FlyBase Allele Report: Dmel\Pten[GD13500]

General Information

Symbol

Dmel\Pten^GD13500

Species

D. melanogaster

Name

FlyBase ID

FBal0208156

Feature type

allele

Associated gene

Dmel\Pten

Associated Insertion(s)

Carried in Construct

P{GD13500}

Also Known As

UAS-PTEN^RNAi, UAS-Pten-i

Genomic Maps

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Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

in vitro construct - RNAi

(Dickson et al., 2007.7.18)

in vitro construct - regulatory fusion

(Dickson et al., 2007.7.18)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{GD13500}

Cytology

Nature of the lesion

Statement

Reference

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Pten

Associated Sequence Features

dsRNA-GD13500

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of colorectal cancer

CEA with p53^VDRC.cUa, Ras85D^V12.cUa.UAS, Apc^VDRC.cUa, Med^VDRC.cUa

(Bangi et al., 2016)

CEA with p53^VDRC.cUa, Ras85D^V12.cUa.UAS, Apc^VDRC.cUa

(Bangi et al., 2016)

CEA with Ras85D^V12.cUa.UAS, Apc^VDRC.cUa, Med^VDRC.cUa

(Bangi et al., 2016)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

decreased cell size | adult stage, with Scer\GAL4^nub.PU

(Hevia et al., 2017)

immune response defective | larval stage, with Scer\GAL4^Hml.PG

(Howell et al., 2012)

increased cell death | adult stage, with Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

increased cell number, with Scer\GAL4^nub-AC-62

(Resnik-Docampo and de Celis, 2011)

increased cell number | adult stage, with Scer\GAL4^nub.PU

(Hevia et al., 2017)

large body | pupal stage, with Scer\GAL4^Tub.PU

(Okamoto et al., 2013)

partially lethal, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

partially lethal - majority die, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

size defective, with Scer\GAL4^nub-AC-62

(Resnik-Docampo and de Celis, 2011)

size defective | adult stage, with Scer\GAL4^nub.PU

(Hevia et al., 2017)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^GMR.PU

(Iyer et al., 2016)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

adult hindgut | adult stage, with Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

chaeta | supernumerary, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

eye, with Scer\GAL4^GMR.PU

(Iyer et al., 2016)

mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing, with Scer\GAL4^nub.PU

(Hevia et al., 2017)

wing, with Scer\GAL4^nub-AC-62

(Resnik-Docampo and de Celis, 2011)

Detailed Description

Statement

Reference

The expression of Pten^GD13500 under the control of Scer\GAL4^nub.PU (together with Dicer-2, for efficient RNAi) leads to the adult wing showing a significant increase in size, associated with an increase in cell number and with a small but significant decrease in cell size, as compared to controls.

(Hevia et al., 2017)

The expression for one week during adulthood of Pten^GD13500 under the combined control of Scer\GAL4^byn-Gal4, Dicer-2 (for efficient RNAi) and Gal80[ts] (for the temporal control of expression) results in a weak increase in apoptosis in the hindgut, but does not induce dissemination of hindgut cells through the body and does not affect the replication of cells (i.e. BrU-positive) in the pylorus, as compared to controls.

(Bangi et al., 2016)

Expressing Pten^GD13500 under the control of Scer\GAL4^GMR.PU leads to a glossy, very rough eye phenotype.

(Iyer et al., 2016)

Pupal volume is increased compared to wild-type in animals expressing InR^{K1409A.Scer\UAS} under the control of Scer\GAL4^tub.PU.

(Okamoto et al., 2013)

Larvae expressing Pten^GD13500 under the control of Scer\GAL4^Hml.PG show reduced encapsulation (15%) of eggs when parasitised by the avirulent L. boulardi wasp strain G486. Plasmatocytes fully cover the egg, while lamellocytes partially cover it.

(Howell et al., 2012)

Expression of Pten^GD13500 using Scer\GAL4^nub-AC-62 results in abnormally large wings in which cell size is only moderately affected and cell number is increased.

