges at discrete points along the hindgut (only in animals that contained Ras85DV12.cUa.UAS and ApcVDRC.cUa together) and to an expanded pylorus (observed in the quintuple and all quadruple conditions that carried Ras85DV12.cUa.UAS); the quadruple co-expression conditions Ras85DV12.cUa.UAS+p53VDRC.cUa+PtenGD13500+MedVDRC.cUa and p53VDRC.cUa+PtenGD13500+ApcVDRC.cUa+MedVDRC.cUa do not lead to multilayering. All quadruple and quintuple co-expression conditions - with the exception of the quadruple p53VDRC.cUa+PtenGD13500+MedVDRC.cUa+ApcVDRC.cUa - result in numerous hindgut cells losing their characteristic epithelial shape to assume a more mesenchymal appearance (processes extending towards the basement membrane and surrounding muscle layer), migrate locally and disseminate to distant sites in the organism (e.g. head, legs, fat body, ovaries, below the epidermis of the abdomen and within the abdominal cavity). Similar hindgut cell dissemination is observed upon the triple expressions of Ras85DV12.cUa.UAS+p53VDRC.cUa+PtenGD13500, Ras85DV12.cUa.UAS+ApcVDRC.cUa+PtenGD13500 or Ras85DV12.cUa.UAS+p53VDRC.cUa+ApcVDRC.cUa, the double expressions of Ras85DV12.cUa.UAS+p53VDRC.cUa, Ras85DV12.cUa.UAS+PtenGD13500 or Ras85DV12.cUa.UAS+ApcVDRC.cUa, all of which are more severe than upon the single expression of Ras85DV12.cUa.UAS alone. The co-expression for one week during adulthood of PtenGD13500 and ApcVDRC.cUa under the combined control of Scer\GAL4byn-Gal4, Dicer-2 (for efficient RNAi) and Gal80[ts] (for the temporal control of expression) does not affect the replication of cells (i.e. BrU-positive) in the pylorus, as compared to controls. The quadruple co-expression condition p53VDRC.cUa+PtenGD13500+MedVDRC.cUa+ApcVDRC.cUa also leads to a very mild cell dissemination through the body.
Co-expression of hppyScer\UAS.cMa rescues the large wing phenotype of PtenGD13500, Scer\GAL4nub-AC-62 flies. Wing size and the number of cells is more similar to over-expression of hppyScer\UAS.cMa alone, while the cell size phenotype is intermediate.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
PtenGD13500
Name Synonyms
Secondary FlyBase IDs
    References (13)