UASt regulatory sequences drive expression of an inverted repeat.
eye, with Scer\GAL4GMR.PU
The expression of PtenGD13500 under the control of Scer\GAL4nub.PU (together with Dicer-2, for efficient RNAi) leads to the adult wing showing a significant increase in size, associated with an increase in cell number and with a small but significant decrease in cell size, as compared to controls.
The expression for one week during adulthood of PtenGD13500 under the combined control of Scer\GAL4byn-Gal4, Dicer-2 (for efficient RNAi) and Gal80[ts] (for the temporal control of expression) results in a weak increase in apoptosis in the hindgut, but does not induce dissemination of hindgut cells through the body and does not affect the replication of cells (i.e. BrU-positive) in the pylorus, as compared to controls.
Expressing PtenGD13500 under the control of Scer\GAL4GMR.PU leads to a glossy, very rough eye phenotype.
Pupal volume is increased compared to wild-type in animals expressing InRK1409A.Scer\UAS under the control of Scer\GAL4tub.PU.
Larvae expressing PtenGD13500 under the control of Scer\GAL4Hml.PG show reduced encapsulation (15%) of eggs when parasitised by the avirulent L. boulardi wasp strain G486. Plasmatocytes fully cover the egg, while lamellocytes partially cover it.
Expression of PtenGD13500 using Scer\GAL4nub-AC-62 results in abnormally large wings in which cell size is only moderately affected and cell number is increased.
Adults expressing PtenGD13500 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Expression under the control of Scer\GAL4Mef2.PR results in semi-lethality.
Expression under the control of Scer\GAL4pnr-MD237 results in extra bristles on the notum in 10-20% of the Scer\GAL4pnr-MD237 expression domain.
Expression under the control of Scer\GAL4pnr-MD237 results in an enlarged notum.
PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4 has increased occurrence of cell division | adult stage phenotype, enhanceable by ApcVDRC.cUa, Scer\GAL4byn-Gal4
ApcVDRC.cUa, PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4, p53VDRC.cUa has cell migration defective | adult stage phenotype, enhanceable by Tsc1TRiP.cUa, Scer\GAL4byn-Gal4
ApcVDRC.cUa, PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4, p53VDRC.cUa has cell migration defective | adult stage phenotype, enhanceable by gigVDRC.cUa, Scer\GAL4byn-Gal4
PtenGD13500, Scer\GAL4nub.PU has size defective | adult stage phenotype, suppressible | partially by SmoxNIG.2262R, Scer\GAL4nub.PU
PtenGD13500, Scer\GAL4nub.PU has decreased cell size | adult stage phenotype, suppressible by SmoxNIG.2262R, Scer\GAL4nub.PU
PtenGD13500, Scer\GAL4nub.PU has increased cell number | adult stage phenotype, suppressible by SmoxNIG.2262R, Scer\GAL4nub.PU
PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4 has increased occurrence of cell division | adult stage phenotype, suppressible | partially by p53VDRC.cUa, Scer\GAL4byn-Gal4
PtenGD13500, Scer\GAL4nub-AC-62 has size defective phenotype, suppressible by hppyUAS.cMa, Scer\GAL4nub-AC-62
PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4 has decreased occurrence of cell division | adult stage phenotype, non-suppressible by ApcVDRC.cUa, Scer\GAL4byn-Gal4
PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4 has decreased occurrence of cell division | adult stage phenotype, non-suppressible by p53VDRC.cUa, Scer\GAL4byn-Gal4
MedVDRC.cUa, ApcVDRC.cUa, p53VDRC.cUa, PtenGD13500, Scer\GAL4byn-Gal4 is an enhancer of cell migration defective | adult stage phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
PtenGD13500, Scer\GAL4byn-Gal4 is an enhancer of increased occurrence of cell division | adult stage phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
PtenGD13500, Scer\GAL4byn-Gal4 is an enhancer of cell migration defective | adult stage phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
PtenGD13500, Scer\GAL4byn-Gal4 is an enhancer of increased occurrence of cell division | adult stage phenotype of ApcVDRC.cUa, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
Scer\GAL4byn-Gal4, ApcVDRC.cUa/PtenGD13500 is an enhancer of cell migration defective | adult stage phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
p53VDRC.cUa/PtenGD13500, Scer\GAL4byn-Gal4 is an enhancer of cell migration defective | adult stage phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
Scer\GAL4byn-Gal4, ApcVDRC.cUa, p53VDRC.cUa, PtenGD13500 is an enhancer of cell migration defective | adult stage phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
Scer\GAL4byn-Gal4, MedVDRC.cUa, p53VDRC.cUa, PtenGD13500 is an enhancer of cell migration defective | adult stage phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
MedVDRC.cUa, ApcVDRC.cUa, PtenGD13500, Scer\GAL4byn-Gal4 is an enhancer of cell migration defective | adult stage phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