(Resnik-Docampo and de Celis, 2011)

Adults expressing Pten^GD13500 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in semi-lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in extra bristles on the notum in 10-20% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in an enlarged notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

13500

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Pten^GD13500, Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4 has increased occurrence of cell division | adult stage phenotype, enhanceable by Apc^VDRC.cUa, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Apc^VDRC.cUa, Pten^GD13500, Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4, p53^VDRC.cUa has cell migration defective | adult stage phenotype, enhanceable by Tsc1^TRiP.cUa, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Apc^VDRC.cUa, Pten^GD13500, Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4, p53^VDRC.cUa has cell migration defective | adult stage phenotype, enhanceable by gig^VDRC.cUa, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Suppressed by

Statement

Reference

Pten^GD13500, Scer\GAL4^nub.PU has size defective | adult stage phenotype, suppressible | partially by Smox^NIG.2262R, Scer\GAL4^nub.PU

(Hevia et al., 2017)

Pten^GD13500, Scer\GAL4^nub.PU has decreased cell size | adult stage phenotype, suppressible by Smox^NIG.2262R, Scer\GAL4^nub.PU

(Hevia et al., 2017)

Pten^GD13500, Scer\GAL4^nub.PU has increased cell number | adult stage phenotype, suppressible by Smox^NIG.2262R, Scer\GAL4^nub.PU

(Hevia et al., 2017)

Pten^GD13500, Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4 has increased occurrence of cell division | adult stage phenotype, suppressible | partially by p53^VDRC.cUa, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Pten^GD13500, Scer\GAL4^nub-AC-62 has size defective phenotype, suppressible by hppy^UAS.cMa, Scer\GAL4^nub-AC-62

(Resnik-Docampo and de Celis, 2011)

NOT suppressed by

Statement

Reference

Pten^GD13500, Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4 has decreased occurrence of cell division | adult stage phenotype, non-suppressible by Apc^VDRC.cUa, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Pten^GD13500, Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4 has decreased occurrence of cell division | adult stage phenotype, non-suppressible by p53^VDRC.cUa, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Enhancer of

Statement

Reference

Med^VDRC.cUa, Apc^VDRC.cUa, p53^VDRC.cUa, Pten^GD13500, Scer\GAL4^byn-Gal4 is an enhancer of cell migration defective | adult stage phenotype of Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Pten^GD13500, Scer\GAL4^byn-Gal4 is an enhancer of increased occurrence of cell division | adult stage phenotype of Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Pten^GD13500, Scer\GAL4^byn-Gal4 is an enhancer of cell migration defective | adult stage phenotype of Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Pten^GD13500, Scer\GAL4^byn-Gal4 is an enhancer of increased occurrence of cell division | adult stage phenotype of Apc^VDRC.cUa, Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Scer\GAL4^byn-Gal4, Apc^VDRC.cUa/Pten^GD13500 is an enhancer of cell migration defective | adult stage phenotype of Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

p53^VDRC.cUa/Pten^GD13500, Scer\GAL4^byn-Gal4 is an enhancer of cell migration defective | adult stage phenotype of Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Scer\GAL4^byn-Gal4, Apc^VDRC.cUa, p53^VDRC.cUa, Pten^GD13500 is an enhancer of cell migration defective | adult stage phenotype of Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Scer\GAL4^byn-Gal4, Med^VDRC.cUa, p53^VDRC.cUa, Pten^GD13500 is an enhancer of cell migration defective | adult stage phenotype of Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Med^VDRC.cUa, Apc^VDRC.cUa, Pten^GD13500, Scer\GAL4^byn-Gal4 is an enhancer of cell migration defective | adult stage phenotype of Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Suppressor of

Statement

Reference

Pten^GD13500, Scer\GAL4^nub.PU is a suppressor of size defective | adult stage phenotype of Scer\GAL4^nub.PU, Smox^NIG.2262R

(Hevia et al., 2017)

Pten^GD13500, Scer\GAL4^nub.PU is a suppressor of increased cell size | adult stage phenotype of Scer\GAL4^nub.PU, Smox^NIG.2262R

(Hevia et al., 2017)

Pten^GD13500, Scer\GAL4^nub.PU is a suppressor of decreased cell number | adult stage phenotype of Scer\GAL4^nub.PU, Smox^NIG.2262R

(Hevia et al., 2017)

Scer\GAL4^byn-Gal4, Apc^VDRC.cUa, p53^VDRC.cUa, Pten^GD13500 is a suppressor | partially of decreased occurrence of cell division | adult stage phenotype of Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

p53^VDRC.cUa/Pten^GD13500, Scer\GAL4^byn-Gal4 is a suppressor of increased cell death | adult stage phenotype of Apc^VDRC.cUa, Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Pten^GD13500, Scer\GAL4^byn-Gal4 is a suppressor | partially of increased cell death | adult stage phenotype of Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Scer\GAL4^byn-Gal4, Apc^VDRC.cUa, p53^VDRC.cUa, Pten^GD13500 is a suppressor of increased cell death | adult stage phenotype of Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

NOT Suppressor of

Statement

Reference

Pten^GD13500, Scer\GAL4^byn-Gal4 is a non-suppressor of decreased occurrence of cell division | adult stage phenotype of Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Other

Statement

Reference

Apc^VDRC.cUa, Med^VDRC.cUa, Pten^GD13500, Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4, p53^VDRC.cUa has cell shape defective | adult stage phenotype

(Bangi et al., 2016)

Apc^VDRC.cUa, Med^VDRC.cUa, Pten^GD13500, Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4, p53^VDRC.cUa has short lived phenotype

(Bangi et al., 2016)

Med^VDRC.cUa, Pten^GD13500, Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4, p53^VDRC.cUa has hyperplasia | adult stage phenotype

(Bangi et al., 2016)

Apc^VDRC.cUa, Med^VDRC.cUa, Pten^GD13500, Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4 has cell shape defective | adult stage phenotype

(Bangi et al., 2016)

Apc^VDRC.cUa, Med^VDRC.cUa, Pten^GD13500, Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4 has short lived phenotype

(Bangi et al., 2016)

Apc^VDRC.cUa, Med^VDRC.cUa, Pten^GD13500, Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4 has hyperplasia | adult stage phenotype

(Bangi et al., 2016)

Apc^VDRC.cUa, Med^VDRC.cUa, Pten^GD13500, Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4 has tumorigenic phenotype

(Bangi et al., 2016)

Apc^VDRC.cUa, Med^VDRC.cUa, Pten^GD13500, Scer\GAL4^byn-Gal4, p53^VDRC.cUa has hyperplasia | adult stage phenotype

(Bangi et al., 2016)

Apc^VDRC.cUa, Med^VDRC.cUa, Pten^GD13500, Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4, p53^VDRC.cUa has hyperplasia | adult stage phenotype

(Bangi et al., 2016)

Apc^VDRC.cUa, Med^VDRC.cUa, Pten^GD13500, Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4, p53^VDRC.cUa has tumorigenic phenotype

(Bangi et al., 2016)

Apc^VDRC.cUa, Pten^GD13500, Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4, p53^VDRC.cUa has cell shape defective | adult stage phenotype

(Bangi et al., 2016)

Apc^VDRC.cUa, Pten^GD13500, Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4, p53^VDRC.cUa has hyperplasia | adult stage phenotype

(Bangi et al., 2016)

Apc^VDRC.cUa, Pten^GD13500, Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4, p53^VDRC.cUa has tumorigenic phenotype

(Bangi et al., 2016)

Med^VDRC.cUa, Pten^GD13500, Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4, p53^VDRC.cUa has cell shape defective | adult stage phenotype

(Bangi et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

Pten^GD13500, Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4 has adult pyloric epithelium phenotype, enhanceable by Apc^VDRC.cUa, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Apc^VDRC.cUa, Pten^GD13500, Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4, p53^VDRC.cUa has adult hindgut epithelium phenotype, enhanceable by Tsc1^TRiP.cUa, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Apc^VDRC.cUa, Pten^GD13500, Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4, p53^VDRC.cUa has adult hindgut epithelium phenotype, enhanceable by gig^VDRC.cUa, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Suppressed by

Statement

Reference

Pten^GD13500, Scer\GAL4^nub.PU has wing phenotype, suppressible | partially by Smox^NIG.2262R, Scer\GAL4^nub.PU

(Hevia et al., 2017)

NOT suppressed by

Statement

Reference

Pten^GD13500, Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4 has adult hindgut epithelium | adult stage phenotype, non-suppressible by Apc^VDRC.cUa, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Pten^GD13500, Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4 has adult hindgut epithelium | adult stage phenotype, non-suppressible by p53^VDRC.cUa, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Enhancer of