PtenGD13500, Scer\GAL4nub.PU is a suppressor of size defective | adult stage phenotype of Scer\GAL4nub.PU, SmoxNIG.2262R
PtenGD13500, Scer\GAL4nub.PU is a suppressor of increased cell size | adult stage phenotype of Scer\GAL4nub.PU, SmoxNIG.2262R
PtenGD13500, Scer\GAL4nub.PU is a suppressor of decreased cell number | adult stage phenotype of Scer\GAL4nub.PU, SmoxNIG.2262R
Scer\GAL4byn-Gal4, ApcVDRC.cUa, p53VDRC.cUa, PtenGD13500 is a suppressor | partially of decreased occurrence of cell division | adult stage phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
p53VDRC.cUa/PtenGD13500, Scer\GAL4byn-Gal4 is a suppressor of increased cell death | adult stage phenotype of ApcVDRC.cUa, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
PtenGD13500, Scer\GAL4byn-Gal4 is a suppressor | partially of increased cell death | adult stage phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
Scer\GAL4byn-Gal4, ApcVDRC.cUa, p53VDRC.cUa, PtenGD13500 is a suppressor of increased cell death | adult stage phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
PtenGD13500, Scer\GAL4byn-Gal4 is a non-suppressor of decreased occurrence of cell division | adult stage phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
ApcVDRC.cUa, MedVDRC.cUa, PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4, p53VDRC.cUa has cell shape defective | adult stage phenotype
ApcVDRC.cUa, MedVDRC.cUa, PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4, p53VDRC.cUa has short lived phenotype
MedVDRC.cUa, PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4, p53VDRC.cUa has hyperplasia | adult stage phenotype
ApcVDRC.cUa, MedVDRC.cUa, PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4 has cell shape defective | adult stage phenotype
ApcVDRC.cUa, MedVDRC.cUa, PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4 has short lived phenotype
ApcVDRC.cUa, MedVDRC.cUa, PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4 has hyperplasia | adult stage phenotype
ApcVDRC.cUa, MedVDRC.cUa, PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4 has tumorigenic phenotype
ApcVDRC.cUa, MedVDRC.cUa, PtenGD13500, Scer\GAL4byn-Gal4, p53VDRC.cUa has hyperplasia | adult stage phenotype
ApcVDRC.cUa, MedVDRC.cUa, PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4, p53VDRC.cUa has hyperplasia | adult stage phenotype
ApcVDRC.cUa, MedVDRC.cUa, PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4, p53VDRC.cUa has tumorigenic phenotype
ApcVDRC.cUa, PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4, p53VDRC.cUa has cell shape defective | adult stage phenotype
ApcVDRC.cUa, PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4, p53VDRC.cUa has hyperplasia | adult stage phenotype
ApcVDRC.cUa, PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4, p53VDRC.cUa has tumorigenic phenotype
PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4 has adult pyloric epithelium phenotype, enhanceable by ApcVDRC.cUa, Scer\GAL4byn-Gal4
ApcVDRC.cUa, PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4, p53VDRC.cUa has adult hindgut epithelium phenotype, enhanceable by Tsc1TRiP.cUa, Scer\GAL4byn-Gal4
ApcVDRC.cUa, PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4, p53VDRC.cUa has adult hindgut epithelium phenotype, enhanceable by gigVDRC.cUa, Scer\GAL4byn-Gal4
PtenGD13500, Scer\GAL4nub.PU has wing phenotype, suppressible | partially by SmoxNIG.2262R, Scer\GAL4nub.PU
PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4 has adult hindgut epithelium | adult stage phenotype, non-suppressible by ApcVDRC.cUa, Scer\GAL4byn-Gal4
PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4 has adult hindgut epithelium | adult stage phenotype, non-suppressible by p53VDRC.cUa, Scer\GAL4byn-Gal4
MedVDRC.cUa, ApcVDRC.cUa, p53VDRC.cUa, PtenGD13500, Scer\GAL4byn-Gal4 is an enhancer of adult hindgut phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
MedVDRC.cUa, ApcVDRC.cUa, PtenGD13500, Scer\GAL4byn-Gal4 is an enhancer of adult hindgut phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
MedVDRC.cUa, ApcVDRC.cUa, PtenGD13500, Scer\GAL4byn-Gal4 is an enhancer of adult hindgut epithelium phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
PtenGD13500, Scer\GAL4byn-Gal4 is an enhancer of adult hindgut phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
PtenGD13500, Scer\GAL4byn-Gal4 is an enhancer of adult hindgut epithelium phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
PtenGD13500, Scer\GAL4byn-Gal4 is an enhancer of adult pyloric epithelium phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
MedVDRC.cUa, ApcVDRC.cUa, p53VDRC.cUa, PtenGD13500, Scer\GAL4byn-Gal4 is an enhancer of adult hindgut epithelium phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
Scer\GAL4byn-Gal4, ApcVDRC.cUa/PtenGD13500 is an enhancer of adult hindgut phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
Scer\GAL4byn-Gal4, ApcVDRC.cUa/PtenGD13500 is an enhancer of adult hindgut epithelium phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