Statement

Reference

Med^VDRC.cUa, Apc^VDRC.cUa, p53^VDRC.cUa, Pten^GD13500, Scer\GAL4^byn-Gal4 is an enhancer of adult hindgut phenotype of Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Med^VDRC.cUa, Apc^VDRC.cUa, Pten^GD13500, Scer\GAL4^byn-Gal4 is an enhancer of adult hindgut phenotype of Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Med^VDRC.cUa, Apc^VDRC.cUa, Pten^GD13500, Scer\GAL4^byn-Gal4 is an enhancer of adult hindgut epithelium phenotype of Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Pten^GD13500, Scer\GAL4^byn-Gal4 is an enhancer of adult hindgut phenotype of Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Pten^GD13500, Scer\GAL4^byn-Gal4 is an enhancer of adult hindgut epithelium phenotype of Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Pten^GD13500, Scer\GAL4^byn-Gal4 is an enhancer of adult pyloric epithelium phenotype of Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Med^VDRC.cUa, Apc^VDRC.cUa, p53^VDRC.cUa, Pten^GD13500, Scer\GAL4^byn-Gal4 is an enhancer of adult hindgut epithelium phenotype of Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Scer\GAL4^byn-Gal4, Apc^VDRC.cUa/Pten^GD13500 is an enhancer of adult hindgut phenotype of Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Scer\GAL4^byn-Gal4, Apc^VDRC.cUa/Pten^GD13500 is an enhancer of adult hindgut epithelium phenotype of Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Pten^GD13500, Scer\GAL4^byn-Gal4 is an enhancer of adult pyloric epithelium phenotype of Apc^VDRC.cUa, Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

p53^VDRC.cUa/Pten^GD13500, Scer\GAL4^byn-Gal4 is an enhancer of adult hindgut phenotype of Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

p53^VDRC.cUa/Pten^GD13500, Scer\GAL4^byn-Gal4 is an enhancer of adult hindgut epithelium phenotype of Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Scer\GAL4^byn-Gal4, Apc^VDRC.cUa, p53^VDRC.cUa, Pten^GD13500 is an enhancer of adult hindgut phenotype of Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Scer\GAL4^byn-Gal4, Apc^VDRC.cUa, p53^VDRC.cUa, Pten^GD13500 is an enhancer of adult hindgut epithelium phenotype of Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Scer\GAL4^byn-Gal4, Med^VDRC.cUa, p53^VDRC.cUa, Pten^GD13500 is an enhancer of adult hindgut phenotype of Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Scer\GAL4^byn-Gal4, Med^VDRC.cUa, p53^VDRC.cUa, Pten^GD13500 is an enhancer of adult hindgut epithelium phenotype of Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Suppressor of

Statement

Reference

Pten^GD13500, Scer\GAL4^nub.PU is a suppressor of wing phenotype of Scer\GAL4^nub.PU, Smox^NIG.2262R

(Hevia et al., 2017)

p53^VDRC.cUa/Pten^GD13500, Scer\GAL4^byn-Gal4 is a suppressor of adult hindgut epithelium | adult stage phenotype of Apc^VDRC.cUa, Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Pten^GD13500, Scer\GAL4^byn-Gal4 is a suppressor | partially of adult hindgut epithelium | adult stage phenotype of Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

p53^VDRC.cUa/Pten^GD13500, Scer\GAL4^byn-Gal4 is a suppressor | partially of adult hindgut epithelium phenotype of Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

NOT Suppressor of

Statement

Reference

Pten^GD13500, Scer\GAL4^byn-Gal4 is a non-suppressor | partially of adult hindgut epithelium | adult stage phenotype of Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4

(Bangi et al., 2016)

Other

Statement

Reference

Med^VDRC.cUa, Pten^GD13500, Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4, p53^VDRC.cUa has adult pylorus phenotype

(Bangi et al., 2016)

Apc^VDRC.cUa, Med^VDRC.cUa, Pten^GD13500, Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4 has adult pylorus phenotype

(Bangi et al., 2016)

Apc^VDRC.cUa, Med^VDRC.cUa, Pten^GD13500, Scer\GAL4^byn-Gal4, p53^VDRC.cUa has adult pylorus phenotype

(Bangi et al., 2016)

Apc^VDRC.cUa, Med^VDRC.cUa, Pten^GD13500, Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4, p53^VDRC.cUa has adult pylorus phenotype

(Bangi et al., 2016)

Apc^VDRC.cUa, Pten^GD13500, Ras85D^V12.cUa.UAS, Scer\GAL4^byn-Gal4, p53^VDRC.cUa has adult pylorus phenotype

(Bangi et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The double co-expression of Pten^GD13500 and Smox^NIG.2262R under the control of Scer\GAL4^nub.PU (together with Dicer-2, for efficient RNAi) suppresses the wing defects induced by the single expression conditions.

(Hevia et al., 2017)

Quadruple or quintuple co-expression for one week during adulthood of Ras85D^V12.cUa.UAS, p53^VDRC.cUa, Pten^GD13500, Apc^VDRC.cUa and/or Med^VDRC.cUa under the combined control of Scer\GAL4^byn-Gal4, Dicer-2 (for efficient RNAi) and Gal80[ts] (for the temporal control of expression) leads to multilayered epithelia that form bulges at discrete points along the hindgut (only in animals that contained Ras85D^V12.cUa.UAS and Apc^VDRC.cUa together) and to an expanded pylorus (observed in the quintuple and all quadruple conditions that carried Ras85D^V12.cUa.UAS); the quadruple co-expression conditions Ras85D^V12.cUa.UAS+p53^VDRC.cUa+Pten^GD13500+Med^VDRC.cUa and p53^VDRC.cUa+Pten^GD13500+Apc^VDRC.cUa+Med^VDRC.cUa do not lead to multilayering.

All quadruple and quintuple co-expression conditions - with the exception of the quadruple p53^VDRC.cUa+Pten^GD13500+Med^VDRC.cUa+Apc^VDRC.cUa - result in numerous hindgut cells losing their characteristic epithelial shape to assume a more mesenchymal appearance (processes extending towards the basement membrane and surrounding muscle layer), migrate locally and disseminate to distant sites in the organism (e.g. head, legs, fat body, ovaries, below the epidermis of the abdomen and within the abdominal cavity). Similar hindgut cell dissemination is observed upon the triple expressions of Ras85D^V12.cUa.UAS+p53^VDRC.cUa+Pten^GD13500, Ras85D^V12.cUa.UAS+Apc^VDRC.cUa+Pten^GD13500 or Ras85D^V12.cUa.UAS+p53^VDRC.cUa+Apc^VDRC.cUa, the double expressions of Ras85D^V12.cUa.UAS+p53^VDRC.cUa, Ras85D^V12.cUa.UAS+Pten^GD13500 or Ras85D^V12.cUa.UAS+Apc^VDRC.cUa, all of which are more severe than upon the single expression of Ras85D^V12.cUa.UAS alone.

The co-expression for one week during adulthood of Pten^GD13500 and Apc^VDRC.cUa under the combined control of Scer\GAL4^byn-Gal4, Dicer-2 (for efficient RNAi) and Gal80[ts] (for the temporal control of expression) does not affect the replication of cells (i.e. BrU-positive) in the pylorus, as compared to controls.

The quadruple co-expression condition p53^VDRC.cUa+Pten^GD13500+Med^VDRC.cUa+Apc^VDRC.cUa also leads to a very mild cell dissemination through the body.

(Bangi et al., 2016)

Co-expression of hppy^Scer\UAS.cMa rescues the large wing phenotype of Pten^GD13500, Scer\GAL4^nub-AC-62 flies. Wing size and the number of cells is more similar to over-expression of hppy^Scer\UAS.cMa alone, while the cell size phenotype is intermediate.

(Resnik-Docampo and de Celis, 2011)

Xenogenetic Interactions

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Reference

Complementation and Rescue Data

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Mutant

Wild-type

Stocks (1)

VDRC

v35731

w¹¹¹⁸; P{GD13500}v35731

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Crossreferences

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

13500

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Pten^GD13500

(Singh et al., 2020)

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Secondary FlyBase IDs

References (15)

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