PtenGD13500, Scer\GAL4byn-Gal4 is an enhancer of adult pyloric epithelium phenotype of ApcVDRC.cUa, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
p53VDRC.cUa/PtenGD13500, Scer\GAL4byn-Gal4 is an enhancer of adult hindgut phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
p53VDRC.cUa/PtenGD13500, Scer\GAL4byn-Gal4 is an enhancer of adult hindgut epithelium phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
Scer\GAL4byn-Gal4, ApcVDRC.cUa, p53VDRC.cUa, PtenGD13500 is an enhancer of adult hindgut phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
Scer\GAL4byn-Gal4, ApcVDRC.cUa, p53VDRC.cUa, PtenGD13500 is an enhancer of adult hindgut epithelium phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
Scer\GAL4byn-Gal4, MedVDRC.cUa, p53VDRC.cUa, PtenGD13500 is an enhancer of adult hindgut phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
Scer\GAL4byn-Gal4, MedVDRC.cUa, p53VDRC.cUa, PtenGD13500 is an enhancer of adult hindgut epithelium phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
PtenGD13500, Scer\GAL4nub.PU is a suppressor of wing phenotype of Scer\GAL4nub.PU, SmoxNIG.2262R
p53VDRC.cUa/PtenGD13500, Scer\GAL4byn-Gal4 is a suppressor of adult hindgut epithelium | adult stage phenotype of ApcVDRC.cUa, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
PtenGD13500, Scer\GAL4byn-Gal4 is a suppressor | partially of adult hindgut epithelium | adult stage phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
p53VDRC.cUa/PtenGD13500, Scer\GAL4byn-Gal4 is a suppressor | partially of adult hindgut epithelium phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
PtenGD13500, Scer\GAL4byn-Gal4 is a non-suppressor | partially of adult hindgut epithelium | adult stage phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4
MedVDRC.cUa, PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4, p53VDRC.cUa has adult pylorus phenotype
ApcVDRC.cUa, MedVDRC.cUa, PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4 has adult pylorus phenotype
ApcVDRC.cUa, MedVDRC.cUa, PtenGD13500, Scer\GAL4byn-Gal4, p53VDRC.cUa has adult pylorus phenotype
ApcVDRC.cUa, MedVDRC.cUa, PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4, p53VDRC.cUa has adult pylorus phenotype
ApcVDRC.cUa, PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4, p53VDRC.cUa has adult pylorus phenotype
The double co-expression of PtenGD13500 and SmoxNIG.2262R under the control of Scer\GAL4nub.PU (together with Dicer-2, for efficient RNAi) suppresses the wing defects induced by the single expression conditions.
Quadruple or quintuple co-expression for one week during adulthood of Ras85DV12.cUa.UAS, p53VDRC.cUa, PtenGD13500, ApcVDRC.cUa and/or MedVDRC.cUa under the combined control of Scer\GAL4byn-Gal4, Dicer-2 (for efficient RNAi) and Gal80[ts] (for the temporal control of expression) leads to multilayered epithelia that form bulges at discrete points along the hindgut (only in animals that contained Ras85DV12.cUa.UAS and ApcVDRC.cUa together) and to an expanded pylorus (observed in the quintuple and all quadruple conditions that carried Ras85DV12.cUa.UAS); the quadruple co-expression conditions Ras85DV12.cUa.UAS+p53VDRC.cUa+PtenGD13500+MedVDRC.cUa and p53VDRC.cUa+PtenGD13500+ApcVDRC.cUa+MedVDRC.cUa do not lead to multilayering.
All quadruple and quintuple co-expression conditions - with the exception of the quadruple p53VDRC.cUa+PtenGD13500+MedVDRC.cUa+ApcVDRC.cUa - result in numerous hindgut cells losing their characteristic epithelial shape to assume a more mesenchymal appearance (processes extending towards the basement membrane and surrounding muscle layer), migrate locally and disseminate to distant sites in the organism (e.g. head, legs, fat body, ovaries, below the epidermis of the abdomen and within the abdominal cavity). Similar hindgut cell dissemination is observed upon the triple expressions of Ras85DV12.cUa.UAS+p53VDRC.cUa+PtenGD13500, Ras85DV12.cUa.UAS+ApcVDRC.cUa+PtenGD13500 or Ras85DV12.cUa.UAS+p53VDRC.cUa+ApcVDRC.cUa, the double expressions of Ras85DV12.cUa.UAS+p53VDRC.cUa, Ras85DV12.cUa.UAS+PtenGD13500 or Ras85DV12.cUa.UAS+ApcVDRC.cUa, all of which are more severe than upon the single expression of Ras85DV12.cUa.UAS alone.
The co-expression for one week during adulthood of PtenGD13500 and ApcVDRC.cUa under the combined control of Scer\GAL4byn-Gal4, Dicer-2 (for efficient RNAi) and Gal80[ts] (for the temporal control of expression) does not affect the replication of cells (i.e. BrU-positive) in the pylorus, as compared to controls.
The quadruple co-expression condition p53VDRC.cUa+PtenGD13500+MedVDRC.cUa+ApcVDRC.cUa also leads to a very mild cell dissemination through the body.
Co-expression of hppyScer\UAS.cMa rescues the large wing phenotype of PtenGD13500, Scer\GAL4nub-AC-62 flies. Wing size and the number of cells is more similar to over-expression of hppyScer\UAS.cMa alone, while the cell size phenotype is intermediate.